In Vitro Inhibition of <i>Rhizoctonia oryzae</i>-<i>sativae</i> Using Bacterial Strains as a Sustainable Alternative for Controlling Sheath Blight in Rice
The control of ‘sheath blight’ in rice (<i>Oryza sativa</i> L.), which is caused by the fungus <i>Rhizoctonia oryzae</i>-<i>sativae</i>, has become problematic due to the excessive application of fungicides and their consequent harmful effects. Hence, the search f...
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2024-10-01
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| author | Liz Cheril Quiñones-Pezo Winston Franz Ríos-Ruiz Danny Fran Pompa-Vásquez Franz Rios-Reategui Angel David Hernández-Amasifuen Mike Anderson Corazón-Guivin |
| author_facet | Liz Cheril Quiñones-Pezo Winston Franz Ríos-Ruiz Danny Fran Pompa-Vásquez Franz Rios-Reategui Angel David Hernández-Amasifuen Mike Anderson Corazón-Guivin |
| author_sort | Liz Cheril Quiñones-Pezo |
| collection | DOAJ |
| description | The control of ‘sheath blight’ in rice (<i>Oryza sativa</i> L.), which is caused by the fungus <i>Rhizoctonia oryzae</i>-<i>sativae</i>, has become problematic due to the excessive application of fungicides and their consequent harmful effects. Hence, the search for less contaminating alternatives to conventional chemical products is necessary. This study evaluated the in vitro inhibitory activity of four bacterial strains on the growth of <i>R. oryzae-sativae</i> in both tryptone soy broth (TSB) and mineral medium (MM). The results demonstrated that all evaluated strains (<i>Bacillus tequilensis</i> SMNCT17-02, <i>Priestia aryabhattai</i> SMNCH17-07, <i>Burkholderia vietnamiensis</i> TUR04-01, and <i>Burkholderia vietnamiensis</i> TUR04-03) inhibited the growth of <i>R. oryzae-sativae</i>. Specifically, the activity of <i>B. tequilensis</i> SMNCT17-02 resulted in the smallest area of <i>R. oryzae-sativae</i> growth in both TSB medium (8.54 cm<sup>2</sup>) and MM (5.53 cm<sup>2</sup>), suggesting a notable antifungal effect. When evaluating the action of supernatants generated by the growth of the four bacterial strains in TSB and MM culture media, significant inhibition of <i>R. oryzae-sativae</i> growth was only observed for supernatants produced in MM. This inhibition was attributed to the presence of soluble secondary metabolites. These results offer new perspectives in biotechnology, suggesting the possibility of developing effective products based on easily extractable soluble secondary metabolites, thus promoting sustainable agriculture. |
| format | Article |
| id | doaj-art-82b5c1557e0146c0b7f2b0384ca8dd12 |
| institution | DOAJ |
| issn | 2037-0164 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | International Journal of Plant Biology |
| spelling | doaj-art-82b5c1557e0146c0b7f2b0384ca8dd122025-08-20T02:43:31ZengMDPI AGInternational Journal of Plant Biology2037-01642024-10-01154988100010.3390/ijpb15040070In Vitro Inhibition of <i>Rhizoctonia oryzae</i>-<i>sativae</i> Using Bacterial Strains as a Sustainable Alternative for Controlling Sheath Blight in RiceLiz Cheril Quiñones-Pezo0Winston Franz Ríos-Ruiz1Danny Fran Pompa-Vásquez2Franz Rios-Reategui3Angel David Hernández-Amasifuen4Mike Anderson Corazón-Guivin5Laboratorio de Microbiología Agrícola “Raúl Ríos Reátegui”, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruLaboratorio de Microbiología Agrícola “Raúl Ríos Reátegui”, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruLaboratorio de Microbiología Agrícola “Raúl Ríos Reátegui”, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruDepartamento Académico de Ingeniería Electrónica, Facultad de Ingeniería Electrónica y Eléctrica, Universidad Nacional Mayor de San