A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays
Abstract Various pathogens from oomycete, fungi, and bacteria kingdoms can infect potato and significantly reduce potato yield. The early diagnosis of potato pathogens is important for tracing disease epidemics and the subsequent disease management. Loop-mediated isothermal amplification (LAMP) is a...
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BMC
2024-12-01
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| Series: | Phytopathology Research |
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| Online Access: | https://doi.org/10.1186/s42483-024-00284-3 |
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| author | Xinjie Zhang Han Chen Jinbin Wu Xiao Wang Shuaishuai Wang Jingqi Chen Qianpeng Yu Zhenxin Zhang Xiaobo Zheng Juan Zeng Suomeng Dong |
| author_facet | Xinjie Zhang Han Chen Jinbin Wu Xiao Wang Shuaishuai Wang Jingqi Chen Qianpeng Yu Zhenxin Zhang Xiaobo Zheng Juan Zeng Suomeng Dong |
| author_sort | Xinjie Zhang |
| collection | DOAJ |
| description | Abstract Various pathogens from oomycete, fungi, and bacteria kingdoms can infect potato and significantly reduce potato yield. The early diagnosis of potato pathogens is important for tracing disease epidemics and the subsequent disease management. Loop-mediated isothermal amplification (LAMP) is a critical technique for pathogen detection, but available LAMP assays do not effectively meet the requirement of field diagnoses due to complexities including co-infection of different pathogens. Hence, this study aims to develop integrated-LAMP assays (iLAMPs) for simultaneous detection of eight common potato pathogens and apply iLAMPs to pathogen detection in field samples from the four main potato-growing regions of China in 2023. Therefore, eight sets of primers showing gene- and genus-specificity were designed and used for iLAMPs to determine their specificity, sensitivity, and visualization. Subsequently, iLAMPs-mediated pathogen detection revealed that 72.82% of 206 diseased leaves and 84.94% of 239 diseased tubers carry more than one pathogen. The detection rate for each pathogen significantly varies from 1.94 to 65.53% in diseased leaves, and ranges from 26.78 to 52.72% in diseased tubers, respectively. In addition, the detection rate of Phytophthora infestans and Alternaria solani positively correlates in both leaves and tubers, especially for those samples from the southwestern and southern regions. Taken together, iLAMPs developed in this study enables simultaneous detection of eight common potato pathogens from field samples and may have broad applications in early management of potato diseases. |
| format | Article |
| id | doaj-art-83828d8fe062479892eeea7e8a27b018 |
| institution | OA Journals |
| issn | 2524-4167 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
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| series | Phytopathology Research |
| spelling | doaj-art-83828d8fe062479892eeea7e8a27b0182025-08-20T02:37:57ZengBMCPhytopathology Research2524-41672024-12-016111410.1186/s42483-024-00284-3A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assaysXinjie Zhang0Han Chen1Jinbin Wu2Xiao Wang3Shuaishuai Wang4Jingqi Chen5Qianpeng Yu6Zhenxin Zhang7Xiaobo Zheng8Juan Zeng9Suomeng Dong10Department of Plant Pathology, Nanjing Agricultural UniversityDepartment of Plant Pathology, Nanjing Agricultural UniversityDepartment of Plant Pathology, Nanjing Agricultural UniversityDepartment of Plant Pathology, Nanjing Agricultural UniversityDepartment of Plant Pathology, Nanjing Agricultural UniversityDepartment of Plant Pathology, Nanjing Agricultural UniversityDepartment of Plant Pathology, Nanjing Agricultural UniversityInternational Potato Center (CIP), CIP China Center Asia PacificDepartment of Plant Pathology, Nanjing Agricultural UniversityNational Agro-Tech Extension and Service CenterDepartment of Plant Pathology, Nanjing Agricultural UniversityAbstract Various pathogens from oomycete, fungi, and bacteria kingdoms can infect potato and significantly reduce potato yield. The early diagnosis of potato pathogens is important for tracing disease epidemics and the subsequent disease management. Loop-mediated isothermal amplification (LAMP) is a critical technique for pathogen detection, but available LAMP assays do not effectively meet the requirement of field diagnoses due to complexities including co-infection of different pathogens. Hence, this study aims to develop integrated-LAMP assays (iLAMPs) for simultaneous detection of eight common potato pathogens and apply iLAMPs to pathogen detection in field samples from the four main potato-growing regions of China in 2023. Therefore, eight sets of primers showing gene- and genus-specificity were designed and used for iLAMPs to determine their specificity, sensitivity, and visualization. Subsequently, iLAMPs-mediated pathogen detection revealed that 72.82% of 206 diseased leaves and 84.94% of 239 diseased tubers carry more than one pathogen. The detection rate for each pathogen significantly varies from 1.94 to 65.53% in diseased leaves, and ranges from 26.78 to 52.72% in diseased tubers, respectively. In addition, the detection rate of Phytophthora infestans and Alternaria solani positively correlates in both leaves and tubers, especially for those samples from the southwestern and southern regions. Taken together, iLAMPs developed in this study enables simultaneous detection of eight common potato pathogens from field samples and may have broad applications in early management of potato diseases.https://doi.org/10.1186/s42483-024-00284-3PotatoPathogen detectionLoop-mediated isothermal amplificationPhytophthora infestansAlternaria solani |
| spellingShingle | Xinjie Zhang Han Chen Jinbin Wu Xiao Wang Shuaishuai Wang Jingqi Chen Qianpeng Yu Zhenxin Zhang Xiaobo Zheng Juan Zeng Suomeng Dong A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays Phytopathology Research Potato Pathogen detection Loop-mediated isothermal amplification Phytophthora infestans Alternaria solani |
| title | A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays |
| title_full | A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays |
| title_fullStr | A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays |
| title_full_unstemmed | A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays |
| title_short | A field survey of eight common potato pathogens in China based on integrated-loop-mediated isothermal amplification assays |
| title_sort | field survey of eight common potato pathogens in china based on integrated loop mediated isothermal amplification assays |
| topic | Potato Pathogen detection Loop-mediated isothermal amplification Phytophthora infestans Alternaria solani |
| url | https://doi.org/10.1186/s42483-024-00284-3 |
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