Overexpression of soybean GmDHN9 gene enhances drought resistance of transgenic Arabidopsis
Soybean is one of the important oil crops and a major source of protein and lipids. Drought can cause severe soybean yields. Dehydrin protein (DHN) is a subfamily of LEA proteins that play an important role in plant responses to abiotic stresses. In this study, the soybean GmDHN9 gene was cloned and...
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| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | GM Crops & Food |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21645698.2024.2327116 |
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| _version_ | 1850116503233363968 |
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| author | Jiayi Fan Yuzhe Zhang Hongji Sun Ruijie Duan Yushi Jiang Xinyu Wang Yao Sun Zhipeng Luo Peiwu Wang Shuyan Guan Siyan Liu Xuhong Fan Peng Jiao Yunpeng Wang Jinhui Yang Zunyue Zhang Huiwei Yu |
| author_facet | Jiayi Fan Yuzhe Zhang Hongji Sun Ruijie Duan Yushi Jiang Xinyu Wang Yao Sun Zhipeng Luo Peiwu Wang Shuyan Guan Siyan Liu Xuhong Fan Peng Jiao Yunpeng Wang Jinhui Yang Zunyue Zhang Huiwei Yu |
| author_sort | Jiayi Fan |
| collection | DOAJ |
| description | Soybean is one of the important oil crops and a major source of protein and lipids. Drought can cause severe soybean yields. Dehydrin protein (DHN) is a subfamily of LEA proteins that play an important role in plant responses to abiotic stresses. In this study, the soybean GmDHN9 gene was cloned and induced under a variety of abiotic stresses. Results showed that the GmDHN9 gene response was more pronounced under drought induction. Subcellular localization results indicated that the protein was localized in the cytoplasm. The role of transgenic Arabidopsis plants in drought stress response was further studied. Under drought stress, the germination rate, root length, chlorophyll, proline, relative water content, and antioxidant enzyme content of transgenic Arabidopsis thaliana transgenic genes were higher than those of wild-type plants, and transgenic plants contained less O2−, H2O2 and MDA contents. In short, the GmDHN9 gene can regulate the homeostasis of ROS and enhance the drought resistance of plants. |
| format | Article |
| id | doaj-art-839d5aa06fc042189b667cfb1295eef1 |
| institution | OA Journals |
| issn | 2164-5698 2164-5701 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | GM Crops & Food |
| spelling | doaj-art-839d5aa06fc042189b667cfb1295eef12025-08-20T02:36:19ZengTaylor & Francis GroupGM Crops & Food2164-56982164-57012024-12-0115111812910.1080/21645698.2024.2327116Overexpression of soybean GmDHN9 gene enhances drought resistance of transgenic ArabidopsisJiayi Fan0Yuzhe Zhang1Hongji Sun2Ruijie Duan3Yushi Jiang4Xinyu Wang5Yao Sun6Zhipeng Luo7Peiwu Wang8Shuyan Guan9Siyan Liu10Xuhong Fan11Peng Jiao12Yunpeng Wang13Jinhui Yang14Zunyue Zhang15Huiwei Yu16College of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaSoybean Research Institute, Jilin Academy of Agricultural Sciences, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaDaan Branch of Baicheng City Tobacco company of Jilin Province, Baicheng, ChinaChangchun Vocational and Technical College, Changchun Vocational Institute of Technology, changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaSoybean is one of the important oil crops and a major source of protein and lipids. Drought can cause severe soybean yields. Dehydrin protein (DHN) is a subfamily of LEA proteins that play an important role in plant responses to abiotic stresses. In this study, the soybean GmDHN9 gene was cloned and induced under a variety of abiotic stresses. Results showed that the GmDHN9 gene response was more pronounced under drought induction. Subcellular localization results indicated that the protein was localized in the cytoplasm. The role of transgenic Arabidopsis plants in drought stress response was further studied. Under drought stress, the germination rate, root length, chlorophyll, proline, relative water content, and antioxidant enzyme content of transgenic Arabidopsis thaliana transgenic genes were higher than those of wild-type plants, and transgenic plants contained less O2−, H2O2 and MDA contents. In short, the GmDHN9 gene can regulate the homeostasis of ROS and enhance the drought resistance of plants.https://www.tandfonline.com/doi/10.1080/21645698.2024.2327116dehydrindrought resistancefunctiongene expressionreactive oxygen species |
| spellingShingle | Jiayi Fan Yuzhe Zhang Hongji Sun Ruijie Duan Yushi Jiang Xinyu Wang Yao Sun Zhipeng Luo Peiwu Wang Shuyan Guan Siyan Liu Xuhong Fan Peng Jiao Yunpeng Wang Jinhui Yang Zunyue Zhang Huiwei Yu Overexpression of soybean GmDHN9 gene enhances drought resistance of transgenic Arabidopsis GM Crops & Food dehydrin drought resistance function gene expression reactive oxygen species |
| title | Overexpression of soybean GmDHN9 gene enhances drought resistance of transgenic Arabidopsis |
| title_full | Overexpression of soybean GmDHN9 gene enhances drought resistance of transgenic Arabidopsis |
| title_fullStr | Overexpression of soybean GmDHN9 gene enhances drought resistance of transgenic Arabidopsis |
| title_full_unstemmed | Overexpression of soybean GmDHN9 gene enhances drought resistance of transgenic Arabidopsis |
| title_short | Overexpression of soybean GmDHN9 gene enhances drought resistance of transgenic Arabidopsis |
| title_sort | overexpression of soybean gmdhn9 gene enhances drought resistance of transgenic arabidopsis |
| topic | dehydrin drought resistance function gene expression reactive oxygen species |
| url | https://www.tandfonline.com/doi/10.1080/21645698.2024.2327116 |
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