Stability of Folate and Vitamin B12 in Human Serum after Long-Term Storage: A Follow-Up after 13 Years
In epidemiological and nutrition research, it is very important to evaluate the stability of biomarkers as function of both storage time and temperature. In this study, the stability of folate and vitamin B12 in human serum samples has been tested after long-term storage at −80°C up to 13 years. Ser...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2018/9834181 |
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| author | Eugène H. J. M. Jansen Piet K. Beekhof |
| author_facet | Eugène H. J. M. Jansen Piet K. Beekhof |
| author_sort | Eugène H. J. M. Jansen |
| collection | DOAJ |
| description | In epidemiological and nutrition research, it is very important to evaluate the stability of biomarkers as function of both storage time and temperature. In this study, the stability of folate and vitamin B12 in human serum samples has been tested after long-term storage at −80°C up to 13 years. Serum samples of 16 individuals were used in this study. The concentration of folate and vitamin B12 has been determined at t=0 and at 1, 8, and 13 years after storage at −80°C. The folate concentrations in serum samples remained stable at −80°C. The concentration of vitamin B12 was decreasing during the time of the study to about 50%. The correlation of the folate and also of the vitamin B12 concentrations in the stored samples compared with the starting values was still good. Therefore, although the concentration of vitamin B12 decreased upon storage, reliable comparative analyses can still be performed. |
| format | Article |
| id | doaj-art-83a60a681a7649378948c365d7add81f |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-83a60a681a7649378948c365d7add81f2025-02-03T06:01:40ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322018-01-01201810.1155/2018/98341819834181Stability of Folate and Vitamin B12 in Human Serum after Long-Term Storage: A Follow-Up after 13 YearsEugène H. J. M. Jansen0Piet K. Beekhof1Centre for Health Protection, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, NetherlandsCentre for Health Protection, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, NetherlandsIn epidemiological and nutrition research, it is very important to evaluate the stability of biomarkers as function of both storage time and temperature. In this study, the stability of folate and vitamin B12 in human serum samples has been tested after long-term storage at −80°C up to 13 years. Serum samples of 16 individuals were used in this study. The concentration of folate and vitamin B12 has been determined at t=0 and at 1, 8, and 13 years after storage at −80°C. The folate concentrations in serum samples remained stable at −80°C. The concentration of vitamin B12 was decreasing during the time of the study to about 50%. The correlation of the folate and also of the vitamin B12 concentrations in the stored samples compared with the starting values was still good. Therefore, although the concentration of vitamin B12 decreased upon storage, reliable comparative analyses can still be performed.http://dx.doi.org/10.1155/2018/9834181 |
| spellingShingle | Eugène H. J. M. Jansen Piet K. Beekhof Stability of Folate and Vitamin B12 in Human Serum after Long-Term Storage: A Follow-Up after 13 Years Journal of Nutrition and Metabolism |
| title | Stability of Folate and Vitamin B12 in Human Serum after Long-Term Storage: A Follow-Up after 13 Years |
| title_full | Stability of Folate and Vitamin B12 in Human Serum after Long-Term Storage: A Follow-Up after 13 Years |
| title_fullStr | Stability of Folate and Vitamin B12 in Human Serum after Long-Term Storage: A Follow-Up after 13 Years |
| title_full_unstemmed | Stability of Folate and Vitamin B12 in Human Serum after Long-Term Storage: A Follow-Up after 13 Years |
| title_short | Stability of Folate and Vitamin B12 in Human Serum after Long-Term Storage: A Follow-Up after 13 Years |
| title_sort | stability of folate and vitamin b12 in human serum after long term storage a follow up after 13 years |
| url | http://dx.doi.org/10.1155/2018/9834181 |
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