Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS
The accurate determination of magnesium isotope values of chlorophyll a and chlorophyll b is particularly important for the study of magnesium biosynthesis pathways during chlorophyll formation. HPLC is required before MC-ICP-MS can be utilized, but HPLC lacks a method for separating chlorophylls. T...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Science Press, PR China
2024-05-01
|
| Series: | Yankuang ceshi |
| Subjects: | |
| Online Access: | http://www.ykcs.ac.cn/article/doi/10.15898/j.ykcs.202208300161 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850235732938981376 |
|---|---|
| author | Zihan AN Hongyu ZHANG Jiaxin YIN Pan ZHANG Min YAO Kangjun HUANG |
| author_facet | Zihan AN Hongyu ZHANG Jiaxin YIN Pan ZHANG Min YAO Kangjun HUANG |
| author_sort | Zihan AN |
| collection | DOAJ |
| description | The accurate determination of magnesium isotope values of chlorophyll a and chlorophyll b is particularly important for the study of magnesium biosynthesis pathways during chlorophyll formation. HPLC is required before MC-ICP-MS can be utilized, but HPLC lacks a method for separating chlorophylls. Therefore, it is necessary to develop a set of methods for separating plant chlorophyll a and chlorophyll b. The samples separated by this method are suitable for magnesium isotope determination by MC-ICP-MS. Based on the detection wavelength of 665nm and C18 column (7.6mm×250mm, 5μm), the HPLC conditions were optimized by three-factor and three-level orthogonal design to obtain the required parameters. The correlation coefficients of chlorophyll a and chlorophyll b standard curves in the concentration range of 5−50mg/L were greater than 0.9996, the detection limits were 0.40−1.09mg/L, the quantification limits were 1.22−3.31mg/L, the relative standard deviation was less than 8.10%, and the recoveries were 91.92%−111.11%. The magnesium isotope data of the isolated samples also showed that the column temperature of 25℃, flow rate of 1mL/min, and methanol-acetone (80∶20, V/V) mobile phase were reliable as HPLC separation conditions. The method established in this paper provides technical support for the separation of chlorophyll a and chlorophyll b in plants, and the separated samples can be used for magnesium isotope determination. The BRIEF REPORT is available for this paper at http://www.ykcs.ac.cn/en/article/doi/10.15898/j.ykcs.202208300161. |
| format | Article |
| id | doaj-art-8367141ba5b044188287655ec60dabf6 |
| institution | OA Journals |
| issn | 0254-5357 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Science Press, PR China |
| record_format | Article |
| series | Yankuang ceshi |
| spelling | doaj-art-8367141ba5b044188287655ec60dabf62025-08-20T02:02:09ZengScience Press, PR ChinaYankuang ceshi0254-53572024-05-0143347648610.15898/j.ykcs.202208300161yk202208300161Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MSZihan AN0Hongyu ZHANG1Jiaxin YIN2Pan ZHANG3Min YAO4Kangjun HUANG5State Key Laboratory of Continental Dynamics, Department of Geology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Continental Dynamics, Department of Geology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Continental Dynamics, Department of Geology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Continental Dynamics, Department of Geology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Continental Dynamics, Department of Geology, Northwest University, Xi’an 710069, ChinaState Key Laboratory of Continental Dynamics, Department of Geology, Northwest University, Xi’an 710069, ChinaThe accurate determination of magnesium isotope values of chlorophyll a and chlorophyll b is particularly important for the study of magnesium biosynthesis pathways during chlorophyll formation. HPLC is required before MC-ICP-MS can be utilized, but HPLC lacks a method for separating chlorophylls. Therefore, it is necessary to develop a set of methods for separating plant chlorophyll a and chlorophyll b. The samples separated by this method are suitable for magnesium isotope determination by MC-ICP-MS. Based on the detection wavelength of 665nm and C18 column (7.6mm×250mm, 5μm), the HPLC conditions were optimized by three-factor and three-level orthogonal design to obtain the required parameters. The correlation coefficients of chlorophyll a and chlorophyll b standard curves in the concentration range of 5−50mg/L were greater than 0.9996, the detection limits were 0.40−1.09mg/L, the quantification limits were 1.22−3.31mg/L, the relative standard deviation was less than 8.10%, and the recoveries were 91.92%−111.11%. The magnesium isotope data of the isolated samples also showed that the column temperature of 25℃, flow rate of 1mL/min, and methanol-acetone (80∶20, V/V) mobile phase were reliable as HPLC separation conditions. The method established in this paper provides technical support for the separation of chlorophyll a and chlorophyll b in plants, and the separated samples can be used for magnesium isotope determination. The BRIEF REPORT is available for this paper at http://www.ykcs.ac.cn/en/article/doi/10.15898/j.ykcs.202208300161.http://www.ykcs.ac.cn/article/doi/10.15898/j.ykcs.202208300161magnesium isotopestandard-sample-bracketing methodhigh performance liquid chromatographymobile phasechlorophyll achlorophyll b |
| spellingShingle | Zihan AN Hongyu ZHANG Jiaxin YIN Pan ZHANG Min YAO Kangjun HUANG Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS Yankuang ceshi magnesium isotope standard-sample-bracketing method high performance liquid chromatography mobile phase chlorophyll a chlorophyll b |
| title | Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS |
| title_full | Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS |
| title_fullStr | Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS |
| title_full_unstemmed | Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS |
| title_short | Determination of Magnesium Isotopic Composition of Plant Chlorophylls Based on HPLC and MC-ICP-MS |
| title_sort | determination of magnesium isotopic composition of plant chlorophylls based on hplc and mc icp ms |
| topic | magnesium isotope standard-sample-bracketing method high performance liquid chromatography mobile phase chlorophyll a chlorophyll b |
| url | http://www.ykcs.ac.cn/article/doi/10.15898/j.ykcs.202208300161 |
| work_keys_str_mv | AT zihanan determinationofmagnesiumisotopiccompositionofplantchlorophyllsbasedonhplcandmcicpms AT hongyuzhang determinationofmagnesiumisotopiccompositionofplantchlorophyllsbasedonhplcandmcicpms AT jiaxinyin determinationofmagnesiumisotopiccompositionofplantchlorophyllsbasedonhplcandmcicpms AT panzhang determinationofmagnesiumisotopiccompositionofplantchlorophyllsbasedonhplcandmcicpms AT minyao determinationofmagnesiumisotopiccompositionofplantchlorophyllsbasedonhplcandmcicpms AT kangjunhuang determinationofmagnesiumisotopiccompositionofplantchlorophyllsbasedonhplcandmcicpms |