The Influence of Sperm Activation Methods and Oocyte Collection on the Reproductive Effects of Northern Pike (<i>Esox lucius</i>)
This study compared the quality of northern pike eggs collected using traditional methods (hand stripping) and pneumatic methods (air stripping). The effects of different activation solutions (0.4% NaCl, 0.8% NaCl, and hatchery water) on egg fertilization under controlled conditions were also invest...
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MDPI AG
2024-12-01
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| author | Michał Cydzik Krystyna Demska-Zakęś Mirosław Szczepkowski Bożena Szczepkowska Beata Sarosiek Michał Blitek Aleksy Kowalski Radosław Kajetan Kowalski |
| author_facet | Michał Cydzik Krystyna Demska-Zakęś Mirosław Szczepkowski Bożena Szczepkowska Beata Sarosiek Michał Blitek Aleksy Kowalski Radosław Kajetan Kowalski |
| author_sort | Michał Cydzik |
| collection | DOAJ |
| description | This study compared the quality of northern pike eggs collected using traditional methods (hand stripping) and pneumatic methods (air stripping). The effects of different activation solutions (0.4% NaCl, 0.8% NaCl, and hatchery water) on egg fertilization under controlled conditions were also investigated. After egg collection, the Pseudo-Gonado-Somatic Index (PGSI) was measured; the PGSI values in the samples obtained using the pneumatic method (13.8 ± 3.9%) were lower, but did not differ statistically from those obtained by hand stripping (16.5 ± 5.4%). The 0.4% NaCl solution proved to be the most effective for sperm activation, as assessed by the Computer-Assisted Sperm Analysis (CASA) system, compared to the 0.8% NaCl solution. The pneumatic method achieved a higher egg collection efficiency (93.7% occlusion) than the traditional method, with significant differences observed in groups activated with water. The average hatching percentage of larvae was 89.5% in groups using the pneumatic method, compared to 71.2% in the traditional groups, highlighting the advantages of this modern approach. The application of the pneumatic method and 0.4% NaCl for the artificial fertilization of northern pike resulted in higher fertilization and hatching rates compared to other techniques, making this method a promising option for the artificial reproduction of other fish species. |
| format | Article |
| id | doaj-art-82a17537c9e64908ad9efd47ba9149d0 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-82a17537c9e64908ad9efd47ba9149d02025-01-10T13:13:46ZengMDPI AGAnimals2076-26152024-12-01151810.3390/ani15010008The Influence of Sperm Activation Methods and Oocyte Collection on the Reproductive Effects of Northern Pike (<i>Esox lucius</i>)Michał Cydzik0Krystyna Demska-Zakęś1Mirosław Szczepkowski2Bożena Szczepkowska3Beata Sarosiek4Michał Blitek5Aleksy Kowalski6Radosław Kajetan Kowalski7Department of Aquaculture, National Inland Fisheries Research Institute, 10-719 Olsztyn, PolandDepartment of Ichthyology and Aquaculture, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandDepartment of Sturgeon Fish Breeding, National Inland Fisheries Research Institute, 10-719 Olsztyn, PolandDepartment of Sturgeon Fish Breeding, National Inland Fisheries Research Institute, 10-719 Olsztyn, PolandDepartment of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-243 Olsztyn, PolandDepartment of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-243 Olsztyn, PolandFaculty of Medicine, Medical University of Bialystok; 15-089, Bialystok, PolandDepartment of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-243 Olsztyn, PolandThis study compared the quality of northern pike eggs collected using traditional methods (hand stripping) and pneumatic methods (air stripping). The effects of different activation solutions (0.4% NaCl, 0.8% NaCl, and hatchery water) on egg fertilization under controlled conditions were also investigated. After egg collection, the Pseudo-Gonado-Somatic Index (PGSI) was measured; the PGSI values in the samples obtained using the pneumatic method (13.8 ± 3.9%) were lower, but did not differ statistically from those obtained by hand stripping (16.5 ± 5.4%). The 0.4% NaCl solution proved to be the most effective for sperm activation, as assessed by the Computer-Assisted Sperm Analysis (CASA) system, compared to the 0.8% NaCl solution. The pneumatic method achieved a higher egg collection efficiency (93.7% occlusion) than the traditional method, with significant differences observed in groups activated with water. The average hatching percentage of larvae was 89.5% in groups using the pneumatic method, compared to 71.2% in the traditional groups, highlighting the advantages of this modern approach. The application of the pneumatic method and 0.4% NaCl for the artificial fertilization of northern pike resulted in higher fertilization and hatching rates compared to other techniques, making this method a promising option for the artificial reproduction of other fish species.https://www.mdpi.com/2076-2615/15/1/8pikeeggsair strippingsodium chloridespermfertilization |
| spellingShingle | Michał Cydzik Krystyna Demska-Zakęś Mirosław Szczepkowski Bożena Szczepkowska Beata Sarosiek Michał Blitek Aleksy Kowalski Radosław Kajetan Kowalski The Influence of Sperm Activation Methods and Oocyte Collection on the Reproductive Effects of Northern Pike (<i>Esox lucius</i>) Animals pike eggs air stripping sodium chloride sperm fertilization |
| title | The Influence of Sperm Activation Methods and Oocyte Collection on the Reproductive Effects of Northern Pike (<i>Esox lucius</i>) |
| title_full | The Influence of Sperm Activation Methods and Oocyte Collection on the Reproductive Effects of Northern Pike (<i>Esox lucius</i>) |
| title_fullStr | The Influence of Sperm Activation Methods and Oocyte Collection on the Reproductive Effects of Northern Pike (<i>Esox lucius</i>) |
| title_full_unstemmed | The Influence of Sperm Activation Methods and Oocyte Collection on the Reproductive Effects of Northern Pike (<i>Esox lucius</i>) |
| title_short | The Influence of Sperm Activation Methods and Oocyte Collection on the Reproductive Effects of Northern Pike (<i>Esox lucius</i>) |
| title_sort | influence of sperm activation methods and oocyte collection on the reproductive effects of northern pike i esox lucius i |
| topic | pike eggs air stripping sodium chloride sperm fertilization |
| url | https://www.mdpi.com/2076-2615/15/1/8 |
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