Chip-Scale Aptamer Sandwich Assay Using Optical Waveguide-Assisted Surface-Enhanced Raman Spectroscopy
Chip-scale optical waveguide-assisted surface-enhanced Raman spectroscopy (SERS) that used nanoparticles (NPs) was demonstrated. The Raman signals from Raman reporter (RR) molecules on NPs can be efficiently excited by the waveguide evanescent field when the molecules are in proximity to the wavegui...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/14/23/1927 |
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| author | Megan Makela Dandan Tu Zhihai Lin Gerard Coté Pao Tai Lin |
| author_facet | Megan Makela Dandan Tu Zhihai Lin Gerard Coté Pao Tai Lin |
| author_sort | Megan Makela |
| collection | DOAJ |
| description | Chip-scale optical waveguide-assisted surface-enhanced Raman spectroscopy (SERS) that used nanoparticles (NPs) was demonstrated. The Raman signals from Raman reporter (RR) molecules on NPs can be efficiently excited by the waveguide evanescent field when the molecules are in proximity to the waveguide surface. The Raman signal was enhanced by plasmon resonance due to the NPs close to the waveguide surface. The optical waveguide mode and the NP-induced field enhancement were calculated using a finite difference method (FDM). The sensing performance of the waveguide-assisted SERS device was experimentally characterized by measuring the Raman scattering from various RRs, including 4-mercaptobenzoic acid (4-MBA), 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB), and malachite green isothiocyanate (MGITC). The observed Raman spectral features were identified and assigned to the complex vibrational modes associated with different reporters. A low detection limit of 1 nM was achieved. In addition, the device sensing method was applied to the detection of the biomarker cardiac troponin I (cTnI) using an aptamer sandwich assay immobilized on the device surface. Overall, the optical waveguides integrated with SERS show a miniaturized sensing platform for the detection of small molecules and large proteins, potentially enabling multiplexed detection for clinically relevant applications. |
| format | Article |
| id | doaj-art-82a9076cf7b6423580e978ebfc45fa97 |
| institution | OA Journals |
| issn | 2079-4991 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj-art-82a9076cf7b6423580e978ebfc45fa972025-08-20T01:55:37ZengMDPI AGNanomaterials2079-49912024-11-011423192710.3390/nano14231927Chip-Scale Aptamer Sandwich Assay Using Optical Waveguide-Assisted Surface-Enhanced Raman SpectroscopyMegan Makela0Dandan Tu1Zhihai Lin2Gerard Coté3Pao Tai Lin4Department of Materials Science and Engineering, Texas A&M University, College Station, TX 77843, USACenter for Remote Health Systems and Technologies, Texas A&M University, College Station, TX 77843, USADepartment of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USACenter for Remote Health Systems and Technologies, Texas A&M University, College Station, TX 77843, USADepartment of Materials Science and Engineering, Texas A&M University, College Station, TX 77843, USAChip-scale optical waveguide-assisted surface-enhanced Raman spectroscopy (SERS) that used nanoparticles (NPs) was demonstrated. The Raman signals from Raman reporter (RR) molecules on NPs can be efficiently excited by the waveguide evanescent field when the molecules are in proximity to the waveguide surface. The Raman signal was enhanced by plasmon resonance due to the NPs close to the waveguide surface. The optical waveguide mode and the NP-induced field enhancement were calculated using a finite difference method (FDM). The sensing performance of the waveguide-assisted SERS device was experimentally characterized by measuring the Raman scattering from various RRs, including 4-mercaptobenzoic acid (4-MBA), 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB), and malachite green isothiocyanate (MGITC). The observed Raman spectral features were identified and assigned to the complex vibrational modes associated with different reporters. A low detection limit of 1 nM was achieved. In addition, the device sensing method was applied to the detection of the biomarker cardiac troponin I (cTnI) using an aptamer sandwich assay immobilized on the device surface. Overall, the optical waveguides integrated with SERS show a miniaturized sensing platform for the detection of small molecules and large proteins, potentially enabling multiplexed detection for clinically relevant applications.https://www.mdpi.com/2079-4991/14/23/1927surface-enhanced Raman spectroscopy (SERS)optical waveguidesaptamernanoparticles (NPs)biosensors |
| spellingShingle | Megan Makela Dandan Tu Zhihai Lin Gerard Coté Pao Tai Lin Chip-Scale Aptamer Sandwich Assay Using Optical Waveguide-Assisted Surface-Enhanced Raman Spectroscopy Nanomaterials surface-enhanced Raman spectroscopy (SERS) optical waveguides aptamer nanoparticles (NPs) biosensors |
| title | Chip-Scale Aptamer Sandwich Assay Using Optical Waveguide-Assisted Surface-Enhanced Raman Spectroscopy |
| title_full | Chip-Scale Aptamer Sandwich Assay Using Optical Waveguide-Assisted Surface-Enhanced Raman Spectroscopy |
| title_fullStr | Chip-Scale Aptamer Sandwich Assay Using Optical Waveguide-Assisted Surface-Enhanced Raman Spectroscopy |
| title_full_unstemmed | Chip-Scale Aptamer Sandwich Assay Using Optical Waveguide-Assisted Surface-Enhanced Raman Spectroscopy |
| title_short | Chip-Scale Aptamer Sandwich Assay Using Optical Waveguide-Assisted Surface-Enhanced Raman Spectroscopy |
| title_sort | chip scale aptamer sandwich assay using optical waveguide assisted surface enhanced raman spectroscopy |
| topic | surface-enhanced Raman spectroscopy (SERS) optical waveguides aptamer nanoparticles (NPs) biosensors |
| url | https://www.mdpi.com/2079-4991/14/23/1927 |
| work_keys_str_mv | AT meganmakela chipscaleaptamersandwichassayusingopticalwaveguideassistedsurfaceenhancedramanspectroscopy AT dandantu chipscaleaptamersandwichassayusingopticalwaveguideassistedsurfaceenhancedramanspectroscopy AT zhihailin chipscaleaptamersandwichassayusingopticalwaveguideassistedsurfaceenhancedramanspectroscopy AT gerardcote chipscaleaptamersandwichassayusingopticalwaveguideassistedsurfaceenhancedramanspectroscopy AT paotailin chipscaleaptamersandwichassayusingopticalwaveguideassistedsurfaceenhancedramanspectroscopy |