Novel Resolvin D1-Loaded Biologics as an Advanced Approach for Inflammation Control and Tissue Regeneration: Preparation and Characterization
<b>Background/Objectives:</b> Constant inflammation can be a detrimental response in bone regeneration. To regulate of the inflammatory response and synchronically promote rapid tissue regeneration is a vital clinical challenge. The urinary bladder matrix (UBM) and small intestinal submu...
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| author | Zhe Xing Jingwen Liang Yang Sun Jing Dai Jiazheng Cai Masahito Fujio Yiwen Xu Xiaoli An Ying Xue |
| author_facet | Zhe Xing Jingwen Liang Yang Sun Jing Dai Jiazheng Cai Masahito Fujio Yiwen Xu Xiaoli An Ying Xue |
| author_sort | Zhe Xing |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Constant inflammation can be a detrimental response in bone regeneration. To regulate of the inflammatory response and synchronically promote rapid tissue regeneration is a vital clinical challenge. The urinary bladder matrix (UBM) and small intestinal submucosa (SIS) composite are commonly used extracellular matrix (ECM) materials. We designed a novel drug-loaded membrane by integrating the biological matrix (BM) composed of UBM and SIS composites with Resolvin D1 (RvD1), an endogenous pro-resolving lipid mediator, using the lyophilization process. This membrane is referred to as BRL, an acronym for BM-RvD1-Lyophilization. <b>Methods</b>: In this study, the physicochemical properties of the membranes were characterized. Fluorescence staining and the CCK8 assay kit were utilized to assess biocompatibility. To evaluate the inflammatory resolution properties and osteogenic ability of osteoblasts, real-time quantitative PCR and ELISA were conducted. <b>Results</b>: BRL exhibited a more pronounced three-dimensional pore structure, demonstrating excellent physicochemical properties and enabling the slow release of RvD1. This approach improved the viability of MG63 osteoblast-like cells, reduced LPS-induced inflammation, and upregulated osteogenesis-related genes significantly. <b>Conclusions</b>: By integrating inflammation control capabilities into tissue regeneration materials, BRL effectively regulates the tissue regeneration microenvironment, thereby enhancing regeneration efficiency and positioning itself as an exceptional candidate for future tissue regeneration membranes. |
| format | Article |
| id | doaj-art-83b1be3977cc4e859c4bdf8bd4fb584a |
| institution | DOAJ |
| issn | 1999-4923 |
| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-83b1be3977cc4e859c4bdf8bd4fb584a2025-08-20T03:14:42ZengMDPI AGPharmaceutics1999-49232025-05-0117564310.3390/pharmaceutics17050643Novel Resolvin D1-Loaded Biologics as an Advanced Approach for Inflammation Control and Tissue Regeneration: Preparation and CharacterizationZhe Xing0Jingwen Liang1Yang Sun2Jing Dai3Jiazheng Cai4Masahito Fujio5Yiwen Xu6Xiaoli An7Ying Xue8School/Hospital of Stomatology, Lanzhou University, Lanzhou 730000, ChinaSchool/Hospital of Stomatology, Lanzhou University, Lanzhou 730000, ChinaZhuoruan Medical Technologies (Suzhou) Co., Ltd., Suzhou 215434, ChinaZhuoruan Medical Technologies (Suzhou) Co., Ltd., Suzhou 215434, ChinaDepartment of Medical Biology, Faculty of Health Sciences, UIT the Artic University of Norway, 9037 Tromso, NorwayDepartment of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya 464-0083, JapanZhuoruan Medical Technologies (Suzhou) Co., Ltd., Suzhou 215434, ChinaSchool/Hospital of Stomatology, Lanzhou University, Lanzhou 730000, ChinaDepartment of Clinical Dentistry, Faculty of Health Sciences, UIT the Artic University of Norway, 9037 Tromso, Norway<b>Background/Objectives:</b> Constant inflammation can be a detrimental response in bone regeneration. To regulate of the inflammatory response and synchronically promote rapid tissue regeneration is a vital clinical challenge. The urinary bladder matrix (UBM) and small intestinal submucosa (SIS) composite are commonly used extracellular matrix (ECM) materials. We designed a novel drug-loaded membrane by integrating the biological matrix (BM) composed of UBM and SIS composites with Resolvin D1 (RvD1), an endogenous pro-resolving lipid mediator, using the lyophilization process. This membrane is referred to as BRL, an acronym for BM-RvD1-Lyophilization. <b>Methods</b>: In this study, the physicochemical properties of the membranes were characterized. Fluorescence staining and the CCK8 assay kit were utilized to assess biocompatibility. To evaluate the inflammatory resolution properties and osteogenic ability of osteoblasts, real-time quantitative PCR and ELISA were conducted. <b>Results</b>: BRL exhibited a more pronounced three-dimensional pore structure, demonstrating excellent physicochemical properties and enabling the slow release of RvD1. This approach improved the viability of MG63 osteoblast-like cells, reduced LPS-induced inflammation, and upregulated osteogenesis-related genes significantly. <b>Conclusions</b>: By integrating inflammation control capabilities into tissue regeneration materials, BRL effectively regulates the tissue regeneration microenvironment, thereby enhancing regeneration efficiency and positioning itself as an exceptional candidate for future tissue regeneration membranes.https://www.mdpi.com/1999-4923/17/5/643urinary bladder matrixbiological matrixResolvin D1lyophilizationinflammation resolutionosteoblastic differentiation |
| spellingShingle | Zhe Xing Jingwen Liang Yang Sun Jing Dai Jiazheng Cai Masahito Fujio Yiwen Xu Xiaoli An Ying Xue Novel Resolvin D1-Loaded Biologics as an Advanced Approach for Inflammation Control and Tissue Regeneration: Preparation and Characterization Pharmaceutics urinary bladder matrix biological matrix Resolvin D1 lyophilization inflammation resolution osteoblastic differentiation |
| title | Novel Resolvin D1-Loaded Biologics as an Advanced Approach for Inflammation Control and Tissue Regeneration: Preparation and Characterization |
| title_full | Novel Resolvin D1-Loaded Biologics as an Advanced Approach for Inflammation Control and Tissue Regeneration: Preparation and Characterization |
| title_fullStr | Novel Resolvin D1-Loaded Biologics as an Advanced Approach for Inflammation Control and Tissue Regeneration: Preparation and Characterization |
| title_full_unstemmed | Novel Resolvin D1-Loaded Biologics as an Advanced Approach for Inflammation Control and Tissue Regeneration: Preparation and Characterization |
| title_short | Novel Resolvin D1-Loaded Biologics as an Advanced Approach for Inflammation Control and Tissue Regeneration: Preparation and Characterization |
| title_sort | novel resolvin d1 loaded biologics as an advanced approach for inflammation control and tissue regeneration preparation and characterization |
| topic | urinary bladder matrix biological matrix Resolvin D1 lyophilization inflammation resolution osteoblastic differentiation |
| url | https://www.mdpi.com/1999-4923/17/5/643 |
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