Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells
Abstract Lysophosphatidylethanolamine (LPE) is a bioactive lipid mediator involved in diverse cellular functions. In this study, we investigated the effects of three LPE species, 1-palmitoyl LPE (16:0 LPE), 1-stearoyl LPE (18:0 LPE), and 1-oleoyl LPE (18:1 LPE) on pre-osteoblast MC3T3-E1 cells. All...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-84176-8 |
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| author | Fumiaki Makiyama Shiori Kawase Aoi William Omi Yusuke Tanikawa Taishi Kotani Teruki Shirayama Naoyuki Nishimura Taiga Kurihara Naoto Saito Jun Takahashi Takeshi Uemura |
| author_facet | Fumiaki Makiyama Shiori Kawase Aoi William Omi Yusuke Tanikawa Taishi Kotani Teruki Shirayama Naoyuki Nishimura Taiga Kurihara Naoto Saito Jun Takahashi Takeshi Uemura |
| author_sort | Fumiaki Makiyama |
| collection | DOAJ |
| description | Abstract Lysophosphatidylethanolamine (LPE) is a bioactive lipid mediator involved in diverse cellular functions. In this study, we investigated the effects of three LPE species, 1-palmitoyl LPE (16:0 LPE), 1-stearoyl LPE (18:0 LPE), and 1-oleoyl LPE (18:1 LPE) on pre-osteoblast MC3T3-E1 cells. All LPE species stimulated cell proliferation and activated the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) 1/2. MAPK/ERK1/2 activation by 16:0 LPE and 18:1 LPE was inhibited by the Gq/11 inhibitor YM-254890, while activation by 18:0 LPE was blocked by the Gi/o inhibitor pertussis toxin. Intracellular Ca2+ transients were triggered by 16:0 LPE and 18:1 LPE but not by 18:0 LPE, with YM-254890 suppressing these responses. These results suggest that 16:0 and 18:1 LPE act via Gq/11-coupled G protein coupled receptors (GPCRs), and 18:0 LPE acts via Gi/o-coupled GPCRs. Furthermore, receptor desensitization experiments suggested that each LPE acts through distinct GPCRs. Interestingly, 18:0 LPE suppressed osteogenic differentiation, reducing mineralization, alkaline phosphatase activity, and osteogenic gene expression, whereas 16:0 LPE and 18:1 LPE had no such effects. These results suggest the physiological significance of LPEs in bone formation and indicate that different LPE species and their receptors play distinctive roles in this process. |
| format | Article |
| id | doaj-art-838c8a9fd9934dbb98a70547275e3bed |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-838c8a9fd9934dbb98a70547275e3bed2025-01-05T12:23:27ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-024-84176-8Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cellsFumiaki Makiyama0Shiori Kawase1Aoi William Omi2Yusuke Tanikawa3Taishi Kotani4Teruki Shirayama5Naoyuki Nishimura6Taiga Kurihara7Naoto Saito8Jun Takahashi9Takeshi Uemura10Department of Orthopedic Surgery, Shinshu University School of MedicineDivision of Gene Research, Research Center for Advanced Science and Technology, Shinshu UniversityDepartment of Biomedical Engineering, Graduate School of Medicine, Science and Technology, Shinshu UniversityDepartment of Orthopedic Surgery, Shinshu University School of MedicineDepartment of Biomedical Engineering, Graduate School of Science and Technology, Shinshu UniversityDepartment of Orthopedic Surgery, Shinshu University School of MedicineInstitute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu UniversityDivision of Microbiology and Molecular Cell Biology, Nihon Pharmaceutical UniversityInstitute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu UniversityDepartment of Orthopedic Surgery, Shinshu University School of MedicineDepartment of Biomedical Engineering, Graduate School of Medicine, Science and Technology, Shinshu UniversityAbstract Lysophosphatidylethanolamine (LPE) is a bioactive lipid mediator involved in diverse cellular functions. In this study, we investigated the effects of three LPE species, 1-palmitoyl LPE (16:0 LPE), 1-stearoyl LPE (18:0 LPE), and 1-oleoyl LPE (18:1 LPE) on pre-osteoblast MC3T3-E1 cells. All LPE species stimulated cell proliferation and activated the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) 1/2. MAPK/ERK1/2 activation by 16:0 LPE and 18:1 LPE was inhibited by the Gq/11 inhibitor YM-254890, while activation by 18:0 LPE was blocked by the Gi/o inhibitor pertussis toxin. Intracellular Ca2+ transients were triggered by 16:0 LPE and 18:1 LPE but not by 18:0 LPE, with YM-254890 suppressing these responses. These results suggest that 16:0 and 18:1 LPE act via Gq/11-coupled G protein coupled receptors (GPCRs), and 18:0 LPE acts via Gi/o-coupled GPCRs. Furthermore, receptor desensitization experiments suggested that each LPE acts through distinct GPCRs. Interestingly, 18:0 LPE suppressed osteogenic differentiation, reducing mineralization, alkaline phosphatase activity, and osteogenic gene expression, whereas 16:0 LPE and 18:1 LPE had no such effects. These results suggest the physiological significance of LPEs in bone formation and indicate that different LPE species and their receptors play distinctive roles in this process.https://doi.org/10.1038/s41598-024-84176-8LysophosphatidylethanolaminePre-osteoblast cellProliferationDifferentiationG protein-coupled receptors |
| spellingShingle | Fumiaki Makiyama Shiori Kawase Aoi William Omi Yusuke Tanikawa Taishi Kotani Teruki Shirayama Naoyuki Nishimura Taiga Kurihara Naoto Saito Jun Takahashi Takeshi Uemura Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells Scientific Reports Lysophosphatidylethanolamine Pre-osteoblast cell Proliferation Differentiation G protein-coupled receptors |
| title | Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells |
| title_full | Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells |
| title_fullStr | Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells |
| title_full_unstemmed | Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells |
| title_short | Differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre-osteoblast MC3T3-E1 cells |
| title_sort | differential effects of structurally different lysophosphatidylethanolamine species on proliferation and differentiation in pre osteoblast mc3t3 e1 cells |
| topic | Lysophosphatidylethanolamine Pre-osteoblast cell Proliferation Differentiation G protein-coupled receptors |
| url | https://doi.org/10.1038/s41598-024-84176-8 |
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