Chronic heat stress facilitates lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broiler chickens

Abstract Exposure to chronic heat stress not only reduces body weight gain in growing broilers, but also enhances fat deposition. To elucidate this effect, numerous studies have investigated the changes in the liver, the primary site of de novo fat synthesis in avian species, and a marked increase i...

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Main Authors: Yukako Tokutake, Takashi Miyazaki, Chinatsu Abe, Md. Rezwanul Habib, Shinichi Yonekura, Kan Sato
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-025-03439-0
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author Yukako Tokutake
Takashi Miyazaki
Chinatsu Abe
Md. Rezwanul Habib
Shinichi Yonekura
Kan Sato
author_facet Yukako Tokutake
Takashi Miyazaki
Chinatsu Abe
Md. Rezwanul Habib
Shinichi Yonekura
Kan Sato
author_sort Yukako Tokutake
collection DOAJ
description Abstract Exposure to chronic heat stress not only reduces body weight gain in growing broilers, but also enhances fat deposition. To elucidate this effect, numerous studies have investigated the changes in the liver, the primary site of de novo fat synthesis in avian species, and a marked increase in fat accumulation has been observed under chronic heat stress. However, the impact of heat stress on adipose tissue remains largely unclear. To investigate alterations in adipose tissue following heat-exposure, we reared growing broilers in a high-temperature environment (32 °C) for 14 d. Despite the decreased feed intake, the ratio of abdominal fat to body weight in the heat-exposed group remained comparable to that in the control group. Furthermore, the heat-exposed group exhibited a marked decrease in plasma free fatty acid concentrations. Transcriptome analysis of abdominal fat from heat-exposed and control groups indicated 459 differentially expressed genes. In the heat-exposed group, genes associated with lipid synthesis showed increased expression in the adipose tissue. Heat stimulation induced the expression of the lipoprotein lipase gene in cultured primary adipocytes. These results suggest that chronic heat exposure promotes lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broilers.
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issn 2045-2322
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spelling doaj-art-835a0bf84fee4422813d78c4f35b2b1a2025-08-20T03:16:31ZengNature PortfolioScientific Reports2045-23222025-05-0115111310.1038/s41598-025-03439-0Chronic heat stress facilitates lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broiler chickensYukako Tokutake0Takashi Miyazaki1Chinatsu Abe2Md. Rezwanul Habib3Shinichi Yonekura4Kan Sato5Graduate School of Science and Technology, Shinshu UniversityGraduate School of Science and Technology, Shinshu UniversityFaculty of Agriculture, Shinshu UniversityGraduate School of Science and Technology, Shinshu UniversityGraduate School of Science and Technology, Shinshu UniversityGraduate School of Agricultural Science, Tohoku UniversityAbstract Exposure to chronic heat stress not only reduces body weight gain in growing broilers, but also enhances fat deposition. To elucidate this effect, numerous studies have investigated the changes in the liver, the primary site of de novo fat synthesis in avian species, and a marked increase in fat accumulation has been observed under chronic heat stress. However, the impact of heat stress on adipose tissue remains largely unclear. To investigate alterations in adipose tissue following heat-exposure, we reared growing broilers in a high-temperature environment (32 °C) for 14 d. Despite the decreased feed intake, the ratio of abdominal fat to body weight in the heat-exposed group remained comparable to that in the control group. Furthermore, the heat-exposed group exhibited a marked decrease in plasma free fatty acid concentrations. Transcriptome analysis of abdominal fat from heat-exposed and control groups indicated 459 differentially expressed genes. In the heat-exposed group, genes associated with lipid synthesis showed increased expression in the adipose tissue. Heat stimulation induced the expression of the lipoprotein lipase gene in cultured primary adipocytes. These results suggest that chronic heat exposure promotes lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broilers.https://doi.org/10.1038/s41598-025-03439-0BroilerChronic heat stressAdipose tissueLipoprotein lipase
spellingShingle Yukako Tokutake
Takashi Miyazaki
Chinatsu Abe
Md. Rezwanul Habib
Shinichi Yonekura
Kan Sato
Chronic heat stress facilitates lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broiler chickens
Scientific Reports
Broiler
Chronic heat stress
Adipose tissue
Lipoprotein lipase
title Chronic heat stress facilitates lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broiler chickens
title_full Chronic heat stress facilitates lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broiler chickens
title_fullStr Chronic heat stress facilitates lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broiler chickens
title_full_unstemmed Chronic heat stress facilitates lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broiler chickens
title_short Chronic heat stress facilitates lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broiler chickens
title_sort chronic heat stress facilitates lipoprotein lipase expression and triglyceride biosynthesis in the adipose tissue of growing broiler chickens
topic Broiler
Chronic heat stress
Adipose tissue
Lipoprotein lipase
url https://doi.org/10.1038/s41598-025-03439-0
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