Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure

Mesenchymal stem cells (MSCs) have shown much promise with respect to their use in cartilage tissue engineering. MSCs can be obtained from many different tissue sources. Among these, adipose tissue can provide an abundant source of adipose-derived mesenchymal stem cells (ADMSCs). The infrapatellar f...

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Main Authors: Sam L. Francis, Serena Duchi, Carmine Onofrillo, Claudia Di Bella, Peter F. M. Choong
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2018/8947548
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author Sam L. Francis
Serena Duchi
Carmine Onofrillo
Claudia Di Bella
Peter F. M. Choong
author_facet Sam L. Francis
Serena Duchi
Carmine Onofrillo
Claudia Di Bella
Peter F. M. Choong
author_sort Sam L. Francis
collection DOAJ
description Mesenchymal stem cells (MSCs) have shown much promise with respect to their use in cartilage tissue engineering. MSCs can be obtained from many different tissue sources. Among these, adipose tissue can provide an abundant source of adipose-derived mesenchymal stem cells (ADMSCs). The infrapatellar fat pad (IFP) is a promising source of ADMSCs with respect to producing a cartilage lineage. Cell isolation protocols to date are time-consuming and follow conservative approaches that rely on a long incubation period of 24–48 hours. The different types of ADMSC isolation techniques used for cartilage repair will be reviewed and compared with the view of developing a rapid one-step isolation protocol that can be applied in the context of a surgical procedure.
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institution Kabale University
issn 1687-966X
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spelling doaj-art-828aa96a6fe44d4da9980ce89ee038832025-02-03T05:44:23ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/89475488947548Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation ProcedureSam L. Francis0Serena Duchi1Carmine Onofrillo2Claudia Di Bella3Peter F. M. Choong4Department of Surgery, The University of Melbourne, Melbourne, VIC, AustraliaDepartment of Surgery, The University of Melbourne, Melbourne, VIC, AustraliaDepartment of Surgery, The University of Melbourne, Melbourne, VIC, AustraliaDepartment of Surgery, The University of Melbourne, Melbourne, VIC, AustraliaDepartment of Surgery, The University of Melbourne, Melbourne, VIC, AustraliaMesenchymal stem cells (MSCs) have shown much promise with respect to their use in cartilage tissue engineering. MSCs can be obtained from many different tissue sources. Among these, adipose tissue can provide an abundant source of adipose-derived mesenchymal stem cells (ADMSCs). The infrapatellar fat pad (IFP) is a promising source of ADMSCs with respect to producing a cartilage lineage. Cell isolation protocols to date are time-consuming and follow conservative approaches that rely on a long incubation period of 24–48 hours. The different types of ADMSC isolation techniques used for cartilage repair will be reviewed and compared with the view of developing a rapid one-step isolation protocol that can be applied in the context of a surgical procedure.http://dx.doi.org/10.1155/2018/8947548
spellingShingle Sam L. Francis
Serena Duchi
Carmine Onofrillo
Claudia Di Bella
Peter F. M. Choong
Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure
Stem Cells International
title Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure
title_full Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure
title_fullStr Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure
title_full_unstemmed Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure
title_short Adipose-Derived Mesenchymal Stem Cells in the Use of Cartilage Tissue Engineering: The Need for a Rapid Isolation Procedure
title_sort adipose derived mesenchymal stem cells in the use of cartilage tissue engineering the need for a rapid isolation procedure
url http://dx.doi.org/10.1155/2018/8947548
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