The effect of cold plasma treatment on the phenolic and flavonoid content and antioxidant activity of whole buckwheat grain and flour

Abstract Atmospheric cold plasma (ACP) is a novel non-thermal technology which increases shelf-life of food. The aim of this research to evaluate the effects of ACP treatment on antioxidant activity (AOA), total phenol content (TPC), total flavonoid content (TFC) and rutin and quercetin levels of Co...

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Main Authors: Mahsa Amiri, Masoumeh Arab, Amir Mohammad Mortazavian
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-10281-x
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author Mahsa Amiri
Masoumeh Arab
Amir Mohammad Mortazavian
author_facet Mahsa Amiri
Masoumeh Arab
Amir Mohammad Mortazavian
author_sort Mahsa Amiri
collection DOAJ
description Abstract Atmospheric cold plasma (ACP) is a novel non-thermal technology which increases shelf-life of food. The aim of this research to evaluate the effects of ACP treatment on antioxidant activity (AOA), total phenol content (TPC), total flavonoid content (TFC) and rutin and quercetin levels of Common buckwheat (Fagopyrum esculentum Moench) flour (BF) and grain (BG). Whole buckwheat grains and flour were subjected to a dielectric barrier discharge plasma reactor for 5 and 10 min at 50 and 60 kV. Results demonstrated the significant impact of ACP treatment on phenolics, flavonoids, especially rutin, and antioxidant activity. BF sample treated at 50 kV-10 min (S2), and BG sample treated at 60 kV-5 min (S3) showed the most AOA (92.25 ± 0.03, 89.69 ± 0.04% by DPPH and, 48.09 ± 0.05, 42.88 ± 0.03 mmol Fe2+/mg DW by FRAP), TPC (83.99 ± 0.07, 80.47 ± 0.03 mg GAE/g DW), TFC (96.60 ± 0.03, 91.53 ± 0.07 mg RE/g DW), and rutin (3.6 ± 0.06, 2.7 ± 0.02 mg/kg) levels, respectively. Results indicated that optimum ACP treatment can increase TPC, TFC, and AOA of buckwheat.
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spelling doaj-art-823bd12016844da2a0a118b4f0ff29442025-08-20T03:42:49ZengNature PortfolioScientific Reports2045-23222025-07-011511810.1038/s41598-025-10281-xThe effect of cold plasma treatment on the phenolic and flavonoid content and antioxidant activity of whole buckwheat grain and flourMahsa Amiri0Masoumeh Arab1Amir Mohammad Mortazavian2Department of Food Science and Technology, School of Public Health, Shahid Sadoughi University of Medical SciencesDepartment of Food Science and Technology, School of Public Health, Shahid Sadoughi University of Medical SciencesDepartment of Food Science and Technology, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical SciencesAbstract Atmospheric cold plasma (ACP) is a novel non-thermal technology which increases shelf-life of food. The aim of this research to evaluate the effects of ACP treatment on antioxidant activity (AOA), total phenol content (TPC), total flavonoid content (TFC) and rutin and quercetin levels of Common buckwheat (Fagopyrum esculentum Moench) flour (BF) and grain (BG). Whole buckwheat grains and flour were subjected to a dielectric barrier discharge plasma reactor for 5 and 10 min at 50 and 60 kV. Results demonstrated the significant impact of ACP treatment on phenolics, flavonoids, especially rutin, and antioxidant activity. BF sample treated at 50 kV-10 min (S2), and BG sample treated at 60 kV-5 min (S3) showed the most AOA (92.25 ± 0.03, 89.69 ± 0.04% by DPPH and, 48.09 ± 0.05, 42.88 ± 0.03 mmol Fe2+/mg DW by FRAP), TPC (83.99 ± 0.07, 80.47 ± 0.03 mg GAE/g DW), TFC (96.60 ± 0.03, 91.53 ± 0.07 mg RE/g DW), and rutin (3.6 ± 0.06, 2.7 ± 0.02 mg/kg) levels, respectively. Results indicated that optimum ACP treatment can increase TPC, TFC, and AOA of buckwheat.https://doi.org/10.1038/s41598-025-10281-xCommon buckwheatCold plasmaAntioxidant activityRutin
spellingShingle Mahsa Amiri
Masoumeh Arab
Amir Mohammad Mortazavian
The effect of cold plasma treatment on the phenolic and flavonoid content and antioxidant activity of whole buckwheat grain and flour
Scientific Reports
Common buckwheat
Cold plasma
Antioxidant activity
Rutin
title The effect of cold plasma treatment on the phenolic and flavonoid content and antioxidant activity of whole buckwheat grain and flour
title_full The effect of cold plasma treatment on the phenolic and flavonoid content and antioxidant activity of whole buckwheat grain and flour
title_fullStr The effect of cold plasma treatment on the phenolic and flavonoid content and antioxidant activity of whole buckwheat grain and flour
title_full_unstemmed The effect of cold plasma treatment on the phenolic and flavonoid content and antioxidant activity of whole buckwheat grain and flour
title_short The effect of cold plasma treatment on the phenolic and flavonoid content and antioxidant activity of whole buckwheat grain and flour
title_sort effect of cold plasma treatment on the phenolic and flavonoid content and antioxidant activity of whole buckwheat grain and flour
topic Common buckwheat
Cold plasma
Antioxidant activity
Rutin
url https://doi.org/10.1038/s41598-025-10281-x
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