INCREASE OF BARLEY TOLERANCE TO TOXICITY OF METALS AND OSMOTIC STRESS USING CELL SELECTION
The reaction of barley in callus cultures has been studied using ions Cd2+, Mn2+, Al3+ and PEG as osmotic. Analyzing amount of doses for crop survival, lethal and sublethal metal concentrates were found: manganese (350 and 250 mg/l, respectively), cadmium (30 and 10 mg/l), aluminum (42 and 20 mg/l)...
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| Format: | Article |
| Language: | Russian |
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Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"
2018-05-01
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| Series: | Зерновое хозяйство России |
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| Online Access: | https://www.zhros.online/jour/article/view/231 |
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| author | O. N. Shupletsova I. G. Shirokikh |
| author_facet | O. N. Shupletsova I. G. Shirokikh |
| author_sort | O. N. Shupletsova |
| collection | DOAJ |
| description | The reaction of barley in callus cultures has been studied using ions Cd2+, Mn2+, Al3+ and PEG as osmotic. Analyzing amount of doses for crop survival, lethal and sublethal metal concentrates were found: manganese (350 and 250 mg/l, respectively), cadmium (30 and 10 mg/l), aluminum (42 and 20 mg/l). Optimal schemes have been suggested to select callus lines tolerant to a complex of stresses. To select a line resistant to complex ion toxicity, it has been recommended to use ions of cadmium (20 mg/l) during a proliferation period and ions of manganese (200 mg/l) during a period of morphogenesis. Ions of aluminum (20-30 mg/l) can be used during both stages of callus tissue development, supporting survival of callus and frequency of plant regeneration. To choose callus with total tolerance to ion toxicity and osmotic stress it’s advisable to apply osmotic (10% of PEG) only during a period of morphogenesis, during a proliferation period it’s possible to use aluminum (20 mg/l) with ions of cadmium (20 mg/l) or ions of manganese (200 mg/l). Using the developed scheme of selection we received 50 plants-regenerators of barley for applying them as starting material in crop selection. |
| format | Article |
| id | doaj-art-82a736dceedf4e60ba86d146190436ab |
| institution | Kabale University |
| issn | 2079-8725 2079-8733 |
| language | Russian |
| publishDate | 2018-05-01 |
| publisher | Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”" |
| record_format | Article |
| series | Зерновое хозяйство России |
| spelling | doaj-art-82a736dceedf4e60ba86d146190436ab2025-08-20T03:57:27ZrusFederal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"Зерновое хозяйство России2079-87252079-87332018-05-0101124135230INCREASE OF BARLEY TOLERANCE TO TOXICITY OF METALS AND OSMOTIC STRESS USING CELL SELECTIONO. N. Shupletsova0I. G. Shirokikh1FSBRI “Research Institute of Agriculture of North-East Area after N.V. Rudnitsky”FSBRI “Research Institute of Agriculture of North-East Area after N.V. Rudnitsky”The reaction of barley in callus cultures has been studied using ions Cd2+, Mn2+, Al3+ and PEG as osmotic. Analyzing amount of doses for crop survival, lethal and sublethal metal concentrates were found: manganese (350 and 250 mg/l, respectively), cadmium (30 and 10 mg/l), aluminum (42 and 20 mg/l). Optimal schemes have been suggested to select callus lines tolerant to a complex of stresses. To select a line resistant to complex ion toxicity, it has been recommended to use ions of cadmium (20 mg/l) during a proliferation period and ions of manganese (200 mg/l) during a period of morphogenesis. Ions of aluminum (20-30 mg/l) can be used during both stages of callus tissue development, supporting survival of callus and frequency of plant regeneration. To choose callus with total tolerance to ion toxicity and osmotic stress it’s advisable to apply osmotic (10% of PEG) only during a period of morphogenesis, during a proliferation period it’s possible to use aluminum (20 mg/l) with ions of cadmium (20 mg/l) or ions of manganese (200 mg/l). Using the developed scheme of selection we received 50 plants-regenerators of barley for applying them as starting material in crop selection.https://www.zhros.online/jour/article/view/231barley (hordeum vulgare l.)callus cropaluminumcadmiummanganesewater deficitschemes of selectionplant-regenerators |
| spellingShingle | O. N. Shupletsova I. G. Shirokikh INCREASE OF BARLEY TOLERANCE TO TOXICITY OF METALS AND OSMOTIC STRESS USING CELL SELECTION Зерновое хозяйство России barley (hordeum vulgare l.) callus crop aluminum cadmium manganese water deficit schemes of selection plant-regenerators |
| title | INCREASE OF BARLEY TOLERANCE TO TOXICITY OF METALS AND OSMOTIC STRESS USING CELL SELECTION |
| title_full | INCREASE OF BARLEY TOLERANCE TO TOXICITY OF METALS AND OSMOTIC STRESS USING CELL SELECTION |
| title_fullStr | INCREASE OF BARLEY TOLERANCE TO TOXICITY OF METALS AND OSMOTIC STRESS USING CELL SELECTION |
| title_full_unstemmed | INCREASE OF BARLEY TOLERANCE TO TOXICITY OF METALS AND OSMOTIC STRESS USING CELL SELECTION |
| title_short | INCREASE OF BARLEY TOLERANCE TO TOXICITY OF METALS AND OSMOTIC STRESS USING CELL SELECTION |
| title_sort | increase of barley tolerance to toxicity of metals and osmotic stress using cell selection |
| topic | barley (hordeum vulgare l.) callus crop aluminum cadmium manganese water deficit schemes of selection plant-regenerators |
| url | https://www.zhros.online/jour/article/view/231 |
| work_keys_str_mv | AT onshupletsova increaseofbarleytolerancetotoxicityofmetalsandosmoticstressusingcellselection AT igshirokikh increaseofbarleytolerancetotoxicityofmetalsandosmoticstressusingcellselection |