Effect of Electroacupuncture on Rats with Chronic Constriction Injury-Induced Neuropathic Pain
We adopt the chronic constriction injury (CCI) model to induce neuropathic pain to Spragrue-Dawley (SD) rats by ligating the right sciatic nerve of using four 4-0 chromic gut sutures and subsequently applying 2 and 15 Hz electroacupuncture (EA), respectively, to the right (ipsilateral) Zusanli (St-3...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/129875 |
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| author | Hsin-Cheng Hsu Nou-Ying Tang Yi-Wen Lin Tsai-Chung Li Hsu-Jan Liu Ching-Liang Hsieh |
| author_facet | Hsin-Cheng Hsu Nou-Ying Tang Yi-Wen Lin Tsai-Chung Li Hsu-Jan Liu Ching-Liang Hsieh |
| author_sort | Hsin-Cheng Hsu |
| collection | DOAJ |
| description | We adopt the chronic constriction injury (CCI) model to induce neuropathic pain to Spragrue-Dawley (SD) rats by ligating the right sciatic nerve of using four 4-0 chromic gut sutures and subsequently applying 2 and 15 Hz electroacupuncture (EA), respectively, to the right (ipsilateral) Zusanli (St-36) and Shangjuxu (St-37) acupoints. The results of this study are summarized as follows: (1) the differences in withdrawal latencies for the radiant heat test and total lift leg counts for the cold plate test (4°C) of the control (i.e., non-EA) and sham groups were greater than those of the 2 Hz EA (2EA) and 15 Hz EA (15EA) groups; (2) the von Frey test filament gram counts of the control and sham groups were less than those of the 2EA and 15EA groups on the 6th, 7th, 8th, 11th, 12th, and 13th day following ligation; and (3) the 2EA and 15EA groups exhibited reduced cerebral transient receptor potential vanilloid type 4 (TRPV4) expressions, although we did not observe a similar effect for cerebral TRPV1 or spinal TRPV4/TRPV1 expressions. These findings show that 2 and 15 Hz EA can reduce CCI-induced neuropathic pain, which indicates that various spinal segmental and gate effects have a crucial function in pain reduction. The relationship between EA and TRPV4/TRPV1 expression requires further study. |
| format | Article |
| id | doaj-art-8289c9d76e484ad689aa94fb76cdf9ce |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-8289c9d76e484ad689aa94fb76cdf9ce2025-08-20T02:08:31ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/129875129875Effect of Electroacupuncture on Rats with Chronic Constriction Injury-Induced Neuropathic PainHsin-Cheng Hsu0Nou-Ying Tang1Yi-Wen Lin2Tsai-Chung Li3Hsu-Jan Liu4Ching-Liang Hsieh5Graduate Institute of Chinese Medicine, College of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, TaiwanSchool of Chinese Medicine, College of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, TaiwanGraduate Institute of Acupuncture Science, College of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, TaiwanGraduate Institute of Biostatistics, College of Management, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, TaiwanGraduate Institute of Acupuncture Science, College of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, TaiwanDepartment of Chinese Medicine, China Medical University Hospital, 2 Yuh Der Road, Taichung 40402, TaiwanWe adopt the chronic constriction injury (CCI) model to induce neuropathic pain to Spragrue-Dawley (SD) rats by ligating the right sciatic nerve of using four 4-0 chromic gut sutures and subsequently applying 2 and 15 Hz electroacupuncture (EA), respectively, to the right (ipsilateral) Zusanli (St-36) and Shangjuxu (St-37) acupoints. The results of this study are summarized as follows: (1) the differences in withdrawal latencies for the radiant heat test and total lift leg counts for the cold plate test (4°C) of the control (i.e., non-EA) and sham groups were greater than those of the 2 Hz EA (2EA) and 15 Hz EA (15EA) groups; (2) the von Frey test filament gram counts of the control and sham groups were less than those of the 2EA and 15EA groups on the 6th, 7th, 8th, 11th, 12th, and 13th day following ligation; and (3) the 2EA and 15EA groups exhibited reduced cerebral transient receptor potential vanilloid type 4 (TRPV4) expressions, although we did not observe a similar effect for cerebral TRPV1 or spinal TRPV4/TRPV1 expressions. These findings show that 2 and 15 Hz EA can reduce CCI-induced neuropathic pain, which indicates that various spinal segmental and gate effects have a crucial function in pain reduction. The relationship between EA and TRPV4/TRPV1 expression requires further study.http://dx.doi.org/10.1155/2014/129875 |
| spellingShingle | Hsin-Cheng Hsu Nou-Ying Tang Yi-Wen Lin Tsai-Chung Li Hsu-Jan Liu Ching-Liang Hsieh Effect of Electroacupuncture on Rats with Chronic Constriction Injury-Induced Neuropathic Pain The Scientific World Journal |
| title | Effect of Electroacupuncture on Rats with Chronic Constriction Injury-Induced Neuropathic Pain |
| title_full | Effect of Electroacupuncture on Rats with Chronic Constriction Injury-Induced Neuropathic Pain |
| title_fullStr | Effect of Electroacupuncture on Rats with Chronic Constriction Injury-Induced Neuropathic Pain |
| title_full_unstemmed | Effect of Electroacupuncture on Rats with Chronic Constriction Injury-Induced Neuropathic Pain |
| title_short | Effect of Electroacupuncture on Rats with Chronic Constriction Injury-Induced Neuropathic Pain |
| title_sort | effect of electroacupuncture on rats with chronic constriction injury induced neuropathic pain |
| url | http://dx.doi.org/10.1155/2014/129875 |
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