Effects of osthole on renal tubular epithelial cell injury and Nrf2/HO-1 signal pathway induced by high glucose，hypoxia and reoxygenation
ObjectiveTo explore the effects of osthole on renal tubular epithelial cell damage and Nrf2/HO-1 signal pathway induced by high glucose，hypoxia and reoxygenation.MethodsHuman renal tubular epithelial cells HK-2 were cultured in high-glucose medium and subjected to hypoxia-reoxygenation treatment to...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Clinical Nephrology
2022-05-01
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| Series: | Linchuang shenzangbing zazhi |
| Subjects: | |
| Online Access: | http://www.lcszb.com/thesisDetails#10.3969/j.issn.1671-2390.2022.05.007 |
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| Summary: | ObjectiveTo explore the effects of osthole on renal tubular epithelial cell damage and Nrf2/HO-1 signal pathway induced by high glucose，hypoxia and reoxygenation.MethodsHuman renal tubular epithelial cells HK-2 were cultured in high-glucose medium and subjected to hypoxia-reoxygenation treatment to establish a cellular model.CCK-8 experiment was utilized for detecting the effects of 0，10，20，40，80，160 µg/ml osthole on its growth and screen the optimal concentration.HK-2 cells were divided into control，model，osthole （80 µg/ml），ML385 （Nrf2 inhibitor，20 nmol/ml） and osthole （80 µg/ml）+ML385 （20 nmol/ml） groups.Except for control group，the other groups were induced for establishing cellular models.After high glucose，hypoxia and reoxygenation，CCK-8 experiment was utilized for detecting cellular viability in each group.Kits were employed for measuring the levels of reactive oxygen species （reactive oxygen species，ROS），glutathione peroxidase （GSH-Px），malondialdehyde （MDA），lactate dehydrogenase （LDH），tumor necrosis factor-α （TNF-α），interleukin-6 （IL-6） and interleukin-1β （IL-1β）； Immunofluorescence for detecting the expression of Nrf2/HO-1 pathway protein； Western blot for detecting the expression of Nrf2/HO-1 pathway protein.ResultsCompared with control group，HK-2 cellular viability （100±0 <italic>vs</italic> 43.47±6.14），cellular GSH-Px level （9.53±1.64 <italic>vs</italic> 3.25±0.32） and cellular Nrf2 protein （1.78±0.30 <italic>vs</italic> 0.89±0.12） were markedly down-regulated in model group （all <italic>P</italic><0.05）； the levels of ROS （1.13±0.17 <italic>vs</italic> 6.52±1.03） and TNF-α spiked markedly （all <italic>P</italic><0.05）.Compared with model and osthole + ML385 groups，HK-2 cellular viability （43.47±6.14，44.83±7.06 <italic>vs</italic> 87.76±9.42），cellular GSH-Px level （3.25±0.32，3.27±0.38 <italic>vs</italic> 9.07±1.78） and cellular Nrf2 protein （0.89±0.12，0.90±0.18 <italic>vs</italic> 1.74±0.26） rose in osthole group （<italic>P</italic><0.05）； ROS （6.52±1.03，6.47±0.96 <italic>vs</italic> 1.59±0.21） and TNF-α declined （both <italic>P</italic><0.05）； HK-2 cellular viability （43.47±6.14，44.83±7.06 <italic>vs</italic> 24.80±3.79），cellular GSH-Px level （3.25±0.32，3.27±0.38 <italic>vs</italic> 0.88±0.15） and cellular Nrf2 protein （0.89±0.12，0.90±0.18 <italic>vs</italic> 0.30±0.05） decreased in ML385 group （all <italic>P</italic><0.05）； ROS （6.52±1.03，6.47±0.96 <italic>vs</italic> 9.76±2.02） and TNF-α increased （all <italic>P</italic><0.05）.ConclusionOsthole can up-regulate the expression of Nrf2/HO-1 signaling pathway protein，suppress ROS and inflammatory factors production induced by high glucose，hypoxia and reoxygenation，thereby relieving oxidative stress and inflammation and lessening the damage of renal tubular epithelial cells. |
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| ISSN: | 1671-2390 |