Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure
Mesenchymal stem cells (MSCs) have shown much promise with respect to their use in cartilage tissue engineering. MSCs can be obtained from many different tissue sources. Among these, adipose tissue can provide an abundant source of adipose-derived mesenchymal stem cells (ADMSCs). The infrapatellar f...
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Format: | Article |
Language: | English |
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Wiley
2018-01-01
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Series: | Stem Cells International |
Online Access: | http://dx.doi.org/10.1155/2018/8947548 |
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author | Sam L. Francis Serena Duchi Carmine Onofrillo Claudia Di Bella Peter F. M. Choong |
author_facet | Sam L. Francis Serena Duchi Carmine Onofrillo Claudia Di Bella Peter F. M. Choong |
author_sort | Sam L. Francis |
collection | DOAJ |
description | Mesenchymal stem cells (MSCs) have shown much promise with respect to their use in cartilage tissue engineering. MSCs can be obtained from many different tissue sources. Among these, adipose tissue can provide an abundant source of adipose-derived mesenchymal stem cells (ADMSCs). The infrapatellar fat pad (IFP) is a promising source of ADMSCs with respect to producing a cartilage lineage. Cell isolation protocols to date are time-consuming and follow conservative approaches that rely on a long incubation period of 24–48 hours. The different types of ADMSC isolation techniques used for cartilage repair will be reviewed and compared with the view of developing a rapid one-step isolation protocol that can be applied in the context of a surgical procedure. |
format | Article |
id | doaj-art-828aa96a6fe44d4da9980ce89ee03883 |
institution | Kabale University |
issn | 1687-966X 1687-9678 |
language | English |
publishDate | 2018-01-01 |
publisher | Wiley |
record_format | Article |
series | Stem Cells International |
spelling | doaj-art-828aa96a6fe44d4da9980ce89ee038832025-02-03T05:44:23ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/89475488947548Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation ProcedureSam L. Francis0Serena Duchi1Carmine Onofrillo2Claudia Di Bella3Peter F. M. Choong4Department of Surgery, The University of Melbourne, Melbourne, VIC, AustraliaDepartment of Surgery, The University of Melbourne, Melbourne, VIC, AustraliaDepartment of Surgery, The University of Melbourne, Melbourne, VIC, AustraliaDepartment of Surgery, The University of Melbourne, Melbourne, VIC, AustraliaDepartment of Surgery, The University of Melbourne, Melbourne, VIC, AustraliaMesenchymal stem cells (MSCs) have shown much promise with respect to their use in cartilage tissue engineering. MSCs can be obtained from many different tissue sources. Among these, adipose tissue can provide an abundant source of adipose-derived mesenchymal stem cells (ADMSCs). The infrapatellar fat pad (IFP) is a promising source of ADMSCs with respect to producing a cartilage lineage. Cell isolation protocols to date are time-consuming and follow conservative approaches that rely on a long incubation period of 24–48 hours. The different types of ADMSC isolation techniques used for cartilage repair will be reviewed and compared with the view of developing a rapid one-step isolation protocol that can be applied in the context of a surgical procedure.http://dx.doi.org/10.1155/2018/8947548 |
spellingShingle | Sam L. Francis Serena Duchi Carmine Onofrillo Claudia Di Bella Peter F. M. Choong Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure Stem Cells International |
title | Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure |
title_full | Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure |
title_fullStr | Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure |
title_full_unstemmed | Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure |
title_short | Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure |
title_sort | adipose derived mesenchymal stem cells in the use of cartilage tissue engineering the need for a rapid isolation procedure |
url | http://dx.doi.org/10.1155/2018/8947548 |
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