Analyzing Antibiotic Resistance in Clinical Mycobacterium tuberculosis Isolates using Microplate Alamar Blue Assay
Introduction: Tuberculosis, caused by Mycobacterium tuberculosis, is one of the most common infectious diseases worldwide. Epidemiological studies of M. tuberculosis drug resistance are critical for improving patient treatment approaches and controlling the spread of tuberculosis. The present stu...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2023-12-01
|
| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Introduction: Tuberculosis, caused by Mycobacterium tuberculosis, is one
of the most common infectious diseases worldwide. Epidemiological studies
of M. tuberculosis drug resistance are critical for improving patient treatment
approaches and controlling the spread of tuberculosis. The present study
aimed to determine antibiotic resistance among M. tuberculosis clinical
isolates using the Microplate Alamar Blue Assay (MABA). Methods: In this
descriptive cross-sectional study, 25 M. tuberculosis isolates from clinical
samples were identified and confirmed using standard microbiological and
biochemical tests. Then, the MIC for the antibiotics Bedaquiline, isoniazid,
rifampin, ethambutol, ofloxacin, moxifloxacin, capreomycin, and
streptomycin was determined using the MABA method. The results were
analyzed using SPSS version 16 software. Results: Among the 25
investigated isolates, the frequencies for MDR, Pre-XDR, and XDR isolates
were 20%, 8%, and 32%, respectively. The highest rate of drug resistance
was to isoniazid (80%), rifampicin, and ethambutol (76%), and the highest
rate of sensitivity was to moxifloxacin (68%). The frequency of isoniazid
mono-resistance and rifampicin mono-resistance was 5 cases (50%) and 4
cases (40%), respectively. Conclusion: Our study revealed an alarming rate
of MDR and XDR M. tuberculosis strains, indicating that current first-line
treatments may be ineffective for a significant number of patients. The
bedaquiline resistance among the isolates with no history of previous
exposure to this drug suggests unexplored resistance mechanisms. Molecular
techniques to accurately identify these mechanisms may contribute to
developing more effective treatment strategies to combat drug-resistant
tuberculosis. |
|---|---|
| ISSN: | 2345-5349 2345-5330 |