A pilot study on the incorporation of adipose-derived stem cells into bone cements to enhance bone defect regeneration
Advancements in regenerative medicine increasingly focus on developing scaffolds that not only support stem cell proliferation but also guide their differentiation into specific cell types essential for tissue repair. Adipose-derived stem cells (ASCs) are considered a promising option for...
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Academia.edu Journals
2025-06-01
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| Series: | Academia Materials Science |
| Online Access: | https://www.academia.edu/129871058/A_pilot_study_on_the_incorporation_of_adipose_derived_stem_cells_into_bone_cements_to_enhance_bone_defect_regeneration |
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| author | Julia Lucas-Aparicio Jatsue Cabrejos-Azama Jesús Torres Carmen Rueda Mohammad-H. Alkhraisat Luis Blanco-Jerez Enrique López-Cabarcos |
| author_facet | Julia Lucas-Aparicio Jatsue Cabrejos-Azama Jesús Torres Carmen Rueda Mohammad-H. Alkhraisat Luis Blanco-Jerez Enrique López-Cabarcos |
| author_sort | Julia Lucas-Aparicio |
| collection | DOAJ |
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Advancements in regenerative medicine increasingly focus on developing scaffolds that not only support stem cell proliferation but also guide their differentiation into specific cell types essential for tissue repair. Adipose-derived stem cells (ASCs) are considered a promising option for bone regeneration therapies, primarily because they are readily accessible and can be obtained in large quantities with minimally invasive procedures. This study aims to establish a straightforward and effective novel experimental model for incorporating ASCs into silicon calcium phosphate cement (Si-CPC), potentially enhancing the scaffold’s osteoconductive properties and facilitating bone tissue regeneration. The in vivo evaluation of the cement was carried out using a rabbit model, where titanium cylinders were implanted into pre-drilled slits in the parietal cortical bone. One cylinder served as a control and was filled with Si-CPC alone, while the other received Si-CPC combined with adipose-derived stem cells applied across the surface of the granules. Four weeks after the intervention, histology and histomorphometry tests are performed. In the histology test, the Si-CPC with ASCs presented the training of bony bridges between the material granules. We will need a larger sample to evaluate the amount of new bone formed. However, we can conclude that this new method of incorporating stem cells into bone cements could be a promising tool in bone engineering. |
| format | Article |
| id | doaj-art-82dd63c04cb44f1bb6d6a137d86e747c |
| institution | Kabale University |
| issn | 2997-2027 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Academia.edu Journals |
| record_format | Article |
| series | Academia Materials Science |
| spelling | doaj-art-82dd63c04cb44f1bb6d6a137d86e747c2025-08-20T03:30:19ZengAcademia.edu JournalsAcademia Materials Science2997-20272025-06-012210.20935/AcadMatSci7764A pilot study on the incorporation of adipose-derived stem cells into bone cements to enhance bone defect regenerationJulia Lucas-Aparicio0Jatsue Cabrejos-Azama1Jesús Torres2Carmen Rueda3Mohammad-H. Alkhraisat4Luis Blanco-Jerez5Enrique López-Cabarcos6Department of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain.Department of Dental Clinical Specialties, Faculty of Dentistry, Complutense University of Madrid, 28040 Madrid, Spain.Department of Dental Clinical Specialties, Faculty of Dentistry, Complutense University of Madrid, 28040 Madrid, Spain.Department of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain.BTI Biotechnology Institute, Vitoria-Gasteiz 01005, Álava, Spain.Department of Stomatology III, Faculty of Dentistry, Complutense University of Madrid, 28040 Madrid, Spain.Department of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain. Advancements in regenerative medicine increasingly focus on developing scaffolds that not only support stem cell proliferation but also guide their differentiation into specific cell types essential for tissue repair. Adipose-derived stem cells (ASCs) are considered a promising option for bone regeneration therapies, primarily because they are readily accessible and can be obtained in large quantities with minimally invasive procedures. This study aims to establish a straightforward and effective novel experimental model for incorporating ASCs into silicon calcium phosphate cement (Si-CPC), potentially enhancing the scaffold’s osteoconductive properties and facilitating bone tissue regeneration. The in vivo evaluation of the cement was carried out using a rabbit model, where titanium cylinders were implanted into pre-drilled slits in the parietal cortical bone. One cylinder served as a control and was filled with Si-CPC alone, while the other received Si-CPC combined with adipose-derived stem cells applied across the surface of the granules. Four weeks after the intervention, histology and histomorphometry tests are performed. In the histology test, the Si-CPC with ASCs presented the training of bony bridges between the material granules. We will need a larger sample to evaluate the amount of new bone formed. However, we can conclude that this new method of incorporating stem cells into bone cements could be a promising tool in bone engineering.https://www.academia.edu/129871058/A_pilot_study_on_the_incorporation_of_adipose_derived_stem_cells_into_bone_cements_to_enhance_bone_defect_regeneration |
| spellingShingle | Julia Lucas-Aparicio Jatsue Cabrejos-Azama Jesús Torres Carmen Rueda Mohammad-H. Alkhraisat Luis Blanco-Jerez Enrique López-Cabarcos A pilot study on the incorporation of adipose-derived stem cells into bone cements to enhance bone defect regeneration Academia Materials Science |
| title | A pilot study on the incorporation of adipose-derived stem cells into bone cements to enhance bone defect regeneration |
| title_full | A pilot study on the incorporation of adipose-derived stem cells into bone cements to enhance bone defect regeneration |
| title_fullStr | A pilot study on the incorporation of adipose-derived stem cells into bone cements to enhance bone defect regeneration |
| title_full_unstemmed | A pilot study on the incorporation of adipose-derived stem cells into bone cements to enhance bone defect regeneration |
| title_short | A pilot study on the incorporation of adipose-derived stem cells into bone cements to enhance bone defect regeneration |
| title_sort | pilot study on the incorporation of adipose derived stem cells into bone cements to enhance bone defect regeneration |
| url | https://www.academia.edu/129871058/A_pilot_study_on_the_incorporation_of_adipose_derived_stem_cells_into_bone_cements_to_enhance_bone_defect_regeneration |
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