Pattern of sensitization to yellow jacket venom and expression of recombinant antigen 5 (Ves v 5) from yellow jacket venom
Abstract Background Hymenoptera venom allergy is a significant allergic reaction that affects a substantial proportion of adults. Accurate diagnosis of this allergy using venom extracts is challenging due to molecular cross-reactivity. Pure recombinant allergens offer a promising solution to identif...
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2025-03-01
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| Series: | BMC Immunology |
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| Online Access: | https://doi.org/10.1186/s12865-025-00689-5 |
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| author | Sara Mahmoudzadeh Hadis Rezapour Sajjad Chamani Hossein Safarpour Mohammad Fereidouni |
| author_facet | Sara Mahmoudzadeh Hadis Rezapour Sajjad Chamani Hossein Safarpour Mohammad Fereidouni |
| author_sort | Sara Mahmoudzadeh |
| collection | DOAJ |
| description | Abstract Background Hymenoptera venom allergy is a significant allergic reaction that affects a substantial proportion of adults. Accurate diagnosis of this allergy using venom extracts is challenging due to molecular cross-reactivity. Pure recombinant allergens offer a promising solution to identify the specific venom responsible for allergic reactions. This study aimed to produce recombinant phospholipase A5 (Ves v 5) from yellow jacket venom and evaluate the pattern of bee venom sensitization in a group of sensitive patients. Methods and results A total of seven individuals, including four sensitive and three non-sensitive participants, were recruited for this study. Blood samples were collected, and serum was isolated to assess susceptibility to bee venom and recombinant allergens. Expression of Ves v 5 in Escherichia coli resulted in the production of soluble proteins, which were subsequently purified through affinity chromatography. The functionality of the recombinant allergens was evaluated through enzymatic and biophysical analyses, such as dot blot and SDS‒PAGE tests. The diagnostic relevance of Ves v 5 was further investigated using ELISA-based analyses of sera from yellow jacket venom-sensitized patients. Successful production of soluble Ves v 5 in Escherichia coli was achieved. The recombinant Ves v 5 exhibited distinct biochemical and functional characteristics. Evaluation of IgE reactivity in sera from patients underscored the importance of Ves v 5 in hymenoptera venom allergy. Conclusions Our findings suggest that recombinant allergens can serve as an alternative to natural extracts for diagnostic purposes. Furthermore, allergen-specific immunotherapy holds the potential to enhance efficiency and specificity in the treatment of hymenoptera venom allergy. |
| format | Article |
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| institution | DOAJ |
| issn | 1471-2172 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
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| spelling | doaj-art-82b97355867341ac8a4a8f03bab4fc702025-08-20T02:59:19ZengBMCBMC Immunology1471-21722025-03-012611610.1186/s12865-025-00689-5Pattern of sensitization to yellow jacket venom and expression of recombinant antigen 5 (Ves v 5) from yellow jacket venomSara Mahmoudzadeh0Hadis Rezapour1Sajjad Chamani2Hossein Safarpour3Mohammad Fereidouni4Department of Immunology, Faculty of Medicine, Birjand University of Medical SciencesDepartment of Immunology, Faculty of Medicine, Birjand University of Medical SciencesCenter for Immunology and Infection Control, Faculty of Health, School of Biomedical Sciences, Queensland University of Technology (QUT)Department of Immunology, Faculty of Medicine, Birjand University of Medical SciencesDepartment of Immunology, Faculty of Medicine, Birjand University of Medical SciencesAbstract Background Hymenoptera venom allergy is a significant allergic reaction that affects a substantial proportion of adults. Accurate diagnosis of this allergy using venom extracts is challenging due to molecular cross-reactivity. Pure recombinant allergens offer a promising solution to identify the specific venom responsible for allergic reactions. This study aimed to produce recombinant phospholipase A5 (Ves v 5) from yellow jacket venom and evaluate the pattern of bee venom sensitization in a group of sensitive patients. Methods and results A total of seven individuals, including four sensitive and three non-sensitive participants, were recruited for this study. Blood samples were collected, and serum was isolated to assess susceptibility to bee venom and recombinant allergens. Expression of Ves v 5 in Escherichia coli resulted in the production of soluble proteins, which were subsequently purified through affinity chromatography. The functionality of the recombinant allergens was evaluated through enzymatic and biophysical analyses, such as dot blot and SDS‒PAGE tests. The diagnostic relevance of Ves v 5 was further investigated using ELISA-based analyses of sera from yellow jacket venom-sensitized patients. Successful production of soluble Ves v 5 in Escherichia coli was achieved. The recombinant Ves v 5 exhibited distinct biochemical and functional characteristics. Evaluation of IgE reactivity in sera from patients underscored the importance of Ves v 5 in hymenoptera venom allergy. Conclusions Our findings suggest that recombinant allergens can serve as an alternative to natural extracts for diagnostic purposes. Furthermore, allergen-specific immunotherapy holds the potential to enhance efficiency and specificity in the treatment of hymenoptera venom allergy.https://doi.org/10.1186/s12865-025-00689-5AllergyRecombinant proteinsWasp venomsAntigen 5Vespula venom |
| spellingShingle | Sara Mahmoudzadeh Hadis Rezapour Sajjad Chamani Hossein Safarpour Mohammad Fereidouni Pattern of sensitization to yellow jacket venom and expression of recombinant antigen 5 (Ves v 5) from yellow jacket venom BMC Immunology Allergy Recombinant proteins Wasp venoms Antigen 5 Vespula venom |
| title | Pattern of sensitization to yellow jacket venom and expression of recombinant antigen 5 (Ves v 5) from yellow jacket venom |
| title_full | Pattern of sensitization to yellow jacket venom and expression of recombinant antigen 5 (Ves v 5) from yellow jacket venom |
| title_fullStr | Pattern of sensitization to yellow jacket venom and expression of recombinant antigen 5 (Ves v 5) from yellow jacket venom |
| title_full_unstemmed | Pattern of sensitization to yellow jacket venom and expression of recombinant antigen 5 (Ves v 5) from yellow jacket venom |
| title_short | Pattern of sensitization to yellow jacket venom and expression of recombinant antigen 5 (Ves v 5) from yellow jacket venom |
| title_sort | pattern of sensitization to yellow jacket venom and expression of recombinant antigen 5 ves v 5 from yellow jacket venom |
| topic | Allergy Recombinant proteins Wasp venoms Antigen 5 Vespula venom |
| url | https://doi.org/10.1186/s12865-025-00689-5 |
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