In-vitro acaricidal activity of peganum harmala and glaucium flavum alkaloid against rhipicephalus sp. of dog

<b>Aim: </b>The major problem of drug intended for tick control is resistance developed to application doses on the hosts. The acaricidal effects of alkaloids from Glaucium flavum and Peganum harmala were evaluated in vitro on adult female of Rhipicephalus sp. collected in naturally infe...

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Main Authors: Rosa Kebbi, Omar Besseboua, Mohamed Nait-Mouloud, Abdelhanine Ayad
Format: Article
Language:English
Published: Selcuk University Press 2020-03-01
Series:Eurasian Journal of Veterinary Sciences
Subjects:
Online Access:http://eurasianjvetsci.org/pdf.php3?id=1275
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author Rosa Kebbi
Omar Besseboua
Mohamed Nait-Mouloud
Abdelhanine Ayad
author_facet Rosa Kebbi
Omar Besseboua
Mohamed Nait-Mouloud
Abdelhanine Ayad
author_sort Rosa Kebbi
collection DOAJ
description <b>Aim: </b>The major problem of drug intended for tick control is resistance developed to application doses on the hosts. The acaricidal effects of alkaloids from Glaucium flavum and Peganum harmala were evaluated in vitro on adult female of Rhipicephalus sp. collected in naturally infected dogs.<p> <b>Materials and Methods: </b>The acaricide activities of the both extracts (3.12, 6.2, 12.5, 25 and 50 mg/ml) were evaluated using the adult immersion test, reproductive index and inhibition oviposition. DMSO (1%) and SEB (1?l/ml) were used as a negative and positive control.<p> <b>Results:</b> The percent mortality caused by the methanolic extracts of both plants varied from 41.66 to 75 %, when tested at concentrations ranging from 3.12 to 50 mg/ml, and significantly different compared to control (p<0.05). Visually hatching of the eggs was completely blocked only by G. flavum with 50 mg/ml; however, other extracts of both plants were partially able to block the hatching. The oviposition and reproductive rate of both plants were concentration dependent in both plants and significantly different (p<0.05) compared to negative control. Also, the extracts of P. harmala seem to be more efficient against Rhipicephalus sp. adults at different concentrations than the extracts G. flavum. The alkaloid extracts of both plants high effects were observed with 6.25 and 50 mg/ml for G. flavum and with 25mg/ml and 50mg/ml for P. harmala.<p> <b>Conclusion: </b>The total alkaloids extracted from the G. flavum root and P. harmala seed have good acaricidal activities in vitro reducing potentially the egg laying of Rhipicephalus sp. females.
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publishDate 2020-03-01
publisher Selcuk University Press
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series Eurasian Journal of Veterinary Sciences
spelling doaj-art-82db02015c3a4819be3e96835080bb5c2025-02-03T11:25:38ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532020-03-0136128351275In-vitro acaricidal activity of peganum harmala and glaucium flavum alkaloid against rhipicephalus sp. of dogRosa Kebbi0Omar Besseboua1Mohamed Nait-Mouloud2Abdelhanine Ayad3Department of Environment Biological Sciences, Faculty of Nature and Life Sciences, University of Bejaia, Route de Targa Ouzemmour, Bejaia, AlgeriaDepartment of Agronomic and Biotechnological Sciences, Faculty of Nature and Life Sciences, University Hassiba Benbouali, Chlef, AlgeriaDepartment of Environment Biological Sciences, Faculty of Nature and Life Sciences, University of Bejaia, Route de Targa Ouzemmour, Bejaia, AlgeriaDepartment of Environment Biological Sciences, Faculty of Nature and Life Sciences, University of Bejaia, Route de Targa Ouzemmour, Bejaia, Algeria<b>Aim: </b>The major problem of drug intended for tick control is resistance developed to application doses on the hosts. The acaricidal effects of alkaloids from Glaucium flavum and Peganum harmala were evaluated in vitro on adult female of Rhipicephalus sp. collected in naturally infected dogs.<p> <b>Materials and Methods: </b>The acaricide activities of the both extracts (3.12, 6.2, 12.5, 25 and 50 mg/ml) were evaluated using the adult immersion test, reproductive index and inhibition oviposition. DMSO (1%) and SEB (1?l/ml) were used as a negative and positive control.<p> <b>Results:</b> The percent mortality caused by the methanolic extracts of both plants varied from 41.66 to 75 %, when tested at concentrations ranging from 3.12 to 50 mg/ml, and significantly different compared to control (p<0.05). Visually hatching of the eggs was completely blocked only by G. flavum with 50 mg/ml; however, other extracts of both plants were partially able to block the hatching. The oviposition and reproductive rate of both plants were concentration dependent in both plants and significantly different (p<0.05) compared to negative control. Also, the extracts of P. harmala seem to be more efficient against Rhipicephalus sp. adults at different concentrations than the extracts G. flavum. The alkaloid extracts of both plants high effects were observed with 6.25 and 50 mg/ml for G. flavum and with 25mg/ml and 50mg/ml for P. harmala.<p> <b>Conclusion: </b>The total alkaloids extracted from the G. flavum root and P. harmala seed have good acaricidal activities in vitro reducing potentially the egg laying of Rhipicephalus sp. females.http://eurasianjvetsci.org/pdf.php3?id=1275alkaloidacaricidein vitrorhipicephalus sp.dogs
spellingShingle Rosa Kebbi
Omar Besseboua
Mohamed Nait-Mouloud
Abdelhanine Ayad
In-vitro acaricidal activity of peganum harmala and glaucium flavum alkaloid against rhipicephalus sp. of dog
Eurasian Journal of Veterinary Sciences
alkaloid
acaricide
in vitro
rhipicephalus sp.
dogs
title In-vitro acaricidal activity of peganum harmala and glaucium flavum alkaloid against rhipicephalus sp. of dog
title_full In-vitro acaricidal activity of peganum harmala and glaucium flavum alkaloid against rhipicephalus sp. of dog
title_fullStr In-vitro acaricidal activity of peganum harmala and glaucium flavum alkaloid against rhipicephalus sp. of dog
title_full_unstemmed In-vitro acaricidal activity of peganum harmala and glaucium flavum alkaloid against rhipicephalus sp. of dog
title_short In-vitro acaricidal activity of peganum harmala and glaucium flavum alkaloid against rhipicephalus sp. of dog
title_sort in vitro acaricidal activity of peganum harmala and glaucium flavum alkaloid against rhipicephalus sp of dog
topic alkaloid
acaricide
in vitro
rhipicephalus sp.
dogs
url http://eurasianjvetsci.org/pdf.php3?id=1275
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AT mohamednaitmouloud invitroacaricidalactivityofpeganumharmalaandglauciumflavumalkaloidagainstrhipicephalusspofdog
AT abdelhanineayad invitroacaricidalactivityofpeganumharmalaandglauciumflavumalkaloidagainstrhipicephalusspofdog