Cadmium treatment effects on the growth and antioxidant system in barley plants under optimal and low temperatures
The cadmium effect (100 μM) on the barley (Hordeum vulgare L.) growth, the content of HvCu/ZnSOD, HvCAT2 and HvPRX07 transcripts and the antioxidant enzymes activity (SOD, CAT and PRX) in roots and leaves of seedlings under optimal (22 °C) and low (4 °C) temperatures were studied. Exposure to cadmiu...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2018-04-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/12759 |
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| author | Natalia KAZNINA Julia BATOVA Natalia REPKINA Galina LAIDINEN Alexandr TITOV |
| author_facet | Natalia KAZNINA Julia BATOVA Natalia REPKINA Galina LAIDINEN Alexandr TITOV |
| author_sort | Natalia KAZNINA |
| collection | DOAJ |
| description | The cadmium effect (100 μM) on the barley (Hordeum vulgare L.) growth, the content of HvCu/ZnSOD, HvCAT2 and HvPRX07 transcripts and the antioxidant enzymes activity (SOD, CAT and PRX) in roots and leaves of seedlings under optimal (22 °C) and low (4 °C) temperatures were studied. Exposure to cadmium at 22 °C did not inhibit the plants’ growth. In this case, the rate of the oxidative processes in the cells remained at the control level. This was achieved by a corresponding increase of the gene transcripts and the antioxidant enzymes activity in roots and leaves. In contrast, exposure to cadmium at 4 °C inhibited the seedlings’ growth despite of the lower metal content in the plants. Moreover the rate of lipid peroxidation in the roots and leaves increased significantly. It is assumed that this effect was connected with the accumulation of excess amounts of hydrogen peroxide due to a misbalance between its generation and neutralization. This assumption is confirmed by the obtained data, according to which the level of HvCu/ZnSOD expression and the total activity of SOD increased significantly under exposure to cadmium at 4 °C, although HvCAT2 and HvPRX07 transcripts and CAT and PXR activity did not rise. |
| format | Article |
| id | doaj-art-82f6cd69456948bfa8370b76edac7b52 |
| institution | OA Journals |
| issn | 1854-1941 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-82f6cd69456948bfa8370b76edac7b522025-08-20T02:05:13ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412018-04-01111116917610.14720/aas.2018.111.1.1619151Cadmium treatment effects on the growth and antioxidant system in barley plants under optimal and low temperaturesNatalia KAZNINA0Julia BATOVA1Natalia REPKINA2Galina LAIDINEN3Alexandr TITOV4Institute of Biology, Karelian Research Centre, Russian Academy of Sciences, 11 Pushkinskaya St., 185910 Petrozavodsk, RussiaInstitute of Biology, Karelian Research Centre, Russian Academy of Sciences, 11 Pushkinskaya St., 185910 Petrozavodsk, RussiaInstitute of Biology, Karelian Research Centre, Russian Academy of Sciences, 11 Pushkinskaya St., 185910 Petrozavodsk, RussiaInstitute of Biology, Karelian Research Centre, Russian Academy of Sciences, 11 Pushkinskaya St., 185910 Petrozavodsk, RussiaInstitute of Biology, Karelian Research Centre, Russian Academy of Sciences, 11 Pushkinskaya St., 185910 Petrozavodsk, RussiaThe cadmium effect (100 μM) on the barley (Hordeum vulgare L.) growth, the content of HvCu/ZnSOD, HvCAT2 and HvPRX07 transcripts and the antioxidant enzymes activity (SOD, CAT and PRX) in roots and leaves of seedlings under optimal (22 °C) and low (4 °C) temperatures were studied. Exposure to cadmium at 22 °C did not inhibit the plants’ growth. In this case, the rate of the oxidative processes in the cells remained at the control level. This was achieved by a corresponding increase of the gene transcripts and the antioxidant enzymes activity in roots and leaves. In contrast, exposure to cadmium at 4 °C inhibited the seedlings’ growth despite of the lower metal content in the plants. Moreover the rate of lipid peroxidation in the roots and leaves increased significantly. It is assumed that this effect was connected with the accumulation of excess amounts of hydrogen peroxide due to a misbalance between its generation and neutralization. This assumption is confirmed by the obtained data, according to which the level of HvCu/ZnSOD expression and the total activity of SOD increased significantly under exposure to cadmium at 4 °C, although HvCAT2 and HvPRX07 transcripts and CAT and PXR activity did not rise.https://journals.uni-lj.si/aas/article/view/12759hordeum vulgare l.cadmiumlow temperaturegrowthantioxidant enzymesgene expression |
| spellingShingle | Natalia KAZNINA Julia BATOVA Natalia REPKINA Galina LAIDINEN Alexandr TITOV Cadmium treatment effects on the growth and antioxidant system in barley plants under optimal and low temperatures Acta Agriculturae Slovenica hordeum vulgare l. cadmium low temperature growth antioxidant enzymes gene expression |
| title | Cadmium treatment effects on the growth and antioxidant system in barley plants under optimal and low temperatures |
| title_full | Cadmium treatment effects on the growth and antioxidant system in barley plants under optimal and low temperatures |
| title_fullStr | Cadmium treatment effects on the growth and antioxidant system in barley plants under optimal and low temperatures |
| title_full_unstemmed | Cadmium treatment effects on the growth and antioxidant system in barley plants under optimal and low temperatures |
| title_short | Cadmium treatment effects on the growth and antioxidant system in barley plants under optimal and low temperatures |
| title_sort | cadmium treatment effects on the growth and antioxidant system in barley plants under optimal and low temperatures |
| topic | hordeum vulgare l. cadmium low temperature growth antioxidant enzymes gene expression |
| url | https://journals.uni-lj.si/aas/article/view/12759 |
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