Corticosteroids modulate biofilm formation and virulence of Pseudomonas aeruginosa
Corticosteroids are anti-inflammatory drugs commonly administered to patients with chronic obstructive pulmonary disease (COPD), cystic fibrosis and similar lung pathologies, in which persistent infections with Pseudomonas aeruginosa are frequent. However, their therapeutic value is debatable becaus...
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Elsevier
2025-06-01
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| Series: | Biofilm |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207525000371 |
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| author | Elena Jordana-Lluch María Escobar-Salom Gabriel Torrens Isabel María Barceló Miguel Ángel Estévez Alex González-Alsina Amanda Iglesias Pere Joan Pont-Antona María D. Macià Sebastián Albertí Paul Williams Borja G. Cosío Carlos Juan Antonio Oliver |
| author_facet | Elena Jordana-Lluch María Escobar-Salom Gabriel Torrens Isabel María Barceló Miguel Ángel Estévez Alex González-Alsina Amanda Iglesias Pere Joan Pont-Antona María D. Macià Sebastián Albertí Paul Williams Borja G. Cosío Carlos Juan Antonio Oliver |
| author_sort | Elena Jordana-Lluch |
| collection | DOAJ |
| description | Corticosteroids are anti-inflammatory drugs commonly administered to patients with chronic obstructive pulmonary disease (COPD), cystic fibrosis and similar lung pathologies, in which persistent infections with Pseudomonas aeruginosa are frequent. However, their therapeutic value is debatable because of their adverse impact on host immunity. The aim of this work was to determine the impact of budesonide and fluticasone propionate on P. aeruginosa biology. We found that these corticosteroids attenuated its intrinsic pro-inflammatory properties (reduction of IL-8 release compared to controls ca. 15 % (budesonide) and 50 % (fluticasone propionate)) and cellular invasiveness (25 % and 40 % respectively). Corticosteroids enhanced P. aeruginosa biofilm formation in a time/dose-dependent manner (around 1.6-fold for the highest concentration, with this increase occurring more readily in sputum media)) and stimulated the release of extracellular DNA (2-fold increase), a key component of the biofilm matrix. Regarding the mechanisms involved, our results suggest that corticosteroids diffuse through P. aeruginosa's membrane influencing its fluidity and triggering cell envelope stress signalling pathways, as shown by an initial increase in mucA (σ22 regulon) expression, outer membrane vesicle release and accumulation of cyclic diguanylate (c-di-GMP). Changes in the levels of this intracellular signalling molecule, responsible for the switch from planktonic to biofilm lifestyle, may explain some of the phenotypes observed. In conclusion, our data, first obtained with type strains and proved to be reproducible when using COPD clinical isolates, suggest that corticosteroids could mediate a faster acquisition of the phenotypic characteristics associated with P. aeruginosa long-term adaptation to the chronic lung niche without undergoing mutation. |
| format | Article |
| id | doaj-art-82e2c095eb5d4d9288b3558dc316481d |
| institution | OA Journals |
| issn | 2590-2075 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj-art-82e2c095eb5d4d9288b3558dc316481d2025-08-20T02:05:13ZengElsevierBiofilm2590-20752025-06-01910028910.1016/j.bioflm.2025.100289Corticosteroids modulate biofilm formation and virulence of Pseudomonas aeruginosaElena Jordana-Lluch0María Escobar-Salom1Gabriel Torrens2Isabel María Barceló3Miguel Ángel Estévez4Alex González-Alsina5Amanda Iglesias6Pere Joan Pont-Antona7María D. Macià8Sebastián Albertí9Paul Williams10Borja G. Cosío11Carlos Juan12Antonio Oliver13Health Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Microbiology Department, University Hospital Son Espases, 07010, Palma, Spain; Centro de Investigación Biomédica en Red en Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC), 28029, Madrid, Spain; Corresponding author Health Research Institute of the Balearic Islands (IdISBa), 07010, Palma, SpainHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Microbiology Department, University Hospital Son Espases, 07010, Palma, Spain; Centro de Investigación Biomédica en Red en Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC), 28029, Madrid, SpainHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Microbiology Department, University Hospital Son Espases, 07010, Palma, SpainHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Microbiology Department, University Hospital Son Espases, 07010, Palma, Spain; Centro de Investigación Biomédica en Red en Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC), 28029, Madrid, SpainHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Microbiology Department, University Hospital Son Espases, 07010, Palma, SpainHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Research Institute of Health Sciences (IUNICS), University of the Balearic Islands, 07122, Palma