Premature termination codon: a tunable protein translation approach
Cellular protein–protein interactions are largely dependent on the activities of signaling proteins. Here, we present a technique to tune gene expression at translation level based on G418-inducible readthrough premature termination codon (PTC-on). To demonstrate how this PTC-on can control the expr...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-08-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2022-0046 |
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| author | Xiyao Cheng Ting Zhou Zixin Yang Jingjing Zhou Meng Gao Yongqi Huang Zhengding Su |
| author_facet | Xiyao Cheng Ting Zhou Zixin Yang Jingjing Zhou Meng Gao Yongqi Huang Zhengding Su |
| author_sort | Xiyao Cheng |
| collection | DOAJ |
| description | Cellular protein–protein interactions are largely dependent on the activities of signaling proteins. Here, we present a technique to tune gene expression at translation level based on G418-inducible readthrough premature termination codon (PTC-on). To demonstrate how this PTC-on can control the expression level of a cellular signaling protein to regulate signal transduction, we settled a p53 PTC-on system in p53-null H1299 cells. After treating with G418, the cells expressed full-length p53 protein in a dose-dependent manner. We further demonstrated to use this PTC-on approach to dissect p53-dependent and p53-independent apoptosis in response to the DNA double strand breaks in H1299 cells. In principle, the PTC-on can be used as a general approach for exploring the functions of any other signaling proteins. |
| format | Article |
| id | doaj-art-829a8aae5f3b47078ff904973b24c79e |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-829a8aae5f3b47078ff904973b24c79e2025-08-20T02:25:34ZengTaylor & Francis GroupBioTechniques0736-62051940-98182022-08-01732808910.2144/btn-2022-0046Premature termination codon: a tunable protein translation approachXiyao Cheng0Ting Zhou1Zixin Yang2Jingjing Zhou3Meng Gao4Yongqi Huang5Zhengding Su61School of Light Industry and Food Engineering, Guanxi University, No. 100, Daxuedong Road, Xixiangtang District, Nanning, Guangxi, 530004, China2Protein Engineering & Biopharmaceutical Sciences Laboratory, Hubei University of Technology, 28 Nanli Road, Wuhan, Hubei, 430068, China2Protein Engineering & Biopharmaceutical Sciences Laboratory, Hubei University of Technology, 28 Nanli Road, Wuhan, Hubei, 430068, China2Protein Engineering & Biopharmaceutical Sciences Laboratory, Hubei University of Technology, 28 Nanli Road, Wuhan, Hubei, 430068, China2Protein Engineering & Biopharmaceutical Sciences Laboratory, Hubei University of Technology, 28 Nanli Road, Wuhan, Hubei, 430068, China2Protein Engineering & Biopharmaceutical Sciences Laboratory, Hubei University of Technology, 28 Nanli Road, Wuhan, Hubei, 430068, China2Protein Engineering & Biopharmaceutical Sciences Laboratory, Hubei University of Technology, 28 Nanli Road, Wuhan, Hubei, 430068, ChinaCellular protein–protein interactions are largely dependent on the activities of signaling proteins. Here, we present a technique to tune gene expression at translation level based on G418-inducible readthrough premature termination codon (PTC-on). To demonstrate how this PTC-on can control the expression level of a cellular signaling protein to regulate signal transduction, we settled a p53 PTC-on system in p53-null H1299 cells. After treating with G418, the cells expressed full-length p53 protein in a dose-dependent manner. We further demonstrated to use this PTC-on approach to dissect p53-dependent and p53-independent apoptosis in response to the DNA double strand breaks in H1299 cells. In principle, the PTC-on can be used as a general approach for exploring the functions of any other signaling proteins.https://www.future-science.com/doi/10.2144/btn-2022-0046apoptosiscell cycle arrestgene expressionMMSp53premature termination codon |
| spellingShingle | Xiyao Cheng Ting Zhou Zixin Yang Jingjing Zhou Meng Gao Yongqi Huang Zhengding Su Premature termination codon: a tunable protein translation approach BioTechniques apoptosis cell cycle arrest gene expression MMS p53 premature termination codon |
| title | Premature termination codon: a tunable protein translation approach |
| title_full | Premature termination codon: a tunable protein translation approach |
| title_fullStr | Premature termination codon: a tunable protein translation approach |
| title_full_unstemmed | Premature termination codon: a tunable protein translation approach |
| title_short | Premature termination codon: a tunable protein translation approach |
| title_sort | premature termination codon a tunable protein translation approach |
| topic | apoptosis cell cycle arrest gene expression MMS p53 premature termination codon |
| url | https://www.future-science.com/doi/10.2144/btn-2022-0046 |
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