Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex
Trehalose and glycogen metabolism plays an important role in supporting life processes in many nematodes, including Anisakis simplex. Nematodes, cosmopolitan helminths parasitizing sea mammals and humans, cause a disease known as anisakiasis. The aim of this study was to investigate the expression o...
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| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/438145 |
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| author | E. Łopieńska-Biernat E. A. Zaobidna M. Dmitryjuk |
| author_facet | E. Łopieńska-Biernat E. A. Zaobidna M. Dmitryjuk |
| author_sort | E. Łopieńska-Biernat |
| collection | DOAJ |
| description | Trehalose and glycogen metabolism plays an important role in supporting life processes in many nematodes, including Anisakis simplex. Nematodes, cosmopolitan helminths parasitizing sea mammals and humans, cause a disease known as anisakiasis. The aim of this study was to investigate the expression of genes encoding the enzymes involved in the metabolism of trehalose and glycogen—trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP), glycogen synthase (GS), and glycogen phosphorylase (GP)—in stage L3 and stage L4 larvae of A. simplex. The expression of mRNA all four genes, tps, tpp, gs, and gp, was examined by real-time polymerase chain reaction. The A. simplex ribosomal gene (18S) was used as a reference gene. Enzymatic activity was determined. The expression of trehalose enzyme genes was higher in L3 than in L4 larvae, but an inverse relationship was noted for the expression of gs and gp genes. |
| format | Article |
| id | doaj-art-8233434ba618407e9f72bc751fe541cd |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-8233434ba618407e9f72bc751fe541cd2025-02-03T06:08:01ZengWileyJournal of Parasitology Research2090-00232090-00312015-01-01201510.1155/2015/438145438145Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplexE. Łopieńska-Biernat0E. A. Zaobidna1M. Dmitryjuk2Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Oczapowskiego 1A, 10-917 Olsztyn, PolandDepartment of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Oczapowskiego 1A, 10-917 Olsztyn, PolandDepartment of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Oczapowskiego 1A, 10-917 Olsztyn, PolandTrehalose and glycogen metabolism plays an important role in supporting life processes in many nematodes, including Anisakis simplex. Nematodes, cosmopolitan helminths parasitizing sea mammals and humans, cause a disease known as anisakiasis. The aim of this study was to investigate the expression of genes encoding the enzymes involved in the metabolism of trehalose and glycogen—trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP), glycogen synthase (GS), and glycogen phosphorylase (GP)—in stage L3 and stage L4 larvae of A. simplex. The expression of mRNA all four genes, tps, tpp, gs, and gp, was examined by real-time polymerase chain reaction. The A. simplex ribosomal gene (18S) was used as a reference gene. Enzymatic activity was determined. The expression of trehalose enzyme genes was higher in L3 than in L4 larvae, but an inverse relationship was noted for the expression of gs and gp genes.http://dx.doi.org/10.1155/2015/438145 |
| spellingShingle | E. Łopieńska-Biernat E. A. Zaobidna M. Dmitryjuk Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex Journal of Parasitology Research |
| title | Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex |
| title_full | Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex |
| title_fullStr | Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex |
| title_full_unstemmed | Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex |
| title_short | Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex |
| title_sort | expression of genes encoding the enzymes for glycogen and trehalose metabolism in l3 and l4 larvae of anisakis simplex |
| url | http://dx.doi.org/10.1155/2015/438145 |
| work_keys_str_mv | AT ełopienskabiernat expressionofgenesencodingtheenzymesforglycogenandtrehalosemetabolisminl3andl4larvaeofanisakissimplex AT eazaobidna expressionofgenesencodingtheenzymesforglycogenandtrehalosemetabolisminl3andl4larvaeofanisakissimplex AT mdmitryjuk expressionofgenesencodingtheenzymesforglycogenandtrehalosemetabolisminl3andl4larvaeofanisakissimplex |