Engineering bioactive mineralized tumor cells for tumor immunotherapy
IntroductionWhole-cell tumor vaccines are advantageous because of their ability to induce a broad and multifaceted immune response through the presentation of a wide range of tumor antigens, thereby enhancing the ability of the immune system to recognize and target cancerous cells.MethodIn this stud...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1582490/full |
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| Summary: | IntroductionWhole-cell tumor vaccines are advantageous because of their ability to induce a broad and multifaceted immune response through the presentation of a wide range of tumor antigens, thereby enhancing the ability of the immune system to recognize and target cancerous cells.MethodIn this study, we present a multifunctional vaccine that consists of manganese-mineralized tumor cells and positively charged polymer-immobilized CpG. The Mn2+ and CpG released from the engineered vaccine facilitate the maturation of dendritic cells through the activation of the cGAS-STING and TLR9 pathways, respectively.ResultAs a consequence, the engineered vaccine derived from B16F10 cells exhibited a pronounced tumor-suppressive effect, reducing the tumor volume to approximately one-fifth of that in the control group, and significantly extending survival to day 30 in B16F10 tumor-bearing mice. This superior therapeutic outcome is associated with enhanced activation of dendritic cells, increased infiltration of NK and CD8+ T cells, and increased production of immune cytokines within the tumor microenvironment.DiscussionTogether, our study highlights the immense potential of engineering bioactive mineralized tumor cells to facilitate whole-cell tumor vaccine-based immunotherapy. |
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| ISSN: | 2296-4185 |