Marcos, Lima 15081, PeruLaboratorio de Biología Molecular de Plantas (LBMP), Instituto de Investigación, Innovación y Desarrollo para el Sector Agrario y Agroindustrial (IIDAA), Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, Amazonas, Chachapoyas 01001, PeruLaboratorio de Biología y Genética Molecular, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruThe control of ‘sheath blight’ in rice (<i>Oryza sativa</i> L.), which is caused by the fungus <i>Rhizoctonia oryzae</i>-<i>sativae</i>, has become problematic due to the excessive application of fungicides and their consequent harmful effects. Hence, the search for less contaminating alternatives to conventional chemical products is necessary. This study evaluated the in vitro inhibitory activity of four bacterial strains on the growth of <i>R. oryzae-sativae</i> in both tryptone soy broth (TSB) and mineral medium (MM). The results demonstrated that all evaluated strains (<i>Bacillus tequilensis</i> SMNCT17-02, <i>Priestia aryabhattai</i> SMNCH17-07, <i>Burkholderia vietnamiensis</i> TUR04-01, and <i>Burkholderia vietnamiensis</i> TUR04-03) inhibited the growth of <i>R. oryzae-sativae</i>. Specifically, the activity of <i>B. tequilensis</i> SMNCT17-02 resulted in the smallest area of <i>R. oryzae-sativae</i> growth in both TSB medium (8.54 cm<sup>2</sup>) and MM (5.53 cm<sup>2</sup>), suggesting a notable antifungal effect. When evaluating the action of supernatants generated by the growth of the four bacterial strains in TSB and MM culture media, significant inhibition of <i>R. oryzae-sativae</i> growth was only observed for supernatants produced in MM. This inhibition was attributed to the presence of soluble secondary metabolites. These results offer new perspectives in biotechnology, suggesting the possibility of developing effective products based on easily extractable soluble secondary metabolites, thus promoting sustainable agriculture.https://www.mdpi.com/2037-0164/15/4/70biological controlbiocidesantibiosissoluble secondary metabolites |
| spellingShingle | Liz Cheril Quiñones-Pezo Winston Franz Ríos-Ruiz Danny Fran Pompa-Vásquez Franz Rios-Reategui Angel David Hernández-Amasifuen Mike Anderson Corazón-Guivin In Vitro Inhibition of <i>Rhizoctonia oryzae</i>-<i>sativae</i> Using Bacterial Strains as a Sustainable Alternative for Controlling Sheath Blight in Rice International Journal of Plant Biology biological control biocides antibiosis soluble secondary metabolites |
| title | In Vitro Inhibition of <i>Rhizoctonia oryzae</i>-<i>sativae</i> Using Bacterial Strains as a Sustainable Alternative for Controlling Sheath Blight in Rice |
| title_full | In Vitro Inhibition of <i>Rhizoctonia oryzae</i>-<i>sativae</i> Using Bacterial Strains as a Sustainable Alternative for Controlling Sheath Blight in Rice |
| title_fullStr | In Vitro Inhibition of <i>Rhizoctonia oryzae</i>-<i>sativae</i> Using Bacterial Strains as a Sustainable Alternative for Controlling Sheath Blight in Rice |
| title_full_unstemmed | In Vitro Inhibition of <i>Rhizoctonia oryzae</i>-<i>sativae</i> Using Bacterial Strains as a Sustainable Alternative for Controlling Sheath Blight in Rice |
| title_short | In Vitro Inhibition of <i>Rhizoctonia oryzae</i>-<i>sativae</i> Using Bacterial Strains as a Sustainable Alternative for Controlling Sheath Blight in Rice |
| title_sort | in vitro inhibition of i rhizoctonia oryzae i i sativae i using bacterial strains as a sustainable alternative for controlling sheath blight in rice |
| topic | biological control biocides antibiosis soluble secondary metabolites |
| url | https://www.mdpi.com/2037-0164/15/4/70 |
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