Differential Ca2+ handling by isolated synaptic and non-synaptic mitochondria: roles of Ca2+ buffering and efflux

Mitochondria regulate intracellular calcium ion (Ca2+) signaling by a fine-tuned process of mitochondrial matrix (m) Ca2+ influx, mCa2+ buffering (sequestration) and mCa2+ release (Ca2+ efflux). This process is critically important in the neurosynaptic terminal, where there is a simultaneous high de...

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Main Authors: Jyotsna Mishra, Kyle Bevers, Keguo Li, Armaan Zare, James S. Heisner, Ailing Tong, Wai-Meng Kwok, David F. Stowe, Amadou K. S. Camara
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Synaptic Neuroscience
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Online Access:https://www.frontiersin.org/articles/10.3389/fnsyn.2025.1562065/full
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author Jyotsna Mishra
Kyle Bevers
Keguo Li
Armaan Zare
James S. Heisner
Ailing Tong
Wai-Meng Kwok
Wai-Meng Kwok
Wai-Meng Kwok
Wai-Meng Kwok
David F. Stowe
David F. Stowe
David F. Stowe
David F. Stowe
Amadou K. S. Camara
Amadou K. S. Camara
Amadou K. S. Camara
Amadou K. S. Camara
author_facet Jyotsna Mishra
Kyle Bevers
Keguo Li
Armaan Zare
James S. Heisner
Ailing Tong
Wai-Meng Kwok
Wai-Meng Kwok
Wai-Meng Kwok
Wai-Meng Kwok
David F. Stowe
David F. Stowe
David F. Stowe
David F. Stowe
Amadou K. S. Camara
Amadou K. S. Camara
Amadou K. S. Camara
Amadou K. S. Camara
author_sort Jyotsna Mishra
collection DOAJ
description Mitochondria regulate intracellular calcium ion (Ca2+) signaling by a fine-tuned process of mitochondrial matrix (m) Ca2+ influx, mCa2+ buffering (sequestration) and mCa2+ release (Ca2+ efflux). This process is critically important in the neurosynaptic terminal, where there is a simultaneous high demand for ATP utilization, cytosolic (c) Ca2+ regulation, and maintenance of ionic gradients across the cell membrane. Brain synaptic and non-synaptic mitochondria display marked differences in Ca2+ retention capacity. We hypothesized that mitochondrial Ca2+ handling in these two mitochondrial populations is determined by the net effects of Ca2+ uptake, buffering or efflux with increasing CaCl2 boluses. We found first that synaptic mitochondria have a more coupled respiration than non-synaptic mitochondria; this may correlate with the higher local energy demand in synapses to support neurotransmission. When both mitochondrial fractions were exposed to increasing mCa2+ loads we observed decreased mCa2+ sequestration in synaptic mitochondria as assessed by a significant increase in the steady-state free extra matrix Ca2+ (ss[Ca2+]e) compared to non-synaptic mitochondria. Since, non-synaptic mitochondria displayed a significantly reduced ss[Ca2+]e, this suggested a larger mCa2+ buffering capacity to maintain [Ca2+]m with increasing mCa2+ loads. There were no differences in the magnitude of the transient depolarizations and repolarizations of the membrane potential (ΔΨm) and both fractions exhibited similar gradual depolarization of the baseline ΔΨm during additional CaCl2 boluses. Adding the mitochondrial Na+/Ca2+ exchanger (mNCE) inhibitor CGP37157 to the mitochondrial suspensions unmasked the mCa2+ sequestration and concomitantly lowered ss[Ca2+]e in synaptic vs. non-synaptic mitochondria. Adding complex V inhibitor oligomycin plus ADP (OMN + ADP) bolstered the matrix Ca2+ buffering capacity in synaptic mitochondria, as did Cyclosporin A (CsA), in non-synaptic. Our results display distinct differences in regulation of the free [Ca2+]m to prevent collapse of ΔΨm during mCa2+ overload in the two populations of mitochondria. Synaptic mitochondria appear to rely mainly on mCa2+ efflux via mNCE, while non-synaptic mitochondria rely mainly on Pi-dependent mCa2+ sequestration. The functional implications of differential mCa2+ handling at neuronal synapses may be adaptations to cope with the higher metabolic activity and larger mCa2+ transients at synaptosomes, reflecting a distinct role they play in brain function.
