Comparative Analysis of the Mitochondrial Genomes of Five Species of <i>Anabropsis</i> (Orthoptera: Anostostomatidae) and the Phylogenetic Implications of Anostostomatidae
In China, Anostostomatidae is represented by a single tribe, Anabropsini; two genera; and 33 species. Although extensive research has been conducted on Anabropsini, the monophyly of this tribe within Anostostomatidae remains unverified. Furthermore, the phylogenetic relationships within <i>Ana...
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2025-06-01
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| author | Tingting Yu Siyu Pang Wenjing Wang Ting Luo Yanting Qin Xun Bian Bin Zhang |
| author_facet | Tingting Yu Siyu Pang Wenjing Wang Ting Luo Yanting Qin Xun Bian Bin Zhang |
| author_sort | Tingting Yu |
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| description | In China, Anostostomatidae is represented by a single tribe, Anabropsini; two genera; and 33 species. Although extensive research has been conducted on Anabropsini, the monophyly of this tribe within Anostostomatidae remains unverified. Furthermore, the phylogenetic relationships within <i>Anabropsis</i> remain under debate. To address these gaps, we sequenced and annotated the mitochondrial genomes of five Anabropsini species to investigate their mitochondrial characteristics and phylogenetic positions and clarify the relationships among <i>Anabropsis</i> subgenera. The total mitochondrial length of the five species ranged from 15,985 bp to 16,423 bp and contained 13 protein-coding genes, 22 tRNAs, 2 rRNAs, and 1 control region. A grouped analysis of selection pressure on <i>Anabropsis</i> revealed that the Ka/Ks values for alate and apterous forms are not significantly different, suggesting that using wing length alone as the basis for dividing subgenera within <i>Anabropsis</i> may be unreliable. Tertiary structure modeling of proteins showed that the variable sites were concentrated in α-helix regions. Phylogenetic trees were reconstructed using the Bayesian inference and maximum likelihood methods and were based on two better datasets, namely, PCG123 (all codon positions of the PCGs) and PCG123 + 2R (all codon positions of PCGs, 12SrRNA, and 16SrRNA). The results indicate that the Chinese Anabropsini is paraphyletic, whereas <i>Anabropsis</i> is monophyletic, with a stable subgeneric topology. |
| format | Article |
| id | doaj-art-83663a2205f942bc98ce7732f1a617cc |
| institution | DOAJ |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-83663a2205f942bc98ce7732f1a617cc2025-08-20T03:13:41ZengMDPI AGBiology2079-77372025-06-0114777210.3390/biology14070772Comparative Analysis of the Mitochondrial Genomes of Five Species of <i>Anabropsis</i> (Orthoptera: Anostostomatidae) and the Phylogenetic Implications of AnostostomatidaeTingting Yu0Siyu Pang1Wenjing Wang2Ting Luo3Yanting Qin4Xun Bian5Bin Zhang6College of Life Sciences & Technology, Inner Mongolia Normal University, Hohhot 010022, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541006, ChinaCollege of Life Sciences & Technology, Inner Mongolia Normal University, Hohhot 010022, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541006, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541006, ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541006, ChinaCollege of Life Sciences & Technology, Inner Mongolia Normal University, Hohhot 010022, ChinaIn China, Anostostomatidae is represented by a single tribe, Anabropsini; two genera; and 33 species. Although extensive research has been conducted on Anabropsini, the monophyly of this tribe within Anostostomatidae remains unverified. Furthermore, the phylogenetic relationships within <i>Anabropsis</i> remain under debate. To address these gaps, we sequenced and annotated the mitochondrial genomes of five Anabropsini species to investigate their mitochondrial characteristics and phylogenetic positions and clarify the relationships among <i>Anabropsis</i> subgenera. The total mitochondrial length of the five species ranged from 15,985 bp to 16,423 bp and contained 13 protein-coding genes, 22 tRNAs, 2 rRNAs, and 1 control region. A grouped analysis of selection pressure on <i>Anabropsis</i> revealed that the Ka/Ks values for alate and apterous forms are not significantly different, suggesting that using wing length alone as the basis for dividing subgenera within <i>Anabropsis</i> may be unreliable. Tertiary structure modeling of proteins showed that the variable sites were concentrated in α-helix regions. Phylogenetic trees were reconstructed using the Bayesian inference and maximum likelihood methods and were based on two better datasets, namely, PCG123 (all codon positions of the PCGs) and PCG123 + 2R (all codon positions of PCGs, 12SrRNA, and 16SrRNA). The results indicate that the Chinese Anabropsini is paraphyletic, whereas <i>Anabropsis</i> is monophyletic, with a stable subgeneric topology.https://www.mdpi.com/2079-7737/14/7/772Anostostomatidaephylogenetic analysismitogenomicsAnabropsini |
| spellingShingle | Tingting Yu Siyu Pang Wenjing Wang Ting Luo Yanting Qin Xun Bian Bin Zhang Comparative Analysis of the Mitochondrial Genomes of Five Species of <i>Anabropsis</i> (Orthoptera: Anostostomatidae) and the Phylogenetic Implications of Anostostomatidae Biology Anostostomatidae phylogenetic analysis mitogenomics Anabropsini |
| title | Comparative Analysis of the Mitochondrial Genomes of Five Species of <i>Anabropsis</i> (Orthoptera: Anostostomatidae) and the Phylogenetic Implications of Anostostomatidae |
| title_full | Comparative Analysis of the Mitochondrial Genomes of Five Species of <i>Anabropsis</i> (Orthoptera: Anostostomatidae) and the Phylogenetic Implications of Anostostomatidae |
| title_fullStr | Comparative Analysis of the Mitochondrial Genomes of Five Species of <i>Anabropsis</i> (Orthoptera: Anostostomatidae) and the Phylogenetic Implications of Anostostomatidae |
| title_full_unstemmed | Comparative Analysis of the Mitochondrial Genomes of Five Species of <i>Anabropsis</i> (Orthoptera: Anostostomatidae) and the Phylogenetic Implications of Anostostomatidae |
| title_short | Comparative Analysis of the Mitochondrial Genomes of Five Species of <i>Anabropsis</i> (Orthoptera: Anostostomatidae) and the Phylogenetic Implications of Anostostomatidae |
| title_sort | comparative analysis of the mitochondrial genomes of five species of i anabropsis i orthoptera anostostomatidae and the phylogenetic implications of anostostomatidae |
| topic | Anostostomatidae phylogenetic analysis mitogenomics Anabropsini |
| url | https://www.mdpi.com/2079-7737/14/7/772 |
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