de Mallorca, SpainHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias, Instituto de Salud Carlos III (CIBERES), 28029, Madrid, SpainHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, SpainHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Microbiology Department, University Hospital Son Espases, 07010, Palma, Spain; Centro de Investigación Biomédica en Red en Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC), 28029, Madrid, SpainHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Research Institute of Health Sciences (IUNICS), University of the Balearic Islands, 07122, Palma de Mallorca, SpainBiodiscovery Institute and School of Life Sciences, University Park, University of Nottingham, Nottingham, NG7 2RD, United KingdomHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Centro de Investigación Biomédica en Red de Enfermedades Respiratorias, Instituto de Salud Carlos III (CIBERES), 28029, Madrid, Spain; Department of Respiratory Medicine, University Hospital Son Espases, 07010, Palma, SpainHealth Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Microbiology Department, University Hospital Son Espases, 07010, Palma, Spain; Centro de Investigación Biomédica en Red en Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC), 28029, Madrid, Spain; Corresponding author. Health Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain.Health Research Institute of the Balearic Islands (IdISBa), 07010, Palma, Spain; Microbiology Department, University Hospital Son Espases, 07010, Palma, Spain; Centro de Investigación Biomédica en Red en Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC), 28029, Madrid, SpainCorticosteroids are anti-inflammatory drugs commonly administered to patients with chronic obstructive pulmonary disease (COPD), cystic fibrosis and similar lung pathologies, in which persistent infections with Pseudomonas aeruginosa are frequent. However, their therapeutic value is debatable because of their adverse impact on host immunity. The aim of this work was to determine the impact of budesonide and fluticasone propionate on P. aeruginosa biology. We found that these corticosteroids attenuated its intrinsic pro-inflammatory properties (reduction of IL-8 release compared to controls ca. 15 % (budesonide) and 50 % (fluticasone propionate)) and cellular invasiveness (25 % and 40 % respectively). Corticosteroids enhanced P. aeruginosa biofilm formation in a time/dose-dependent manner (around 1.6-fold for the highest concentration, with this increase occurring more readily in sputum media)) and stimulated the release of extracellular DNA (2-fold increase), a key component of the biofilm matrix. Regarding the mechanisms involved, our results suggest that corticosteroids diffuse through P. aeruginosa's membrane influencing its fluidity and triggering cell envelope stress signalling pathways, as shown by an initial increase in mucA (σ22 regulon) expression, outer membrane vesicle release and accumulation of cyclic diguanylate (c-di-GMP). Changes in the levels of this intracellular signalling molecule, responsible for the switch from planktonic to biofilm lifestyle, may explain some of the phenotypes observed. In conclusion, our data, first obtained with type strains and proved to be reproducible when using COPD clinical isolates, suggest that corticosteroids could mediate a faster acquisition of the phenotypic characteristics associated with P. aeruginosa long-term adaptation to the chronic lung niche without undergoing mutation.http://www.sciencedirect.com/science/article/pii/S2590207525000371Pseudomonas aeruginosaChronic obstructive pulmonary diseaseCystic fibrosisBiofilmCorticosteroidsBudesonide |
| spellingShingle | Elena Jordana-Lluch María Escobar-Salom Gabriel Torrens Isabel María Barceló Miguel Ángel Estévez Alex González-Alsina Amanda Iglesias Pere Joan Pont-Antona María D. Macià Sebastián Albertí Paul Williams Borja G. Cosío Carlos Juan Antonio Oliver Corticosteroids modulate biofilm formation and virulence of Pseudomonas aeruginosa Biofilm Pseudomonas aeruginosa Chronic obstructive pulmonary disease Cystic fibrosis Biofilm Corticosteroids Budesonide |
| title | Corticosteroids modulate biofilm formation and virulence of Pseudomonas aeruginosa |
| title_full | Corticosteroids modulate biofilm formation and virulence of Pseudomonas aeruginosa |
| title_fullStr | Corticosteroids modulate biofilm formation and virulence of Pseudomonas aeruginosa |
| title_full_unstemmed | Corticosteroids modulate biofilm formation and virulence of Pseudomonas aeruginosa |
| title_short | Corticosteroids modulate biofilm formation and virulence of Pseudomonas aeruginosa |
| title_sort | corticosteroids modulate biofilm formation and virulence of pseudomonas aeruginosa |
| topic | Pseudomonas aeruginosa Chronic obstructive pulmonary disease Cystic fibrosis Biofilm Corticosteroids Budesonide |
| url | http://www.sciencedirect.com/science/article/pii/S2590207525000371 |
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