Identification of SSR Markers for Differentiating Rice (Oryza sativa L.) Varieties Marketed in Turkey

This study was carried out to identify SSR markers for the differentiation and identification of rice cultivars marketed in Turkey between 2016 and 2017. In this study 60 registered orproduction permitted, some local and foreign rice varieties were used as a material. DNA was isolated from single po...

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Main Authors: NECMI Beser, ZEYNEP ÇİSEM Mutafçılar
Format: Article
Language:English
Published: Ankara University 2020-09-01
Series:Journal of Agricultural Sciences
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Online Access:https://dergipark.org.tr/tr/download/article-file/1262517
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author NECMI Beser
ZEYNEP ÇİSEM Mutafçılar
author_facet NECMI Beser
ZEYNEP ÇİSEM Mutafçılar
author_sort NECMI Beser
collection DOAJ
description This study was carried out to identify SSR markers for the differentiation and identification of rice cultivars marketed in Turkey between 2016 and 2017. In this study 60 registered orproduction permitted, some local and foreign rice varieties were used as a material. DNA was isolated from single polished rice kernels for PCR amplification and rice cultivars were genotyped by 50 SSR markers. We found that 45 out of 50 SSR markers produced reproducible and polymorphic alleles. Thirty-six rice varieties had variety specific alleles among 60 rice varieties analysed in the study. This variety specific alleles belong to only one variety and they can be used to identify rice variety among 60 rice varieties studied. Osmancık-97, Cammeo, Ronaldo and Baldo are the most import 4 varieties in Turkish rice production and milled rice market. In this study, it was found that RM152, RM144, RM259 and RM118 SSR markers can be used to differentiate and identify these most important four varieties with different combinations. Collectively, this study provided some variety specific SSR markers that can be used to differentiate and identify rice varieties sold in Turkey.
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publishDate 2020-09-01
publisher Ankara University
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series Journal of Agricultural Sciences
spelling doaj-art-83294c2fc2e44864a6d9be57db0986c72025-08-20T03:07:13ZengAnkara UniversityJournal of Agricultural Sciences1300-75802148-92972020-09-0126335736210.15832/ankutbd.51827645Identification of SSR Markers for Differentiating Rice (Oryza sativa L.) Varieties Marketed in TurkeyNECMI Beser0ZEYNEP ÇİSEM Mutafçılar1TRAKYA ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ, GENETİK VE BİYOMÜHENDİSLİK BÖLÜMÜTRAKYA ÜNİVERSİTESİThis study was carried out to identify SSR markers for the differentiation and identification of rice cultivars marketed in Turkey between 2016 and 2017. In this study 60 registered orproduction permitted, some local and foreign rice varieties were used as a material. DNA was isolated from single polished rice kernels for PCR amplification and rice cultivars were genotyped by 50 SSR markers. We found that 45 out of 50 SSR markers produced reproducible and polymorphic alleles. Thirty-six rice varieties had variety specific alleles among 60 rice varieties analysed in the study. This variety specific alleles belong to only one variety and they can be used to identify rice variety among 60 rice varieties studied. Osmancık-97, Cammeo, Ronaldo and Baldo are the most import 4 varieties in Turkish rice production and milled rice market. In this study, it was found that RM152, RM144, RM259 and RM118 SSR markers can be used to differentiate and identify these most important four varieties with different combinations. Collectively, this study provided some variety specific SSR markers that can be used to differentiate and identify rice varieties sold in Turkey.https://dergipark.org.tr/tr/download/article-file/1262517polished ricerice cultivarssr
spellingShingle NECMI Beser
ZEYNEP ÇİSEM Mutafçılar
Identification of SSR Markers for Differentiating Rice (Oryza sativa L.) Varieties Marketed in Turkey
Journal of Agricultural Sciences
polished rice
rice cultivar
ssr
title Identification of SSR Markers for Differentiating Rice (Oryza sativa L.) Varieties Marketed in Turkey
title_full Identification of SSR Markers for Differentiating Rice (Oryza sativa L.) Varieties Marketed in Turkey
title_fullStr Identification of SSR Markers for Differentiating Rice (Oryza sativa L.) Varieties Marketed in Turkey
title_full_unstemmed Identification of SSR Markers for Differentiating Rice (Oryza sativa L.) Varieties Marketed in Turkey
title_short Identification of SSR Markers for Differentiating Rice (Oryza sativa L.) Varieties Marketed in Turkey
title_sort identification of ssr markers for differentiating rice oryza sativa l varieties marketed in turkey
topic polished rice
rice cultivar
ssr
url https://dergipark.org.tr/tr/download/article-file/1262517
work_keys_str_mv AT necmibeser identificationofssrmarkersfordifferentiatingriceoryzasativalvarietiesmarketedinturkey
AT zeynepcisemmutafcılar identificationofssrmarkersfordifferentiatingriceoryzasativalvarietiesmarketedinturkey