Investigation, characterization and prevalence assessment of various fungalcontaminants in sugarcane in vitro cultures

Microbial contamination poses a significant challenge during the tissue culture process. The current investigation focuses on examining fungal contaminants in in vitro cultures of two distinct sugarcane varieties, CoN 13073 and CoN 707, aiming at their detection,  isolation,  and  identification.  Th...

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Main Authors: Hariprasath M, Bhukya Srinivas, Shivangi Kansara, Priya John
Format: Article
Language:English
Published: Society for Sugarcane Research and Development 2025-05-01
Series:Journal of Sugarcane Research
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Online Access:https://epubs.icar.org.in/index.php/JSR/article/view/154148
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author Hariprasath M
Bhukya Srinivas
Shivangi Kansara
Priya John
author_facet Hariprasath M
Bhukya Srinivas
Shivangi Kansara
Priya John
author_sort Hariprasath M
collection DOAJ
description Microbial contamination poses a significant challenge during the tissue culture process. The current investigation focuses on examining fungal contaminants in in vitro cultures of two distinct sugarcane varieties, CoN 13073 and CoN 707, aiming at their detection,  isolation,  and  identification.  The  study  employed  the  fungal  tip  isolation  method  to  isolate  and   identify  fungal contaminants. Six different fungal species were identified, including Aspergillus flavus Link, Penicillium sp., Macrophomina phaseolina (Tassi) Goid., Curvularia sp., Rhizopus sp., and an aseptate sterile fungus, based on their cultural and morphological characteristics from contaminated cultures of the two sugarcane varieties. The frequency of fungal contaminants associated with in vitro cultures of sugarcane was assessed in both varieties at 15-day intervals from explant inoculation to 60 days. Among the six isolates, A. flavus (21.62-33.33%) was the most prevalent across all time intervals in both varieties, followed by Penicillium sp. (14.81-31.58%), Curvularia sp. (0-26.32%), M. phaseolina (0-21.62%), and Rhizopus sp. (15-19.05%). The least frequent fungal contaminant observed was the aseptate sterile fungus (4.76-11.76%) at all time intervals in both varieties.
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spelling doaj-art-838ae3b8152d47a78044fcdab16be5e02025-08-20T02:02:26ZengSociety for Sugarcane Research and DevelopmentJournal of Sugarcane Research2249-927X2582-47672025-05-0113110.37580/JSR.2023.1.13.67-74Investigation, characterization and prevalence assessment of various fungalcontaminants in sugarcane in vitro culturesHariprasath M0Bhukya Srinivas1Shivangi Kansara2Priya John3Department of Plant Pathology, C. P. College of Agriculture, S. D. Agricultural University, S. K. Nagar, Gujarat.Navsari Agricultural UniversityNavsari Agricultural UniversityNavsari Agricultural University Microbial contamination poses a significant challenge during the tissue culture process. The current investigation focuses on examining fungal contaminants in in vitro cultures of two distinct sugarcane varieties, CoN 13073 and CoN 707, aiming at their detection,  isolation,  and  identification.  The  study  employed  the  fungal  tip  isolation  method  to  isolate  and   identify  fungal contaminants. Six different fungal species were identified, including Aspergillus flavus Link, Penicillium sp., Macrophomina phaseolina (Tassi) Goid., Curvularia sp., Rhizopus sp., and an aseptate sterile fungus, based on their cultural and morphological characteristics from contaminated cultures of the two sugarcane varieties. The frequency of fungal contaminants associated with in vitro cultures of sugarcane was assessed in both varieties at 15-day intervals from explant inoculation to 60 days. Among the six isolates, A. flavus (21.62-33.33%) was the most prevalent across all time intervals in both varieties, followed by Penicillium sp. (14.81-31.58%), Curvularia sp. (0-26.32%), M. phaseolina (0-21.62%), and Rhizopus sp. (15-19.05%). The least frequent fungal contaminant observed was the aseptate sterile fungus (4.76-11.76%) at all time intervals in both varieties. https://epubs.icar.org.in/index.php/JSR/article/view/154148Sugarcane; Contamination; Tissue culture; Micropropagation; Fungal frequency
spellingShingle Hariprasath M
Bhukya Srinivas
Shivangi Kansara
Priya John
Investigation, characterization and prevalence assessment of various fungalcontaminants in sugarcane in vitro cultures
Journal of Sugarcane Research
Sugarcane; Contamination; Tissue culture; Micropropagation; Fungal frequency
title Investigation, characterization and prevalence assessment of various fungalcontaminants in sugarcane in vitro cultures
title_full Investigation, characterization and prevalence assessment of various fungalcontaminants in sugarcane in vitro cultures
title_fullStr Investigation, characterization and prevalence assessment of various fungalcontaminants in sugarcane in vitro cultures
title_full_unstemmed Investigation, characterization and prevalence assessment of various fungalcontaminants in sugarcane in vitro cultures
title_short Investigation, characterization and prevalence assessment of various fungalcontaminants in sugarcane in vitro cultures
title_sort investigation characterization and prevalence assessment of various fungalcontaminants in sugarcane in vitro cultures
topic Sugarcane; Contamination; Tissue culture; Micropropagation; Fungal frequency
url https://epubs.icar.org.in/index.php/JSR/article/view/154148
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AT shivangikansara investigationcharacterizationandprevalenceassessmentofvariousfungalcontaminantsinsugarcaneinvitrocultures
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