The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes
MADS-box genes are classified into five categories: ABCDE, including SEP1, SEP2, SEP3, SEP4, and other homologous genes, which play important roles in floral organ development. In this study, the cDNA sequence of the HrSEP1 gene was cloned by RT-PCR and confirmed that this gene belongs to the MADS-b...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1503346/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832582908455419904 |
|---|---|
| author | Di Cong Xue Zhao Xue Zhao Chang Ni Mengru Li Luwen Han Jianlin Cheng Hongzhang Liu Huijing Liu Dan Yao Shuying Liu Guoshuang Chen |
| author_facet | Di Cong Xue Zhao Xue Zhao Chang Ni Mengru Li Luwen Han Jianlin Cheng Hongzhang Liu Huijing Liu Dan Yao Shuying Liu Guoshuang Chen |
| author_sort | Di Cong |
| collection | DOAJ |
| description | MADS-box genes are classified into five categories: ABCDE, including SEP1, SEP2, SEP3, SEP4, and other homologous genes, which play important roles in floral organ development. In this study, the cDNA sequence of the HrSEP1 gene was cloned by RT-PCR and confirmed that this gene belongs to the MADS-box gene family. In addition, subcellular localization experiments showed that the HrSEP1 protein was localized in the nucleus. We verified the interaction of HrSEP1 with HrSOC1, HrSVP, and HrAP1 using yeast two-hybrid and bimolecular fluorescence complementation assays. These genes jointly regulate the growth and development of floral organs. We also found a strong synergy between HrSEP1 and AP1 genes in sepals, petals, and stamens by transgenic methods and fluorescence quantitative PCR, suggesting that HrSEP1 and AP1 may co-regulate the development of these structures. In conclusion, the expression of HrSEP1 has a certain effect on the development of floral organs, and these findings lay the foundation for further research on the biological functions of MADS transcription factors in Hippophae rhamnoides. |
| format | Article |
| id | doaj-art-83052b1d5e8d465f85b59f4d571382aa |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-83052b1d5e8d465f85b59f4d571382aa2025-01-29T06:45:45ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15033461503346The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genesDi Cong0Xue Zhao1Xue Zhao2Chang Ni3Mengru Li4Luwen Han5Jianlin Cheng6Hongzhang Liu7Huijing Liu8Dan Yao9Shuying Liu10Guoshuang Chen11College of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaGuangyuan Food and Drug Inspection and Testing Centre, Sichuan, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaCollege of Life Sciences, Jilin Agricultural University, Changchun, ChinaNortheast Institute of Geography and Agroecology, Chinese Academy of Sciences, Beijing, ChinaMADS-box genes are classified into five categories: ABCDE, including SEP1, SEP2, SEP3, SEP4, and other homologous genes, which play important roles in floral organ development. In this study, the cDNA sequence of the HrSEP1 gene was cloned by RT-PCR and confirmed that this gene belongs to the MADS-box gene family. In addition, subcellular localization experiments showed that the HrSEP1 protein was localized in the nucleus. We verified the interaction of HrSEP1 with HrSOC1, HrSVP, and HrAP1 using yeast two-hybrid and bimolecular fluorescence complementation assays. These genes jointly regulate the growth and development of floral organs. We also found a strong synergy between HrSEP1 and AP1 genes in sepals, petals, and stamens by transgenic methods and fluorescence quantitative PCR, suggesting that HrSEP1 and AP1 may co-regulate the development of these structures. In conclusion, the expression of HrSEP1 has a certain effect on the development of floral organs, and these findings lay the foundation for further research on the biological functions of MADS transcription factors in Hippophae rhamnoides.https://www.frontiersin.org/articles/10.3389/fpls.2024.1503346/fullHippophae rhamnoidesMADS-box genesyeast two-hybridbimolecular fluorescence complementationgenetic transformation |
| spellingShingle | Di Cong Xue Zhao Xue Zhao Chang Ni Mengru Li Luwen Han Jianlin Cheng Hongzhang Liu Huijing Liu Dan Yao Shuying Liu Guoshuang Chen The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes Frontiers in Plant Science Hippophae rhamnoides MADS-box genes yeast two-hybrid bimolecular fluorescence complementation genetic transformation |
| title | The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes |
| title_full | The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes |
| title_fullStr | The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes |
| title_full_unstemmed | The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes |
| title_short | The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes |
| title_sort | sepallata like gene hrsep1 in hippophae rhamnoides regulates flower development by interacting with other mads box subfamily genes |
| topic | Hippophae rhamnoides MADS-box genes yeast two-hybrid bimolecular fluorescence complementation genetic transformation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1503346/full |
| work_keys_str_mv | AT dicong thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT xuezhao thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT xuezhao thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT changni thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT mengruli thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT luwenhan thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT jianlincheng thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT hongzhangliu thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT huijingliu thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT danyao thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT shuyingliu thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT guoshuangchen thesepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT dicong sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT xuezhao sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT xuezhao sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT changni sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT mengruli sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT luwenhan sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT jianlincheng sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT hongzhangliu sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT huijingliu sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT danyao sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT shuyingliu sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes AT guoshuangchen sepallatalikegenehrsep1inhippophaerhamnoidesregulatesflowerdevelopmentbyinteractingwithothermadsboxsubfamilygenes |