Gene Expression Profiling of the Optic Nerve Head of Patients with Primary Open-Angle Glaucoma
Background. The pressure-induced axonal injury of the vulnerable ONH has led many researchers to view glaucoma from the perspective of the genetic basis of the angle of the ONH. However, genetic studies on POAG from this perspective are limited. Methods. Microarray dataset GSE45570 of the ONH of hea...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2017/6896390 |
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| author | Xinrong Wang Ke Gong Haiyan Li Congyi Wang Chaoyi Qu Hui Li |
| author_facet | Xinrong Wang Ke Gong Haiyan Li Congyi Wang Chaoyi Qu Hui Li |
| author_sort | Xinrong Wang |
| collection | DOAJ |
| description | Background. The pressure-induced axonal injury of the vulnerable ONH has led many researchers to view glaucoma from the perspective of the genetic basis of the angle of the ONH. However, genetic studies on POAG from this perspective are limited. Methods. Microarray dataset GSE45570 of the ONH of healthy individuals and POAG patients were downloaded from the Gene Expression Omnibus. After screening for the DEGs using the limma package, enrichment analysis was performed using DAVID. The DEG interaction network was constructed using cancer spider at BioProfiling.de. Thereafter, DEG-related TFs were predicted using TRANSFAC, and TF-DEG regulatory networks were visualized using Cytoscape. Results. Thirty-one DEGs were identified including 11 upregulated and 20 downregulated DEGs. Thereafter, gene ontology terms of nucleosome assembly, sensory perception and cognition, and pathway of signaling by GPCR were found to be enriched among the DEGs. Furthermore, DEG interaction and TF-DEG networks were constructed. NEUROD1 was present in both the DEG network and the TF-DEG network as the node with the highest degree and was predicted as a marker gene in the ONH of patients with POAG. Conclusion. NEUROD1 may contribute greatly to the ONH of patients with POAG and was found to be involved in eye development and diseases. |
| format | Article |
| id | doaj-art-832427b4cd974f65b9760596abca41f7 |
| institution | OA Journals |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-832427b4cd974f65b9760596abca41f72025-08-20T02:09:00ZengWileyJournal of Ophthalmology2090-004X2090-00582017-01-01201710.1155/2017/68963906896390Gene Expression Profiling of the Optic Nerve Head of Patients with Primary Open-Angle GlaucomaXinrong Wang0Ke Gong1Haiyan Li2Congyi Wang3Chaoyi Qu4Hui Li5Department of Ophthalmology, The Fourth Hospital of Xi’an, Xi’an, Shaanxi 710004, ChinaDepartment of Ophthalmology, The Fourth Hospital of Xi’an, Xi’an, Shaanxi 710004, ChinaDepartment of Ophthalmology, The Fourth Hospital of Xi’an, Xi’an, Shaanxi 710004, ChinaDepartment of Ophthalmology, The Fourth Hospital of Xi’an, Xi’an, Shaanxi 710004, ChinaDepartment of Ophthalmology, The Fourth Hospital of Xi’an, Xi’an, Shaanxi 710004, ChinaDepartment of Endocrinology, Shaanxi Provincial People’s Hospital, Xi’an, Shaanxi 710068, ChinaBackground. The pressure-induced axonal injury of the vulnerable ONH has led many researchers to view glaucoma from the perspective of the genetic basis of the angle of the ONH. However, genetic studies on POAG from this perspective are limited. Methods. Microarray dataset GSE45570 of the ONH of healthy individuals and POAG patients were downloaded from the Gene Expression Omnibus. After screening for the DEGs using the limma package, enrichment analysis was performed using DAVID. The DEG interaction network was constructed using cancer spider at BioProfiling.de. Thereafter, DEG-related TFs were predicted using TRANSFAC, and TF-DEG regulatory networks were visualized using Cytoscape. Results. Thirty-one DEGs were identified including 11 upregulated and 20 downregulated DEGs. Thereafter, gene ontology terms of nucleosome assembly, sensory perception and cognition, and pathway of signaling by GPCR were found to be enriched among the DEGs. Furthermore, DEG interaction and TF-DEG networks were constructed. NEUROD1 was present in both the DEG network and the TF-DEG network as the node with the highest degree and was predicted as a marker gene in the ONH of patients with POAG. Conclusion. NEUROD1 may contribute greatly to the ONH of patients with POAG and was found to be involved in eye development and diseases.http://dx.doi.org/10.1155/2017/6896390 |
| spellingShingle | Xinrong Wang Ke Gong Haiyan Li Congyi Wang Chaoyi Qu Hui Li Gene Expression Profiling of the Optic Nerve Head of Patients with Primary Open-Angle Glaucoma Journal of Ophthalmology |
| title | Gene Expression Profiling of the Optic Nerve Head of Patients with Primary Open-Angle Glaucoma |
| title_full | Gene Expression Profiling of the Optic Nerve Head of Patients with Primary Open-Angle Glaucoma |
| title_fullStr | Gene Expression Profiling of the Optic Nerve Head of Patients with Primary Open-Angle Glaucoma |
| title_full_unstemmed | Gene Expression Profiling of the Optic Nerve Head of Patients with Primary Open-Angle Glaucoma |
| title_short | Gene Expression Profiling of the Optic Nerve Head of Patients with Primary Open-Angle Glaucoma |
| title_sort | gene expression profiling of the optic nerve head of patients with primary open angle glaucoma |
| url | http://dx.doi.org/10.1155/2017/6896390 |
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