Effect of smear layer removal agents on the microhardness and roughness of radicular dentin

Purpose: To evaluate the effect of phytic acid (IP6) on the surface roughness and microhardness of human root canal dentin and compare it to other smear layer removal agents. Materials and methods: Fifty extracted human maxillary incisors were sectioned longitudinally into a total of 100 specimens f...

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Main Authors: Hosea Lalrin Muana, Mohannad Nassar, Ahmad Dargham, Noriko Hiraishi, Junji Tagami
Format: Article
Language:English
Published: Springer 2021-11-01
Series:Saudi Dental Journal
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S101390522030273X
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author Hosea Lalrin Muana
Mohannad Nassar
Ahmad Dargham
Noriko Hiraishi
Junji Tagami
author_facet Hosea Lalrin Muana
Mohannad Nassar
Ahmad Dargham
Noriko Hiraishi
Junji Tagami
author_sort Hosea Lalrin Muana
collection DOAJ
description Purpose: To evaluate the effect of phytic acid (IP6) on the surface roughness and microhardness of human root canal dentin and compare it to other smear layer removal agents. Materials and methods: Fifty extracted human maxillary incisors were sectioned longitudinally into a total of 100 specimens followed by embedding in auto-polymerizing acrylic resin. The specimens were polished and then randomly divided into five groups (n = 20) according to the test solution used to condition root canal dentin: 17% ethylenediaminetetraacetic acid (EDTA); 10% citric acid (CA); 1% IP6; 37% phosphoric acid (PA); or distilled water (control group). Each specimen was treated with a total volume of 1 ml of each solution for 1 min with agitation. Each group was then divided into two subgroups of 10 specimens each. The specimens of the first subgroup were used to determine microhardness, using Vickers hardness tester, and the specimens of the second subgroup were used to measure surface roughness, using a confocal laser scanning microscope. The results were analyzed statistically using one-way ANOVA and Tukey tests, α = 0.05. Results: All the tested groups exhibited microhardness and surface roughness values that were statistically significantly different when compared with the control group (P < 0.05). The microhardness value obtained with IP6 was significantly lower when compared to EDTA, CA, and the control group, whereas its roughness value was significantly higher compared to the aforementioned groups. However, there was no significant difference between IP6 and PA (P > 0.05). Conclusions: IP6 and PA showed the lowest microhardness and the highest surface roughness values.
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spelling doaj-art-83021a10913f4294a4b5b6f7da84dff52025-08-20T02:15:54ZengSpringerSaudi Dental Journal1013-90522021-11-0133766166510.1016/j.sdentj.2020.05.001Effect of smear layer removal agents on the microhardness and roughness of radicular dentinHosea Lalrin Muana0Mohannad Nassar1Ahmad Dargham2Noriko Hiraishi3Junji Tagami4Department of Cariology and Operative Dentistry, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Preventive and Restorative Dentistry, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates; Corresponding author.RAK College of Dental Sciences (RAKCODS), RAK Medical and Health Sciences University, Ras Al-Khaimah, United Arab EmiratesDepartment of Cariology and Operative Dentistry, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, JapanDepartment of Cariology and Operative Dentistry, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, JapanPurpose: To evaluate the effect of phytic acid (IP6) on the surface roughness and microhardness of human root canal dentin and compare it to other smear layer removal agents. Materials and methods: Fifty extracted human maxillary incisors were sectioned longitudinally into a total of 100 specimens followed by embedding in auto-polymerizing acrylic resin. The specimens were polished and then randomly divided into five groups (n = 20) according to the test solution used to condition root canal dentin: 17% ethylenediaminetetraacetic acid (EDTA); 10% citric acid (CA); 1% IP6; 37% phosphoric acid (PA); or distilled water (control group). Each specimen was treated with a total volume of 1 ml of each solution for 1 min with agitation. Each group was then divided into two subgroups of 10 specimens each. The specimens of the first subgroup were used to determine microhardness, using Vickers hardness tester, and the specimens of the second subgroup were used to measure surface roughness, using a confocal laser scanning microscope. The results were analyzed statistically using one-way ANOVA and Tukey tests, α = 0.05. Results: All the tested groups exhibited microhardness and surface roughness values that were statistically significantly different when compared with the control group (P < 0.05). The microhardness value obtained with IP6 was significantly lower when compared to EDTA, CA, and the control group, whereas its roughness value was significantly higher compared to the aforementioned groups. However, there was no significant difference between IP6 and PA (P > 0.05). Conclusions: IP6 and PA showed the lowest microhardness and the highest surface roughness values.http://www.sciencedirect.com/science/article/pii/S101390522030273XCitric acidEDTAMicrohardnessPhosphoric acidPhytic acidRoughness
spellingShingle Hosea Lalrin Muana
Mohannad Nassar
Ahmad Dargham
Noriko Hiraishi
Junji Tagami
Effect of smear layer removal agents on the microhardness and roughness of radicular dentin
Saudi Dental Journal
Citric acid
EDTA
Microhardness
Phosphoric acid
Phytic acid
Roughness
title Effect of smear layer removal agents on the microhardness and roughness of radicular dentin
title_full Effect of smear layer removal agents on the microhardness and roughness of radicular dentin
title_fullStr Effect of smear layer removal agents on the microhardness and roughness of radicular dentin
title_full_unstemmed Effect of smear layer removal agents on the microhardness and roughness of radicular dentin
title_short Effect of smear layer removal agents on the microhardness and roughness of radicular dentin
title_sort effect of smear layer removal agents on the microhardness and roughness of radicular dentin
topic Citric acid
EDTA
Microhardness
Phosphoric acid
Phytic acid
Roughness
url http://www.sciencedirect.com/science/article/pii/S101390522030273X
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