Identification of a novel transcript of mouse Sdha
Abstract Objective This study aimed to identify novel isoforms of mouse succinate dehydrogenase complex flavoprotein subunit A (Sdha) arising from internal exon skipping, analogous to the process observed in human ortholog SDHA. Results We identified a novel isoform, designated Δ3-10, which lacked t...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-025-07149-8 |
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| author | María J. Esteban-Amo Amaia Telleria Dino Gobelli Pablo Serrano-Lorenzo Juan J. Tellería María T. Pérez-García Piotr Kozlowski Miguel Á. de la Fuente María Simarro |
| author_facet | María J. Esteban-Amo Amaia Telleria Dino Gobelli Pablo Serrano-Lorenzo Juan J. Tellería María T. Pérez-García Piotr Kozlowski Miguel Á. de la Fuente María Simarro |
| author_sort | María J. Esteban-Amo |
| collection | DOAJ |
| description | Abstract Objective This study aimed to identify novel isoforms of mouse succinate dehydrogenase complex flavoprotein subunit A (Sdha) arising from internal exon skipping, analogous to the process observed in human ortholog SDHA. Results We identified a novel isoform, designated Δ3-10, which lacked the final 104 nucleotides of exon 3 and all of exons 4 through 10, yet did not alter the reading frame. The Δ3-10 Sdha cDNA was cloned into expression vectors, and overexpression resulted in a protein localized to the mitochondria. However, the endogenous Δ3-10 Sdha protein was not detected with the available antibodies. |
| format | Article |
| id | doaj-art-82dedf8e368c43d2ab88029793f41eb9 |
| institution | DOAJ |
| issn | 1756-0500 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-82dedf8e368c43d2ab88029793f41eb92025-08-20T03:03:57ZengBMCBMC Research Notes1756-05002025-02-011811710.1186/s13104-025-07149-8Identification of a novel transcript of mouse SdhaMaría J. Esteban-Amo0Amaia Telleria1Dino Gobelli2Pablo Serrano-Lorenzo3Juan J. Tellería4María T. Pérez-García5Piotr Kozlowski6Miguel Á. de la Fuente7María Simarro8Department of Cell Biology, Genetics, Histology and Pharmacology, Faculty of Medicine, University of ValladolidDepartment of Cell Biology, Genetics, Histology and Pharmacology, Faculty of Medicine, University of ValladolidDepartment of Cell Biology, Genetics, Histology and Pharmacology, Faculty of Medicine, University of ValladolidHospital 12 de Octubre Research Institute (imas12)Department of Cell Biology, Genetics, Histology and Pharmacology, Faculty of Medicine, University of ValladolidUnit of Excellence Institute of Biomedicine and Molecular Genetics (IBGM), University of Valladolid and Spanish National Research Council (CSIC)Department of Molecular Genetics, Institute of Bioorganic Chemistry, Polish Academy of SciencesDepartment of Cell Biology, Genetics, Histology and Pharmacology, Faculty of Medicine, University of ValladolidDepartment of Cell Biology, Genetics, Histology and Pharmacology, Faculty of Medicine, University of ValladolidAbstract Objective This study aimed to identify novel isoforms of mouse succinate dehydrogenase complex flavoprotein subunit A (Sdha) arising from internal exon skipping, analogous to the process observed in human ortholog SDHA. Results We identified a novel isoform, designated Δ3-10, which lacked the final 104 nucleotides of exon 3 and all of exons 4 through 10, yet did not alter the reading frame. The Δ3-10 Sdha cDNA was cloned into expression vectors, and overexpression resulted in a protein localized to the mitochondria. However, the endogenous Δ3-10 Sdha protein was not detected with the available antibodies.https://doi.org/10.1186/s13104-025-07149-8SdhaIsoformsMitochondriaMouse |
| spellingShingle | María J. Esteban-Amo Amaia Telleria Dino Gobelli Pablo Serrano-Lorenzo Juan J. Tellería María T. Pérez-García Piotr Kozlowski Miguel Á. de la Fuente María Simarro Identification of a novel transcript of mouse Sdha BMC Research Notes Sdha Isoforms Mitochondria Mouse |
| title | Identification of a novel transcript of mouse Sdha |
| title_full | Identification of a novel transcript of mouse Sdha |
| title_fullStr | Identification of a novel transcript of mouse Sdha |
| title_full_unstemmed | Identification of a novel transcript of mouse Sdha |
| title_short | Identification of a novel transcript of mouse Sdha |
| title_sort | identification of a novel transcript of mouse sdha |
| topic | Sdha Isoforms Mitochondria Mouse |
| url | https://doi.org/10.1186/s13104-025-07149-8 |
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