Analysis of Klebsiella Pneumonia and Resistance Genes Coding and Carbapenemase Production in National Referral Hospital Wastewater
Hospitals, as a source of transmission and spread of bacteria carrying antibiotic-resistant genes (ARGs) as well as a high source of antibiotic compounds, are a significant reservoir of antibiotic-resistant gene transfer that causes bacteria to develop multidrug resistance (MDR). Klebsiella pneumoni...
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| Format: | Article |
| Language: | Indonesian |
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Politeknik Kesehatan Tanjung Karang
2023-11-01
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| Series: | Jurnal Kesehatan |
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| Online Access: | https://ejurnal.poltekkes-tjk.ac.id/index.php/JK/article/view/4147 |
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| author | Glory Gelarich Simanjuntak Anis Karuniawati Yulia Rosa Saharman Windi Muziasari |
| author_facet | Glory Gelarich Simanjuntak Anis Karuniawati Yulia Rosa Saharman Windi Muziasari |
| author_sort | Glory Gelarich Simanjuntak |
| collection | DOAJ |
| description | Hospitals, as a source of transmission and spread of bacteria carrying antibiotic-resistant genes (ARGs) as well as a high source of antibiotic compounds, are a significant reservoir of antibiotic-resistant gene transfer that causes bacteria to develop multidrug resistance (MDR). Klebsiella pneumonia is a bacterium often found in wastewater with a high level of Extended Spectrum β-lactamase (ESBL) and carbapenemase resistance. This study aimed to determine Klebsiella pneumonia and resistance genes encoding ESBL and carbapenemase in hospital wastewater to obtain primary Antimicrobial Resistance (AMR) data in the environment, which was first conducted in Indonesia. The resistance gene detection method was developed using High Throughput Real-Time and conventional Real-Time (HT-RT PCR). The data obtained were relative abundance and copy number. The results showed that blaTEM and blaNDM were the highest ARGs. Conventional Real-Time PCR results showed better sensitivity in detecting K. pneumoniae and ARGs than HT-RT PCR. The detection of K. pneumoniae and ARGs in RSCM wastewater in Indonesia indicates the need to improve the handling of RSCM WWTP to monitor the number of microbial resistances to antibiotics. |
| format | Article |
| id | doaj-art-83906acc6be94f09921b344dc2b92eac |
| institution | Kabale University |
| issn | 2086-7751 2548-5695 |
| language | Indonesian |
| publishDate | 2023-11-01 |
| publisher | Politeknik Kesehatan Tanjung Karang |
| record_format | Article |
| series | Jurnal Kesehatan |
| spelling | doaj-art-83906acc6be94f09921b344dc2b92eac2025-01-22T14:13:52ZindPoliteknik Kesehatan Tanjung KarangJurnal Kesehatan2086-77512548-56952023-11-0114352853710.26630/jk.v14i3.41473475Analysis of Klebsiella Pneumonia and Resistance Genes Coding and Carbapenemase Production in National Referral Hospital WastewaterGlory Gelarich Simanjuntak0Anis Karuniawati1Yulia Rosa Saharman2Windi Muziasari3Master’s Programme in Biomedical Sciences, Faculty of Medicine, Universitas IndonesiaDepartment of Microbilogy, Faculty of Medicine, Universitas IndonesiaDepartment of Microbilogy, Faculty of Medicine, Universitas IndonesiaResistomap OyHospitals, as a source of transmission and spread of bacteria carrying antibiotic-resistant genes (ARGs) as well as a high source of antibiotic compounds, are a significant reservoir of antibiotic-resistant gene transfer that causes bacteria to develop multidrug resistance (MDR). Klebsiella pneumonia is a bacterium often found in wastewater with a high level of Extended Spectrum β-lactamase (ESBL) and carbapenemase resistance. This study aimed to determine Klebsiella pneumonia and resistance genes encoding ESBL and carbapenemase in hospital wastewater to obtain primary Antimicrobial Resistance (AMR) data in the environment, which was first conducted in Indonesia. The resistance gene detection method was developed using High Throughput Real-Time and conventional Real-Time (HT-RT PCR). The data obtained were relative abundance and copy number. The results showed that blaTEM and blaNDM were the highest ARGs. Conventional Real-Time PCR results showed better sensitivity in detecting K. pneumoniae and ARGs than HT-RT PCR. The detection of K. pneumoniae and ARGs in RSCM wastewater in Indonesia indicates the need to improve the handling of RSCM WWTP to monitor the number of microbial resistances to antibiotics.https://ejurnal.poltekkes-tjk.ac.id/index.php/JK/article/view/4147antibiotic-resistantenvironmentesbl. |
| spellingShingle | Glory Gelarich Simanjuntak Anis Karuniawati Yulia Rosa Saharman Windi Muziasari Analysis of Klebsiella Pneumonia and Resistance Genes Coding and Carbapenemase Production in National Referral Hospital Wastewater Jurnal Kesehatan antibiotic-resistant environment esbl. |
| title | Analysis of Klebsiella Pneumonia and Resistance Genes Coding and Carbapenemase Production in National Referral Hospital Wastewater |
| title_full | Analysis of Klebsiella Pneumonia and Resistance Genes Coding and Carbapenemase Production in National Referral Hospital Wastewater |
| title_fullStr | Analysis of Klebsiella Pneumonia and Resistance Genes Coding and Carbapenemase Production in National Referral Hospital Wastewater |
| title_full_unstemmed | Analysis of Klebsiella Pneumonia and Resistance Genes Coding and Carbapenemase Production in National Referral Hospital Wastewater |
| title_short | Analysis of Klebsiella Pneumonia and Resistance Genes Coding and Carbapenemase Production in National Referral Hospital Wastewater |
| title_sort | analysis of klebsiella pneumonia and resistance genes coding and carbapenemase production in national referral hospital wastewater |
| topic | antibiotic-resistant environment esbl. |
| url | https://ejurnal.poltekkes-tjk.ac.id/index.php/JK/article/view/4147 |
| work_keys_str_mv | AT glorygelarichsimanjuntak analysisofklebsiellapneumoniaandresistancegenescodingandcarbapenemaseproductioninnationalreferralhospitalwastewater AT aniskaruniawati analysisofklebsiellapneumoniaandresistancegenescodingandcarbapenemaseproductioninnationalreferralhospitalwastewater AT yuliarosasaharman analysisofklebsiellapneumoniaandresistancegenescodingandcarbapenemaseproductioninnationalreferralhospitalwastewater AT windimuziasari analysisofklebsiellapneumoniaandresistancegenescodingandcarbapenemaseproductioninnationalreferralhospitalwastewater |