A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup
A simple reversed phase high-performance liquid chromatographic (RP-HPLC) method coupled with a photodiode array detector (PAD) has been developed and validated for the analysis of hederacoside C, the marker of ivy plant, in Ivy-Thyme cough syrup. Separation of hederacoside C was achieved using a Ph...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/478143 |
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| author | Ayman Khdair Mohammad K. Mohammad Khaled Tawaha Eman Al-Hamarsheh Hatim S. AlKhatib Bashar Al-khalidi Yasser Bustanji Samer Najjar Mohammad Hudaib |
| author_facet | Ayman Khdair Mohammad K. Mohammad Khaled Tawaha Eman Al-Hamarsheh Hatim S. AlKhatib Bashar Al-khalidi Yasser Bustanji Samer Najjar Mohammad Hudaib |
| author_sort | Ayman Khdair |
| collection | DOAJ |
| description | A simple reversed phase high-performance liquid chromatographic (RP-HPLC) method coupled with a photodiode array detector (PAD) has been developed and validated for the analysis of hederacoside C, the marker of ivy plant, in Ivy-Thyme cough syrup. Separation of hederacoside C was achieved using a Phenomenex-Gemini C18 column isothermally at 40∘C. A mobile phase system constituted of solvent A (water: acetonitrile: orthophosphoric acid (85%), 860 : 140 : 2 v/v) and solvent B (acetonitrile: orthophosphoric acid (85%), 998 : 2 v/v) was used, at gradient conditions, at a flow rate of 1.5 mL/min. Analysis was performed using UV-detection (205 nm). The method was linear over the range (0.03–0.15) mg/mL of hederacoside C (𝑟=0.9992). Repeatability and intermediate precision were acceptable (RSD <2%). Limits of detection (LOD) and quantitation (LOQ) were 0.011 and 0.032 mg/mL, respectively. Percentage recovery was found to lie between 99.69% and 100.90% (RSD <2%). The method was also proved to be specific (peak-purity coefficient=0.996). |
| format | Article |
| id | doaj-art-8296d1db17c24934b85ded0e6bcd327f |
| institution | OA Journals |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-8296d1db17c24934b85ded0e6bcd327f2025-08-20T02:08:31ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792010-01-01201010.1155/2010/478143478143A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough SyrupAyman Khdair0Mohammad K. Mohammad1Khaled Tawaha2Eman Al-Hamarsheh3Hatim S. AlKhatib4Bashar Al-khalidi5Yasser Bustanji6Samer Najjar7Mohammad Hudaib8Faculty of Pharmacy, Applied Sciences University, Amman 11931, JordanDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan (UJ), Amman 11942, JordanDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan (UJ), Amman 11942, JordanDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan (UJ), Amman 11942, JordanDepartment of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Jordan (UJ), Amman 11942, JordanDepartment of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Jordan (UJ), Amman 11942, JordanDepartment of Clinical Pharmacy and Biopharmaceutics, Faculty of Pharmacy, University of Jordan (UJ), Amman 11942, JordanSana Pharmaceutical Research Co., Amman 11931, JordanDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan (UJ), Amman 11942, JordanA simple reversed phase high-performance liquid chromatographic (RP-HPLC) method coupled with a photodiode array detector (PAD) has been developed and validated for the analysis of hederacoside C, the marker of ivy plant, in Ivy-Thyme cough syrup. Separation of hederacoside C was achieved using a Phenomenex-Gemini C18 column isothermally at 40∘C. A mobile phase system constituted of solvent A (water: acetonitrile: orthophosphoric acid (85%), 860 : 140 : 2 v/v) and solvent B (acetonitrile: orthophosphoric acid (85%), 998 : 2 v/v) was used, at gradient conditions, at a flow rate of 1.5 mL/min. Analysis was performed using UV-detection (205 nm). The method was linear over the range (0.03–0.15) mg/mL of hederacoside C (𝑟=0.9992). Repeatability and intermediate precision were acceptable (RSD <2%). Limits of detection (LOD) and quantitation (LOQ) were 0.011 and 0.032 mg/mL, respectively. Percentage recovery was found to lie between 99.69% and 100.90% (RSD <2%). The method was also proved to be specific (peak-purity coefficient=0.996).http://dx.doi.org/10.1155/2010/478143 |
| spellingShingle | Ayman Khdair Mohammad K. Mohammad Khaled Tawaha Eman Al-Hamarsheh Hatim S. AlKhatib Bashar Al-khalidi Yasser Bustanji Samer Najjar Mohammad Hudaib A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup International Journal of Analytical Chemistry |
| title | A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup |
| title_full | A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup |
| title_fullStr | A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup |
| title_full_unstemmed | A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup |
| title_short | A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup |
| title_sort | validated rp hplc pad method for the determination of hederacoside c in ivy thyme cough syrup |
| url | http://dx.doi.org/10.1155/2010/478143 |
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