Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model
Summary: The lack of an etiologic Parkinson’s disease (PD) model in non-human primates has inhibited our understanding of the disease and the development of therapies. Here, we present a protocol for generating a PD model in Macaca fascicularis. We describe steps for localizing the substantia nigra...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000279 |
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| author | Shuyi Liu Wei Si Zhengbo Wang |
| author_facet | Shuyi Liu Wei Si Zhengbo Wang |
| author_sort | Shuyi Liu |
| collection | DOAJ |
| description | Summary: The lack of an etiologic Parkinson’s disease (PD) model in non-human primates has inhibited our understanding of the disease and the development of therapies. Here, we present a protocol for generating a PD model in Macaca fascicularis. We describe steps for localizing the substantia nigra and striatum using magnetic resonance imaging (MRI). We then detail a technique for the intracerebral administration of adeno-associated virus (AAV)-mediated overexpression of α-synuclein into the substantia nigra plus the injection of poly(ADP-ribose) (PAR) into the striatum.For complete details on the use and execution of this protocol, please refer to Liu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-824f7d9a195445d69637ce99972d256d |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-824f7d9a195445d69637ce99972d256d2025-02-07T04:48:20ZengElsevierSTAR Protocols2666-16672025-03-0161103621Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey modelShuyi Liu0Wei Si1Zhengbo Wang2State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, ChinaState Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China; Yunnan Key Laboratory of Primate Biomedical Research, Kunming, Yunnan 650500, China; Corresponding authorState Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China; Yunnan Key Laboratory of Primate Biomedical Research, Kunming, Yunnan 650500, China; Corresponding authorSummary: The lack of an etiologic Parkinson’s disease (PD) model in non-human primates has inhibited our understanding of the disease and the development of therapies. Here, we present a protocol for generating a PD model in Macaca fascicularis. We describe steps for localizing the substantia nigra and striatum using magnetic resonance imaging (MRI). We then detail a technique for the intracerebral administration of adeno-associated virus (AAV)-mediated overexpression of α-synuclein into the substantia nigra plus the injection of poly(ADP-ribose) (PAR) into the striatum.For complete details on the use and execution of this protocol, please refer to Liu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000279NeuroscienceCognitive NeuroscienceNMR |
| spellingShingle | Shuyi Liu Wei Si Zhengbo Wang Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model STAR Protocols Neuroscience Cognitive Neuroscience NMR |
| title | Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model |
| title_full | Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model |
| title_fullStr | Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model |
| title_full_unstemmed | Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model |
| title_short | Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model |
| title_sort | protocol for the intracerebral injection of aav α synuclein plus poly adp ribose to generate a parkinson s disease monkey model |
| topic | Neuroscience Cognitive Neuroscience NMR |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000279 |
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