Impact of plant-based nanoparticles synthesized from Carica papaya and Bryophyllum pinnatum against selected microorganisms
Plant-based nanoparticles offer sustainable, eco-friendly alternatives to conventional methods, promising antibacterial properties in the face of antibiotic resistance and addressing global health concerns. Five urine and stool samples were collected from the Benin Medical Centre in Benin City, Edo...
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Cluj University Press
2024-06-01
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| Series: | Studia Universitatis Babeș-Bolyai. Biologia |
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| Online Access: | https://studiabiologia.reviste.ubbcluj.ro/index.php/studiabio/article/view/158 |
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| author | Francis Aibuedefe IGIEBOR Favour Chinaza MICHAEL Ochoyama HARUNA Beckley IKHAJIAGBE |
| author_facet | Francis Aibuedefe IGIEBOR Favour Chinaza MICHAEL Ochoyama HARUNA Beckley IKHAJIAGBE |
| author_sort | Francis Aibuedefe IGIEBOR |
| collection | DOAJ |
| description | Plant-based nanoparticles offer sustainable, eco-friendly alternatives to conventional methods, promising antibacterial properties in the face of antibiotic resistance and addressing global health concerns. Five urine and stool samples were collected from the Benin Medical Centre in Benin City, Edo State, and sent to the Wellspring University Research Laboratory for microbiological analysis. Carica papaya and Bryophyllum pinnatum were used for fresh utilization by washing, weighing, and crushing their leaves, then mixing them with distilled water and heating at 85 °C and 60 °C for 60 minutes. Silver and copper nanoparticles (AgNPs and CuNPs) were synthesized using standard procedures. The NPs were preliminary validated by visual detection of color changes and characterized using a UV-visible spectrophotometer at 300 nm and Fourier transform infrared. The in vitro antimicrobial activity of plant-mediated NPs was investigated using five isolates: S. aureus, B. alvei, H. pylori, P. aeruginosa, and E. coli. The in vitro antimicrobial activity of plant-mediated NPs was investigated using five clinical strains displaying multiple resistance to antibiotics: S. aureus,
B. alvei, H. pylori, P. aeruginosa, and E. coli. The agar-well diffusion method showed inhibition of the isolates by plant-mediated NPs but no inhibition by the plant extract alone. The study indicates that plant-mediated NPs exhibit promising antimicrobial activity, promoting sustainability and eco-friendliness, but further research is needed to assess their safety and efficacy in clinical settings.
Article history: Received 24 November 2023; Revised 19 February 2024;
Accepted 16 May 2024; Available online 30 June 2024. |
| format | Article |
| id | doaj-art-82b03b05e4ad4befa2380ed6d04e798e |
| institution | Kabale University |
| issn | 2065-9512 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Cluj University Press |
| record_format | Article |
| series | Studia Universitatis Babeș-Bolyai. Biologia |
| spelling | doaj-art-82b03b05e4ad4befa2380ed6d04e798e2025-08-20T03:50:38ZengCluj University PressStudia Universitatis Babeș-Bolyai. Biologia2065-95122024-06-018710610.24193/subbbiol.2024.1.04158Impact of plant-based nanoparticles synthesized from Carica papaya and Bryophyllum pinnatum against selected microorganismsFrancis Aibuedefe IGIEBOR0https://orcid.org/0000-0002-2810-8406Favour Chinaza MICHAEL1Ochoyama HARUNA2https://orcid.org/0009-0007-1214-0798Beckley IKHAJIAGBE3https://orcid.org/0000-0003-2834-7447Department of Biological Sciences, College of Science and Computing, Wellspring University, Benin City, Nigeria. Email: francis.igiebor@lifesci.uniben.edu.Department of Biological Sciences, College of Science and Computing, Wellspring University, Benin City, Nigeria. Corresponding author: francis.igiebor@lifesci.uniben.edu.Department of Science Laboratory Technology, Faculty of Life Sciences, University of Benin, Benin City, Nigeria. Corresponding author: francis.igiebor@lifesci.uniben.edu.Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Benin, Benin City, Nigeria. Corresponding author: francis.igiebor@lifesci.uniben.edu.Plant-based nanoparticles offer sustainable, eco-friendly alternatives to conventional methods, promising antibacterial properties in the face of antibiotic resistance and addressing global health concerns. Five urine and stool samples were collected from the Benin Medical Centre in Benin City, Edo State, and sent to the Wellspring University Research Laboratory for microbiological analysis. Carica papaya and Bryophyllum pinnatum were used for fresh utilization by washing, weighing, and crushing their leaves, then mixing them with distilled water and heating at 85 °C and 60 °C for 60 minutes. Silver and copper nanoparticles (AgNPs and CuNPs) were synthesized using standard procedures. The NPs were preliminary validated by visual detection of color changes and characterized using a UV-visible spectrophotometer at 300 nm and Fourier transform infrared. The in vitro antimicrobial activity of plant-mediated NPs was investigated using five isolates: S. aureus, B. alvei, H. pylori, P. aeruginosa, and E. coli. The in vitro antimicrobial activity of plant-mediated NPs was investigated using five clinical strains displaying multiple resistance to antibiotics: S. aureus, B. alvei, H. pylori, P. aeruginosa, and E. coli. The agar-well diffusion method showed inhibition of the isolates by plant-mediated NPs but no inhibition by the plant extract alone. The study indicates that plant-mediated NPs exhibit promising antimicrobial activity, promoting sustainability and eco-friendliness, but further research is needed to assess their safety and efficacy in clinical settings. Article history: Received 24 November 2023; Revised 19 February 2024; Accepted 16 May 2024; Available online 30 June 2024.https://studiabiologia.reviste.ubbcluj.ro/index.php/studiabio/article/view/158nanoparticlesresistantantimicrobialplant-mediatedmar index |
| spellingShingle | Francis Aibuedefe IGIEBOR Favour Chinaza MICHAEL Ochoyama HARUNA Beckley IKHAJIAGBE Impact of plant-based nanoparticles synthesized from Carica papaya and Bryophyllum pinnatum against selected microorganisms Studia Universitatis Babeș-Bolyai. Biologia nanoparticles resistant antimicrobial plant-mediated mar index |
| title | Impact of plant-based nanoparticles synthesized from Carica papaya and Bryophyllum pinnatum against selected microorganisms |
| title_full | Impact of plant-based nanoparticles synthesized from Carica papaya and Bryophyllum pinnatum against selected microorganisms |
| title_fullStr | Impact of plant-based nanoparticles synthesized from Carica papaya and Bryophyllum pinnatum against selected microorganisms |
| title_full_unstemmed | Impact of plant-based nanoparticles synthesized from Carica papaya and Bryophyllum pinnatum against selected microorganisms |
| title_short | Impact of plant-based nanoparticles synthesized from Carica papaya and Bryophyllum pinnatum against selected microorganisms |
| title_sort | impact of plant based nanoparticles synthesized from carica papaya and bryophyllum pinnatum against selected microorganisms |
| topic | nanoparticles resistant antimicrobial plant-mediated mar index |
| url | https://studiabiologia.reviste.ubbcluj.ro/index.php/studiabio/article/view/158 |
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