Mediator effect of luteolin on electrooxidation of NADH
The effects of luteolin (LU) as a new mediator on the electrooxidation of the reduced nicotinamide adenine dinucleotide (NADH) were investigated and developed a new disposable NADH sensor. Firstly, screen printed carbon electrodes (SPCE) modified with gold nanoparticles (AuNP), and then, LU was depo...
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Sakarya University
2021-04-01
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| Series: | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1127304 |
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| author | Gulshat Gylyjova Melike Bilgi Kamaç |
| author_facet | Gulshat Gylyjova Melike Bilgi Kamaç |
| author_sort | Gulshat Gylyjova |
| collection | DOAJ |
| description | The effects of luteolin (LU) as a new mediator on the electrooxidation of the reduced nicotinamide adenine dinucleotide (NADH) were investigated and developed a new disposable NADH sensor. Firstly, screen printed carbon electrodes (SPCE) modified with gold nanoparticles (AuNP), and then, LU was deposited on SPCE modified with AuNP (SPCE/AuNP) using cyclic voltammetry. Electrochemical behavior of LU on SPCE/AuNP was investigated and, the redox proses of LU on SPCE/AuNP was found to be adsorption controlled. The number of cycles was optimized for the electrochemical deposition of LU and determined to be 15 cycles. LU modified SPCE/AuNP (SPCE/AuNP/LU) was found to show the electrocatalytic effect on electrooxidation of NADH and acts as a mediator. Working potential and pH were optimized for electrochemical detection of NADH with the proposed SPCE/AuNP/LU sensor and found to be +0.225 V and 7.25, respectively. The proposed NADH sensor showed a well linear response from 15.90 to 925 μM with a detection limit of 4.57 μM and a sensitivity of 11.19 μAmM−1. The repeatability of the NADH sensor was tested +0.225 V with 50 µM NADH solution. The relative standard deviation (RSD) was calculated as 3.68% (for n=10). The operational stability studies have shown that the initial amperometric response of sensor to NADH decreased by 62.1% at the 30th day. The analysis of NADH in artificial human serum samples was successfully performed with SPCE/AuNP/LU sensor. |
| format | Article |
| id | doaj-art-829ea768673349998f3f36baaeb83114 |
| institution | DOAJ |
| issn | 2147-835X |
| language | English |
| publishDate | 2021-04-01 |
| publisher | Sakarya University |
| record_format | Article |
| series | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| spelling | doaj-art-829ea768673349998f3f36baaeb831142025-08-20T02:40:27ZengSakarya UniversitySakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi2147-835X2021-04-0125245346510.16984/saufenbilder.74561028Mediator effect of luteolin on electrooxidation of NADHGulshat Gylyjova0https://orcid.org/0000-0002-8798-9119Melike Bilgi Kamaç1https://orcid.org/0000-0002-3381-7522ÇANKIRI KARATEKİN ÜNİVERSİTESİÇANKIRI KARATEKİN ÜNİVERSİTESİThe effects of luteolin (LU) as a new mediator on the electrooxidation of the reduced nicotinamide adenine dinucleotide (NADH) were investigated and developed a new disposable NADH sensor. Firstly, screen printed carbon electrodes (SPCE) modified with gold nanoparticles (AuNP), and then, LU was deposited on SPCE modified with AuNP (SPCE/AuNP) using cyclic voltammetry. Electrochemical behavior of LU on SPCE/AuNP was investigated and, the redox proses of LU on SPCE/AuNP was found to be adsorption controlled. The number of cycles was optimized for the electrochemical deposition of LU and determined to be 15 cycles. LU modified SPCE/AuNP (SPCE/AuNP/LU) was found to show the electrocatalytic effect on electrooxidation of NADH and acts as a mediator. Working potential and pH were optimized for electrochemical detection of NADH with the proposed SPCE/AuNP/LU sensor and found to be +0.225 V and 7.25, respectively. The proposed NADH sensor showed a well linear response from 15.90 to 925 μM with a detection limit of 4.57 μM and a sensitivity of 11.19 μAmM−1. The repeatability of the NADH sensor was tested +0.225 V with 50 µM NADH solution. The relative standard deviation (RSD) was calculated as 3.68% (for n=10). The operational stability studies have shown that the initial amperometric response of sensor to NADH decreased by 62.1% at the 30th day. The analysis of NADH in artificial human serum samples was successfully performed with SPCE/AuNP/LU sensor.https://dergipark.org.tr/tr/download/article-file/1127304nadhluteolingold nanoparticlesredox mediatorhuman blood serum |
| spellingShingle | Gulshat Gylyjova Melike Bilgi Kamaç Mediator effect of luteolin on electrooxidation of NADH Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi nadh luteolin gold nanoparticles redox mediator human blood serum |
| title | Mediator effect of luteolin on electrooxidation of NADH |
| title_full | Mediator effect of luteolin on electrooxidation of NADH |
| title_fullStr | Mediator effect of luteolin on electrooxidation of NADH |
| title_full_unstemmed | Mediator effect of luteolin on electrooxidation of NADH |
| title_short | Mediator effect of luteolin on electrooxidation of NADH |
| title_sort | mediator effect of luteolin on electrooxidation of nadh |
| topic | nadh luteolin gold nanoparticles redox mediator human blood serum |
| url | https://dergipark.org.tr/tr/download/article-file/1127304 |
| work_keys_str_mv | AT gulshatgylyjova mediatoreffectofluteolinonelectrooxidationofnadh AT melikebilgikamac mediatoreffectofluteolinonelectrooxidationofnadh |