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series Frontiers in Synaptic Neuroscience
spelling doaj-art-82cf012ba629403e9b2b2bc5b03ef8622025-08-20T01:57:01ZengFrontiers Media S.A.Frontiers in Synaptic Neuroscience1663-35632025-05-011710.3389/fnsyn.2025.15620651562065Differential Ca2+ handling by isolated synaptic and non-synaptic mitochondria: roles of Ca2+ buffering and effluxJyotsna Mishra0Kyle Bevers1Keguo Li2Armaan Zare3James S. Heisner4Ailing Tong5Wai-Meng Kwok6Wai-Meng Kwok7Wai-Meng Kwok8Wai-Meng Kwok9David F. Stowe10David F. Stowe11David F. Stowe12David F. Stowe13Amadou K. S. Camara14Amadou K. S. Camara15Amadou K. S. Camara16Amadou K. S. Camara17Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, United StatesCardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United StatesCancer Center, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United StatesCardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Biomedical Engineering, Medical College of Wisconsin and Marquette University, Milwaukee, WI, United StatesDepartment of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, United StatesCardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United StatesCancer Center, Medical College of Wisconsin, Milwaukee, WI, United StatesDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, United StatesMitochondria regulate intracellular calcium ion (Ca2+) signaling by a fine-tuned process of mitochondrial matrix (m) Ca2+ influx, mCa2+ buffering (sequestration) and mCa2+ release (Ca2+ efflux). This process is critically important in the neurosynaptic terminal, where there is a simultaneous high demand for ATP utilization, cytosolic (c) Ca2+ regulation, and maintenance of ionic gradients across the cell membrane. Brain synaptic and non-synaptic mitochondria display marked differences in Ca2+ retention capacity. We hypothesized that mitochondrial Ca2+ handling in these two mitochondrial populations is determined by the net effects of Ca2+ uptake, buffering or efflux with increasing CaCl2 boluses. We found first that synaptic mitochondria have a more coupled respiration than non-synaptic mitochondria; this may correlate with the higher local energy demand in synapses to support neurotransmission. When both mitochondrial fractions were exposed to increasing mCa2+ loads we observed decreased mCa2+ sequestration in synaptic mitochondria as assessed by a significant increase in the steady-state free extra matrix Ca2+ (ss[Ca2+]e) compared to non-synaptic mitochondria. Since, non-synaptic mitochondria displayed a significantly reduced ss[Ca2+]e, this suggested a larger mCa2+ buffering capacity to maintain [Ca2+]m with increasing mCa2+ loads. There were no differences in the magnitude of the transient depolarizations and repolarizations of the membrane potential (ΔΨm) and both fractions exhibited similar gradual depolarization of the baseline ΔΨm during additional CaCl2 boluses. Adding the mitochondrial Na+/Ca2+ exchanger (mNCE) inhibitor CGP37157 to the mitochondrial suspensions unmasked the mCa2+ sequestration and concomitantly lowered ss[Ca2+]e in synaptic vs. non-synaptic mitochondria. Adding complex V inhibitor oligomycin plus ADP (OMN + ADP) bolstered the matrix Ca2+ buffering capacity in synaptic mitochondria, as did Cyclosporin A (CsA), in non-synaptic. Our results display distinct differences in regulation of the free [Ca2+]m to prevent collapse of ΔΨm during mCa2+ overload in the two populations of mitochondria. Synaptic mitochondria appear to rely mainly on mCa2+ efflux via mNCE, while non-synaptic mitochondria rely mainly on Pi-dependent mCa2+ sequestration. The functional implications of differential mCa2+ handling at neuronal synapses may be adaptations to cope with the higher metabolic activity and larger mCa2+ transients at synaptosomes, reflecting a distinct role they play in brain function.https://www.frontiersin.org/articles/10.3389/fnsyn.2025.1562065/fullsynaptic mitochondrianon-synaptic mitochondriaCa2+ bufferingCa2+ effluxbioenergetics
spellingShingle Jyotsna Mishra
Kyle Bevers
Keguo Li
Armaan Zare
James S. Heisner
Ailing Tong
Wai-Meng Kwok
Wai-Meng Kwok
Wai-Meng Kwok
Wai-Meng Kwok
David F. Stowe
David F. Stowe
David F. Stowe
David F. Stowe
Amadou K. S. Camara
Amadou K. S. Camara
Amadou K. S. Camara
Amadou K. S. Camara
Differential Ca2+ handling by isolated synaptic and non-synaptic mitochondria: roles of Ca2+ buffering and efflux
Frontiers in Synaptic Neuroscience
synaptic mitochondria
non-synaptic mitochondria
Ca2+ buffering
Ca2+ efflux
bioenergetics
title Differential Ca2+ handling by isolated synaptic and non-synaptic mitochondria: roles of Ca2+ buffering and efflux
title_full Differential Ca2+ handling by isolated synaptic and non-synaptic mitochondria: roles of Ca2+ buffering and efflux
title_fullStr Differential Ca2+ handling by isolated synaptic and non-synaptic mitochondria: roles of Ca2+ buffering and efflux
title_full_unstemmed Differential Ca2+ handling by isolated synaptic and non-synaptic mitochondria: roles of Ca2+ buffering and efflux
title_short Differential Ca2+ handling by isolated synaptic and non-synaptic mitochondria: roles of Ca2+ buffering and efflux
title_sort differential ca2 handling by isolated synaptic and non synaptic mitochondria roles of ca2 buffering and efflux
topic synaptic mitochondria
non-synaptic mitochondria
Ca2+ buffering
Ca2+ efflux
bioenergetics
url https://www.frontiersin.org/articles/10.3389/fnsyn.2025.1562065/full
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