Characterization of the glutathione redox state in the Golgi apparatus
Redox homeostasis is crucial for cell function, and, in eukaryotic cells, studying it in a compartmentalized way is essential due to the redox variations between different organelles. The redox state of organelles is largely determined by the redox potential of glutathione, EGSH, and the concentrati...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
|
| Series: | Redox Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231725000734 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850219723119132672 |
|---|---|
| author | Carla Miró-Vinyals Sarah Emmert Gina Grammbitter Alex Jud Tobias Kockmann Pablo Rivera-Fuentes |
| author_facet | Carla Miró-Vinyals Sarah Emmert Gina Grammbitter Alex Jud Tobias Kockmann Pablo Rivera-Fuentes |
| author_sort | Carla Miró-Vinyals |
| collection | DOAJ |
| description | Redox homeostasis is crucial for cell function, and, in eukaryotic cells, studying it in a compartmentalized way is essential due to the redox variations between different organelles. The redox state of organelles is largely determined by the redox potential of glutathione, EGSH, and the concentration of its reduced and oxidized species, [GS]. The Golgi apparatus is an essential component of the secretory pathway, yet little is known about the concentration or redox state of GSH in this organelle. Here, we characterized the redox state of GSH in the Golgi apparatus using a combination of microscopy and proteomics methods. Our results prove that the Golgi apparatus is a highly oxidizing organelle with a strikingly low GSH concentration (EGSH = – 157 mV, 1–5 mM). These results fill an important gap in our knowledge of redox homeostasis in subcellular organelles. Moreover, the new Golgi-targeted GSH sensors allow us to observe dynamic changes in the GSH redox state in the organelle and pave the way for further characterization of the Golgi redox state under various physiological and pathological conditions. |
| format | Article |
| id | doaj-art-82ffac8bbd134697bd9972d8632d9761 |
| institution | OA Journals |
| issn | 2213-2317 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj-art-82ffac8bbd134697bd9972d8632d97612025-08-20T02:07:17ZengElsevierRedox Biology2213-23172025-04-018110356010.1016/j.redox.2025.103560Characterization of the glutathione redox state in the Golgi apparatusCarla Miró-Vinyals0Sarah Emmert1Gina Grammbitter2Alex Jud3Tobias Kockmann4Pablo Rivera-Fuentes5Department of Chemistry, University of Zurich, Zurich, SwitzerlandDepartment of Chemistry, University of Zurich, Zurich, SwitzerlandInstitute of Chemical Sciences and Engineering, École Polytechnique Fédéral de Lausanne, Lausanne, SwitzerlandDepartment of Biochemistry, University of Zurich, Zurich, SwitzerlandFunctional Genomics Center Zurich, ETH Zurich/University of Zurich, Zurich, SwitzerlandDepartment of Chemistry, University of Zurich, Zurich, Switzerland; Corresponding author.Redox homeostasis is crucial for cell function, and, in eukaryotic cells, studying it in a compartmentalized way is essential due to the redox variations between different organelles. The redox state of organelles is largely determined by the redox potential of glutathione, EGSH, and the concentration of its reduced and oxidized species, [GS]. The Golgi apparatus is an essential component of the secretory pathway, yet little is known about the concentration or redox state of GSH in this organelle. Here, we characterized the redox state of GSH in the Golgi apparatus using a combination of microscopy and proteomics methods. Our results prove that the Golgi apparatus is a highly oxidizing organelle with a strikingly low GSH concentration (EGSH = – 157 mV, 1–5 mM). These results fill an important gap in our knowledge of redox homeostasis in subcellular organelles. Moreover, the new Golgi-targeted GSH sensors allow us to observe dynamic changes in the GSH redox state in the organelle and pave the way for further characterization of the Golgi redox state under various physiological and pathological conditions.http://www.sciencedirect.com/science/article/pii/S2213231725000734Golgi apparatusroGFPRedox homeostasisGlutathione |
| spellingShingle | Carla Miró-Vinyals Sarah Emmert Gina Grammbitter Alex Jud Tobias Kockmann Pablo Rivera-Fuentes Characterization of the glutathione redox state in the Golgi apparatus Redox Biology Golgi apparatus roGFP Redox homeostasis Glutathione |
| title | Characterization of the glutathione redox state in the Golgi apparatus |
| title_full | Characterization of the glutathione redox state in the Golgi apparatus |
| title_fullStr | Characterization of the glutathione redox state in the Golgi apparatus |
| title_full_unstemmed | Characterization of the glutathione redox state in the Golgi apparatus |
| title_short | Characterization of the glutathione redox state in the Golgi apparatus |
| title_sort | characterization of the glutathione redox state in the golgi apparatus |
| topic | Golgi apparatus roGFP Redox homeostasis Glutathione |
| url | http://www.sciencedirect.com/science/article/pii/S2213231725000734 |
| work_keys_str_mv | AT carlamirovinyals characterizationoftheglutathioneredoxstateinthegolgiapparatus AT sarahemmert characterizationoftheglutathioneredoxstateinthegolgiapparatus AT ginagrammbitter characterizationoftheglutathioneredoxstateinthegolgiapparatus AT alexjud characterizationoftheglutathioneredoxstateinthegolgiapparatus AT tobiaskockmann characterizationoftheglutathioneredoxstateinthegolgiapparatus AT pabloriverafuentes characterizationoftheglutathioneredoxstateinthegolgiapparatus |