Multiple strategies were adopted to optimize the enzymatic characteristics and improve the expression of bovine chymosin BtChy in Kluyveromyces lactis for cheese production

Multiple strategies were adopted to optimize the enzymatic characteristics and improve the expression of bovine chymosin BtChy in Kluyveromyces lactis for cheese production

Chymosin (EC3.4.23.4), primarily sourced from calf abomasum, serves as a conventional coagulant in milk curdling during cheese production. To improve the enzymatic properties and enhance the expression of calf chymosin (BtChy) in Kluyveromyces lactis to meet the demands of the cheese industry, the i...

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Bibliographic Details
Main Authors: Ying Han, Liu-Qun Zhang, De-Ming Rao, Lei Lei, Jiang-Ke Yang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Microbiology
Subjects:
calf chymosin
in silico engineering
signal peptide
promoter
gene dosage
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1605229/full
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