Antimicrobial Susceptibility of Commensal <i>Escherichia coli</i> from Pig Fecal Samples and Enhanced Sensitivity for Direct Detection of the <i>bla</i><sub>CTX-M</sub> Gene by Nested PCR

Antimicrobial Susceptibility of Commensal <i>Escherichia coli</i> from Pig Fecal Samples and Enhanced Sensitivity for Direct Detection of the <i>bla</i><sub>CTX-M</sub> Gene by Nested PCR

The commensal <i>Escherichia coli</i> in the gut of pigs is a major reservoir of antimicrobial resistance and can result in possible transmission to humans through the food chain. Direct detection of <i>E. coli</i> from fecal samples is challenging and can be used as a bioind...

Full description

Saved in:
Bibliographic Details
Main Authors: Nutchaba Suchanta, Naeem Ullah, Pitak Santanirand, Nutthee Am-In, Nuntaree Chaichanawongsaroj
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Animals
Subjects:
<i>E. coli</i>
ESBLs
antimicrobial resistance
<i>bla</i><sub>CTX-M</sub> gene
nPCR
pig feces
Online Access:https://www.mdpi.com/2076-2615/14/18/2630
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The commensal <i>Escherichia coli</i> in the gut of pigs is a major reservoir of antimicrobial resistance and can result in possible transmission to humans through the food chain. Direct detection of <i>E. coli</i> from fecal samples is challenging and can be used as a bioindicator of antimicrobial resistance. This study aimed to compare the antimicrobial susceptibility profiles in commensal <i>E. coli</i> from antibiotic- and nonantibiotic-using pig farms and developed the direct detection of ESBL genes in pig fecal samples using nested PCR (nPCR) and multiplex PCR (mPCR) techniques. All direct genotypic results were validated with the results of PCR sequencing of isolated <i>E. coli</i> colonies. The ESBL-producing <i>E. coli</i> were found in 98.6% (145 isolates) and 96.6% (144 isolates) of antibiotic-using and nonantibiotic-using farms, respectively, predominantly CTX-M-55. The nPCR decreased the limit of detection (LOD) from sPCR about 100 times, and the lower LODs of 10<sup>2</sup>, 10<sup>1</sup>, and 1 CFU/mL were reached after incubating samples in an enrichment medium for 2, 4, and 8 h, respectively. The mPCR, sPCR, and nPCR techniques showed sensitivities of 30.15%, 69.85%, and 91.91%, respectively, compared to PCR sequencing. The stability and recycling of ESBL genes were independent of antibiotic usage in commensal <i>E. coli</i> originating in pig farms.
ISSN:2076-2615

Similar Items