Circ_RPPH1 promotes bladder urothelium carcinoma proliferation and EMT by recruiting and binding to EIF4 A3
Abstract Background The involvement of circ_RPPH1 in bladder urothelial carcinoma (BUC) remains unclear, as well as the underlying mechanism. Methods Circ_RPPH1 levels in BUC cells and tissues were measured via RT-qPCR. Downregulation of circ_RPPH1 was assessed using colony formation, CCK-8, wound h...
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BMC
2025-05-01
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| Series: | Hereditas |
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| Online Access: | https://doi.org/10.1186/s41065-025-00442-3 |
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| author | HuaWei Liu JunMin Ma Xia Yan |
| author_facet | HuaWei Liu JunMin Ma Xia Yan |
| author_sort | HuaWei Liu |
| collection | DOAJ |
| description | Abstract Background The involvement of circ_RPPH1 in bladder urothelial carcinoma (BUC) remains unclear, as well as the underlying mechanism. Methods Circ_RPPH1 levels in BUC cells and tissues were measured via RT-qPCR. Downregulation of circ_RPPH1 was assessed using colony formation, CCK-8, wound healing, and Transwell assays to evaluate proliferation, migration, and invasion. RIP and RNA pull-down confirmed circ_RPPH1 binding to EIF4A3, while immunoblotting analyzed EIF4A3 and EMT-related proteins. Results High circ_RPPH1 levels in BUC correlated with tumor invasion depth. Its knockout suppressed proliferation, invasion, and EMT, while circ_RPPH1 overexpression reduced EIF4A3 binding to N-cadherin and Vimentin mRNA, promoting EMT. Conclusion Circ_RPPH1 promotes tumor growth and EMT in BUC by inhibiting EIF4A3-mediated mRNA regulation, activating the EIF4A3/N-cadherin/Vimentin pathway. |
| format | Article |
| id | doaj-art-833e4e9c45ef4c55b744eeafd0cb63f8 |
| institution | DOAJ |
| issn | 1601-5223 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj-art-833e4e9c45ef4c55b744eeafd0cb63f82025-08-20T03:09:20ZengBMCHereditas1601-52232025-05-0116211910.1186/s41065-025-00442-3Circ_RPPH1 promotes bladder urothelium carcinoma proliferation and EMT by recruiting and binding to EIF4 A3HuaWei Liu0JunMin Ma1Xia Yan2Department of Urology, Deyang People’s Hospital, Sichuan ProvinceDepartment of Urology, Huaian Hospital of Huaian CityDepartment of Inspection Division, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Hubei ProvinceAbstract Background The involvement of circ_RPPH1 in bladder urothelial carcinoma (BUC) remains unclear, as well as the underlying mechanism. Methods Circ_RPPH1 levels in BUC cells and tissues were measured via RT-qPCR. Downregulation of circ_RPPH1 was assessed using colony formation, CCK-8, wound healing, and Transwell assays to evaluate proliferation, migration, and invasion. RIP and RNA pull-down confirmed circ_RPPH1 binding to EIF4A3, while immunoblotting analyzed EIF4A3 and EMT-related proteins. Results High circ_RPPH1 levels in BUC correlated with tumor invasion depth. Its knockout suppressed proliferation, invasion, and EMT, while circ_RPPH1 overexpression reduced EIF4A3 binding to N-cadherin and Vimentin mRNA, promoting EMT. Conclusion Circ_RPPH1 promotes tumor growth and EMT in BUC by inhibiting EIF4A3-mediated mRNA regulation, activating the EIF4A3/N-cadherin/Vimentin pathway.https://doi.org/10.1186/s41065-025-00442-3Circ_RPPH1EIF4 A3Bladder cancerEMT |
| spellingShingle | HuaWei Liu JunMin Ma Xia Yan Circ_RPPH1 promotes bladder urothelium carcinoma proliferation and EMT by recruiting and binding to EIF4 A3 Hereditas Circ_RPPH1 EIF4 A3 Bladder cancer EMT |
| title | Circ_RPPH1 promotes bladder urothelium carcinoma proliferation and EMT by recruiting and binding to EIF4 A3 |
| title_full | Circ_RPPH1 promotes bladder urothelium carcinoma proliferation and EMT by recruiting and binding to EIF4 A3 |
| title_fullStr | Circ_RPPH1 promotes bladder urothelium carcinoma proliferation and EMT by recruiting and binding to EIF4 A3 |
| title_full_unstemmed | Circ_RPPH1 promotes bladder urothelium carcinoma proliferation and EMT by recruiting and binding to EIF4 A3 |
| title_short | Circ_RPPH1 promotes bladder urothelium carcinoma proliferation and EMT by recruiting and binding to EIF4 A3 |
| title_sort | circ rpph1 promotes bladder urothelium carcinoma proliferation and emt by recruiting and binding to eif4 a3 |
| topic | Circ_RPPH1 EIF4 A3 Bladder cancer EMT |
| url | https://doi.org/10.1186/s41065-025-00442-3 |
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