Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi
Artemisia argyi H. Lév. & Vaniot is an important traditional Chinese medicinal plant known for its volatile oils, which are the main active components of A. argyi, including monoterpenes, sesquiterpenes and their derivatives. Despite its medicinal significance, the biosynthesis of sesquiterp...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2025-03-01
|
| Series: | Synthetic and Systems Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X24001248 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849715535698198528 |
|---|---|
| author | Zhengping Li Yuhong Gan Changyu Gou Qiongyu Ye Yang Wu Yuhong Wu Tingxing Yang Baolian Fan Aijia Ji Qi Shen Lixin Duan |
| author_facet | Zhengping Li Yuhong Gan Changyu Gou Qiongyu Ye Yang Wu Yuhong Wu Tingxing Yang Baolian Fan Aijia Ji Qi Shen Lixin Duan |
| author_sort | Zhengping Li |
| collection | DOAJ |
| description | Artemisia argyi H. Lév. & Vaniot is an important traditional Chinese medicinal plant known for its volatile oils, which are the main active components of A. argyi, including monoterpenes, sesquiterpenes and their derivatives. Despite its medicinal significance, the biosynthesis of sesquiterpenoids in A. argyi remains underexplored. In this study, we identified four β-caryophyllene synthases from A. argyi. A high-yield β-caryophyllene engineered Saccharomyces cerevisiae cell factory has been built in this study. By fusing ERG20 and AarTPS88 with a flexible linker (GGGS)2 and enhancing metabolic flux in the MVA pathway (HIF-1, tHMGR, and UPC2-1), we achieved a titer of β-caryophyllene reached 15.6 g/L by fed-batch fermentation in a 5 L bioreactor. To our knowledge, this represents the highest reported titer of β-caryophyllene in yeast to date. This study provides a valuable tool for the industrial-scale production of β-caryophyllene. |
| format | Article |
| id | doaj-art-83745ce9c0b149918841b2c8c9e3d152 |
| institution | DOAJ |
| issn | 2405-805X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj-art-83745ce9c0b149918841b2c8c9e3d1522025-08-20T03:13:21ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2025-03-0110115816410.1016/j.synbio.2024.09.005Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyiZhengping Li0Yuhong Gan1Changyu Gou2Qiongyu Ye3Yang Wu4Yuhong Wu5Tingxing Yang6Baolian Fan7Aijia Ji8Qi Shen9Lixin Duan10Guangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR ChinaGuangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR ChinaGuangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR ChinaGuangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR ChinaGuangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR ChinaGuangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR ChinaGuangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR ChinaGuangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR ChinaGuangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR ChinaInstitute of Medical Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR ChinaGuangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR China; Corresponding author.Artemisia argyi H. Lév. & Vaniot is an important traditional Chinese medicinal plant known for its volatile oils, which are the main active components of A. argyi, including monoterpenes, sesquiterpenes and their derivatives. Despite its medicinal significance, the biosynthesis of sesquiterpenoids in A. argyi remains underexplored. In this study, we identified four β-caryophyllene synthases from A. argyi. A high-yield β-caryophyllene engineered Saccharomyces cerevisiae cell factory has been built in this study. By fusing ERG20 and AarTPS88 with a flexible linker (GGGS)2 and enhancing metabolic flux in the MVA pathway (HIF-1, tHMGR, and UPC2-1), we achieved a titer of β-caryophyllene reached 15.6 g/L by fed-batch fermentation in a 5 L bioreactor. To our knowledge, this represents the highest reported titer of β-caryophyllene in yeast to date. This study provides a valuable tool for the industrial-scale production of β-caryophyllene.http://www.sciencedirect.com/science/article/pii/S2405805X24001248Artemisia argyiβ-caryophylleneβ-caryophyllene synthaseBiosynthesisSaccharomyces cerevisiae |
| spellingShingle | Zhengping Li Yuhong Gan Changyu Gou Qiongyu Ye Yang Wu Yuhong Wu Tingxing Yang Baolian Fan Aijia Ji Qi Shen Lixin Duan Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi Synthetic and Systems Biotechnology Artemisia argyi β-caryophyllene β-caryophyllene synthase Biosynthesis Saccharomyces cerevisiae |
| title | Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi |
| title_full | Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi |
| title_fullStr | Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi |
| title_full_unstemmed | Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi |
| title_short | Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi |
| title_sort | efficient biosynthesis of β caryophyllene in saccharomyces cerevisiae by β caryophyllene synthase from artemisia argyi |
| topic | Artemisia argyi β-caryophyllene β-caryophyllene synthase Biosynthesis Saccharomyces cerevisiae |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X24001248 |
| work_keys_str_mv | AT zhengpingli efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi AT yuhonggan efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi AT changyugou efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi AT qiongyuye efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi AT yangwu efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi AT yuhongwu efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi AT tingxingyang efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi AT baolianfan efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi AT aijiaji efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi AT qishen efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi AT lixinduan efficientbiosynthesisofbcaryophylleneinsaccharomycescerevisiaebybcaryophyllenesynthasefromartemisiaargyi |