Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests
Malaria is the second most prevalent disease in Pakistan resulting in ~30,000 annual deaths. In endemic countries like Pakistan precise and timely diagnosis of malaria is imperative to overcome the associated risks of fatal outcomes. Malarial parasite was screened in 128 malaria suspected patients a...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/9286392 |
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| author | Samina Naz Mukry Madiha Saud Gul Sufaida Kashif Shaikh Arshi Naz Tahir Sultan Shamsi |
| author_facet | Samina Naz Mukry Madiha Saud Gul Sufaida Kashif Shaikh Arshi Naz Tahir Sultan Shamsi |
| author_sort | Samina Naz Mukry |
| collection | DOAJ |
| description | Malaria is the second most prevalent disease in Pakistan resulting in ~30,000 annual deaths. In endemic countries like Pakistan precise and timely diagnosis of malaria is imperative to overcome the associated risks of fatal outcomes. Malarial parasite was screened in 128 malaria suspected patients and 150 healthy controls, by species-specific PCR, microscopy of blood smears, hemoanalyzer Sysmex XE-2100, and rapid test devices (First Response Malaria® and ICT Malaria Combo®). The microscopy detected MP in 126 samples (parasite load/µl 386–53712/µl); 71.094% were infected with Plasmodium vivax and 14.844% with P. falciparum while 14.062% had mixed P. vivax and P. falciparum infection. The mean parasite load for P. vivax and P. falciparum was 14496/µl and 24410/µl, respectively. The abnormal scattergrams of DIFF, WBC/ Baso, IMI channel, and RET-EXT on Sysmex XE-2100 supported 99.2% parasite detection, whereas only 93% of confirmed malaria cases were detected by both rapid tests. About 127 samples were positive by PCR. Since Sysmex XE-2100 automatically detected the presence of malarial parasite with high sensitivity, it can be a good option for presumptive diagnosis in endemic areas. Microscopy remains the gold standard to confirm MP in suspected patients. Rapid diagnostic tests have acceptable sensitivity and specificity. |
| format | Article |
| id | doaj-art-82b73c14b39a48dba20fbed7efd45281 |
| institution | OA Journals |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-82b73c14b39a48dba20fbed7efd452812025-08-20T02:08:46ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932017-01-01201710.1155/2017/92863929286392Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic TestsSamina Naz Mukry0Madiha Saud1Gul Sufaida2Kashif Shaikh3Arshi Naz4Tahir Sultan Shamsi5Division of Immunology & Applied Microbiology, Department of Post Graduate Studies & Research, National Institute of Blood Diseases & Bone Marrow Transplantation, ST 2/A Block 17, Gulshan-e-Iqbal KDA Scheme 24, Karachi, PakistanNational Institute of Blood Diseases & Bone Marrow Transplantation, ST 2/A Block 17, Gulshan-e-Iqbal KDA Scheme 24, Karachi, PakistanNational Institute of Blood Diseases & Bone Marrow Transplantation, ST 2/A Block 17, Gulshan-e-Iqbal KDA Scheme 24, Karachi, PakistanNational Institute of Blood Diseases & Bone Marrow Transplantation, ST 2/A Block 17, Gulshan-e-Iqbal KDA Scheme 24, Karachi, PakistanNational Institute of Blood Diseases & Bone Marrow Transplantation, ST 2/A Block 17, Gulshan-e-Iqbal KDA Scheme 24, Karachi, PakistanNational Institute of Blood Diseases & Bone Marrow Transplantation, ST 2/A Block 17, Gulshan-e-Iqbal KDA Scheme 24, Karachi, PakistanMalaria is the second most prevalent disease in Pakistan resulting in ~30,000 annual deaths. In endemic countries like Pakistan precise and timely diagnosis of malaria is imperative to overcome the associated risks of fatal outcomes. Malarial parasite was screened in 128 malaria suspected patients and 150 healthy controls, by species-specific PCR, microscopy of blood smears, hemoanalyzer Sysmex XE-2100, and rapid test devices (First Response Malaria® and ICT Malaria Combo®). The microscopy detected MP in 126 samples (parasite load/µl 386–53712/µl); 71.094% were infected with Plasmodium vivax and 14.844% with P. falciparum while 14.062% had mixed P. vivax and P. falciparum infection. The mean parasite load for P. vivax and P. falciparum was 14496/µl and 24410/µl, respectively. The abnormal scattergrams of DIFF, WBC/ Baso, IMI channel, and RET-EXT on Sysmex XE-2100 supported 99.2% parasite detection, whereas only 93% of confirmed malaria cases were detected by both rapid tests. About 127 samples were positive by PCR. Since Sysmex XE-2100 automatically detected the presence of malarial parasite with high sensitivity, it can be a good option for presumptive diagnosis in endemic areas. Microscopy remains the gold standard to confirm MP in suspected patients. Rapid diagnostic tests have acceptable sensitivity and specificity.http://dx.doi.org/10.1155/2017/9286392 |
| spellingShingle | Samina Naz Mukry Madiha Saud Gul Sufaida Kashif Shaikh Arshi Naz Tahir Sultan Shamsi Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests |
| title_full | Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests |
| title_fullStr | Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests |
| title_full_unstemmed | Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests |
| title_short | Laboratory Diagnosis of Malaria: Comparison of Manual and Automated Diagnostic Tests |
| title_sort | laboratory diagnosis of malaria comparison of manual and automated diagnostic tests |
| url | http://dx.doi.org/10.1155/2017/9286392 |
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