Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice
The emergence of novel highly pathogenic avian influenza viruses (HPAIVs) in migratory birds raises serious concerns as these viruses have the potential to spread during fall migration. We report the identification of novel HPAIV A(H5N8) clade 2.3.4.4 virus that was isolated from sick domestic duck...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Interdisciplinary Perspectives on Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/2019/4124865 |
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| author | Elena A. Prokopyeva Vsevolod A. Zinserling You-Chan Bae Yongkuk Kwon Olga G. Kurskaya Ivan A. Sobolev Peter M. Kozhin Andrey Komissarov Artem Fadeev Vladimir Petrov Alexander M. Shestopalov Kirill A. Sharshov |
| author_facet | Elena A. Prokopyeva Vsevolod A. Zinserling You-Chan Bae Yongkuk Kwon Olga G. Kurskaya Ivan A. Sobolev Peter M. Kozhin Andrey Komissarov Artem Fadeev Vladimir Petrov Alexander M. Shestopalov Kirill A. Sharshov |
| author_sort | Elena A. Prokopyeva |
| collection | DOAJ |
| description | The emergence of novel highly pathogenic avian influenza viruses (HPAIVs) in migratory birds raises serious concerns as these viruses have the potential to spread during fall migration. We report the identification of novel HPAIV A(H5N8) clade 2.3.4.4 virus that was isolated from sick domestic duck at commercial farm during the second wave of spread that began in October and affected poultry (ducks; chiсkens) in several European regions of Russia and Western Siberia in 2016. The strain was highly lethal in experimental infection of chickens and mice with IVPI = 2.34 and MLD50 = 1.3log10 EID50, accordingly. Inoculation of chickens with the HPAIV A/H5N8 demonstrated neuroinvasiveness, multiorgan failure, and death of chickens on the 3rd day post inoculation. Virus replicated in all collected organ samples in high viral titers with the highest titer in the brain (6.75±0.1 log10TCID50/ml). Effective virus replication was found in the following cells: neurons and glial cells of a brain; alveolar cells and macrophages of lungs; epithelial cells of a small intestine; hepatocytes and Kupffer cells of a liver; macrophages and endothelial cells of a spleen; and the tubular epithelial cells of kidneys. These findings advance our understanding of histopathological effect of A(H5N8) HPAIV infection. |
| format | Article |
| id | doaj-art-83a96631c25741f6b6896da4b9d15aaa |
| institution | OA Journals |
| issn | 1687-708X 1687-7098 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Interdisciplinary Perspectives on Infectious Diseases |
| spelling | doaj-art-83a96631c25741f6b6896da4b9d15aaa2025-08-20T02:09:07ZengWileyInterdisciplinary Perspectives on Infectious Diseases1687-708X1687-70982019-01-01201910.1155/2019/41248654124865Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and MiceElena A. Prokopyeva0Vsevolod A. Zinserling1You-Chan Bae2Yongkuk Kwon3Olga G. Kurskaya4Ivan A. Sobolev5Peter M. Kozhin6Andrey Komissarov7Artem Fadeev8Vladimir Petrov9Alexander M. Shestopalov10Kirill A. Sharshov11Department of Experimental Research, Federal Research Center for Basic and Translational Medicine, Novosibirsk 630117, RussiaInstitute of Experimental Medicine, Almazov National Federal Research Centre, Saint Petersburg 197341, RussiaAvian Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si 39660, Republic of KoreaAvian Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si 39660, Republic of KoreaDepartment of Experimental Research, Federal Research Center for Basic and Translational Medicine, Novosibirsk 630117, RussiaDepartment of Experimental Research, Federal Research Center for Basic and Translational Medicine, Novosibirsk 630117, RussiaDepartment of Experimental Research, Federal Research Center for Basic and Translational Medicine, Novosibirsk 630117, RussiaDepartment of etiology and epidemiology, Smorodintsev Research Institute of Influenza, Saint Petersburg 197376, RussiaDepartment of etiology and epidemiology, Smorodintsev Research Institute of Influenza, Saint Petersburg 197376, RussiaDepartment of Experimental Research, Federal Research Center for Basic and Translational Medicine, Novosibirsk 630117, RussiaDepartment of Experimental Research, Federal Research Center for Basic and Translational Medicine, Novosibirsk 630117, RussiaDepartment of Experimental Research, Federal Research Center for Basic and Translational Medicine, Novosibirsk 630117, RussiaThe emergence of novel highly pathogenic avian influenza viruses (HPAIVs) in migratory birds raises serious concerns as these viruses have the potential to spread during fall migration. We report the identification of novel HPAIV A(H5N8) clade 2.3.4.4 virus that was isolated from sick domestic duck at commercial farm during the second wave of spread that began in October and affected poultry (ducks; chiсkens) in several European regions of Russia and Western Siberia in 2016. The strain was highly lethal in experimental infection of chickens and mice with IVPI = 2.34 and MLD50 = 1.3log10 EID50, accordingly. Inoculation of chickens with the HPAIV A/H5N8 demonstrated neuroinvasiveness, multiorgan failure, and death of chickens on the 3rd day post inoculation. Virus replicated in all collected organ samples in high viral titers with the highest titer in the brain (6.75±0.1 log10TCID50/ml). Effective virus replication was found in the following cells: neurons and glial cells of a brain; alveolar cells and macrophages of lungs; epithelial cells of a small intestine; hepatocytes and Kupffer cells of a liver; macrophages and endothelial cells of a spleen; and the tubular epithelial cells of kidneys. These findings advance our understanding of histopathological effect of A(H5N8) HPAIV infection.http://dx.doi.org/10.1155/2019/4124865 |
| spellingShingle | Elena A. Prokopyeva Vsevolod A. Zinserling You-Chan Bae Yongkuk Kwon Olga G. Kurskaya Ivan A. Sobolev Peter M. Kozhin Andrey Komissarov Artem Fadeev Vladimir Petrov Alexander M. Shestopalov Kirill A. Sharshov Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice Interdisciplinary Perspectives on Infectious Diseases |
| title | Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice |
| title_full | Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice |
| title_fullStr | Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice |
| title_full_unstemmed | Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice |
| title_short | Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice |
| title_sort | pathology of a h5n8 clade 2 3 4 4 virus in experimentally infected chickens and mice |
| url | http://dx.doi.org/10.1155/2019/4124865 |
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