Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available Iron
Chlamydia trachomatis is a leading cause of sexually transmitted infection worldwide and responsible for myriad of immunopathological changes associated with reproductive health. Delayed secretion of proinflammatory chemokine interleukin (IL)-8 is a hallmark of chlamydial infection and is dependent...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2009/417658 |
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| author | Harsh Vardhan Raini Dutta Vikas Vats Rishein Gupta Rajneesh Jha Hem Chandra Jha Pragya Srivastava Apurb Rashmi Bhengraj Aruna Singh Mittal |
| author_facet | Harsh Vardhan Raini Dutta Vikas Vats Rishein Gupta Rajneesh Jha Hem Chandra Jha Pragya Srivastava Apurb Rashmi Bhengraj Aruna Singh Mittal |
| author_sort | Harsh Vardhan |
| collection | DOAJ |
| description | Chlamydia trachomatis is a leading cause of sexually transmitted infection worldwide and responsible for myriad of immunopathological changes associated with reproductive health. Delayed secretion of proinflammatory chemokine interleukin (IL)-8 is a hallmark of chlamydial infection and is dependent on chlamydial growth. We examined the effect of iron chelators on IL-8 production in HeLa 229 (cervix epitheloid cell, CCL2) cells infected with C. trachomatis. IL-8 production was induced by Iron chelator DFO and Mimosine, however, synergy with chlamydial infection was obtained with DFO only. Temporal expression of proinflammatory secreted cytokines IL-1beta, TNF-alpha, and IL-8 did not show synchrony in Chlamydia trachomatis infected cells. Secretion of IL-8 from Hela cells infected with C. trachomatis was not dependent on IL-1 beta and TNF- alpha induction. These results indicate towards involvement of iron in chlamydia induced IL-8 production. |
| format | Article |
| id | doaj-art-827ec608abba404ea33403a74c66eb4e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-827ec608abba404ea33403a74c66eb4e2025-08-20T03:37:31ZengWileyMediators of Inflammation0962-93511466-18612009-01-01200910.1155/2009/417658417658Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available IronHarsh Vardhan0Raini Dutta1Vikas Vats2Rishein Gupta3Rajneesh Jha4Hem Chandra Jha5Pragya Srivastava6Apurb Rashmi Bhengraj7Aruna Singh Mittal8Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, IndiaInstitute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, IndiaInstitute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, IndiaInstitute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, IndiaInstitute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, IndiaInstitute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, IndiaInstitute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, IndiaInstitute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, IndiaInstitute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, IndiaChlamydia trachomatis is a leading cause of sexually transmitted infection worldwide and responsible for myriad of immunopathological changes associated with reproductive health. Delayed secretion of proinflammatory chemokine interleukin (IL)-8 is a hallmark of chlamydial infection and is dependent on chlamydial growth. We examined the effect of iron chelators on IL-8 production in HeLa 229 (cervix epitheloid cell, CCL2) cells infected with C. trachomatis. IL-8 production was induced by Iron chelator DFO and Mimosine, however, synergy with chlamydial infection was obtained with DFO only. Temporal expression of proinflammatory secreted cytokines IL-1beta, TNF-alpha, and IL-8 did not show synchrony in Chlamydia trachomatis infected cells. Secretion of IL-8 from Hela cells infected with C. trachomatis was not dependent on IL-1 beta and TNF- alpha induction. These results indicate towards involvement of iron in chlamydia induced IL-8 production.http://dx.doi.org/10.1155/2009/417658 |
| spellingShingle | Harsh Vardhan Raini Dutta Vikas Vats Rishein Gupta Rajneesh Jha Hem Chandra Jha Pragya Srivastava Apurb Rashmi Bhengraj Aruna Singh Mittal Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available Iron Mediators of Inflammation |
| title | Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available Iron |
| title_full | Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available Iron |
| title_fullStr | Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available Iron |
| title_full_unstemmed | Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available Iron |
| title_short | Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available Iron |
| title_sort | persistently elevated level of il 8 in chlamydia trachomatis infected hela 229 cells is dependent on intracellular available iron |
| url | http://dx.doi.org/10.1155/2009/417658 |
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