From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function in <i>Monascus</i> Species
<i>Monascus</i>, a genus of fungi known for its fermentation capability and production of bioactive compounds, such as <i>Monascus</i> azaphilone pigments and Monacolin K, have received considerable attention because of their potential in biotechnological applications. Unders...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/10/12/892 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850051005924769792 |
|---|---|
| author | Yunxia Gong Shengfa Li Deqing Zhao Xi Yuan Yin Zhou Fusheng Chen Yanchun Shao |
| author_facet | Yunxia Gong Shengfa Li Deqing Zhao Xi Yuan Yin Zhou Fusheng Chen Yanchun Shao |
| author_sort | Yunxia Gong |
| collection | DOAJ |
| description | <i>Monascus</i>, a genus of fungi known for its fermentation capability and production of bioactive compounds, such as <i>Monascus</i> azaphilone pigments and Monacolin K, have received considerable attention because of their potential in biotechnological applications. Understanding the genetic basis of these metabolic pathways is crucial for optimizing the fermentation and enhancing the yield and quality of these products. However, <i>Monascus</i> spp. are not model fungi, and knowledge of their genetics is limited, which is a great challenge in understanding physiological and biochemical phenomena at the genetic level. Since the first application of particle bombardment to explore gene function, it has become feasible to link the phenotypic variation and genomic information on <i>Monascus</i> strains. In recent decades, accurate gene editing assisted by genomic information has provided a solution to analyze the functions of genes involved in the metabolism and development of <i>Monascus</i> spp. at the molecular level. This review summarizes most of the genetic manipulation tools used in <i>Monascus</i> spp. and emphasizes <i>Agrobacterium tumefaciens</i>-mediated transformation and nuclease-guided gene editing, providing comprehensive references for scholars to select suitable genetic manipulation tools to investigate the functions of genes of interest in <i>Monascus</i> spp. |
| format | Article |
| id | doaj-art-8230a1622f654c4484611fd5dfacd7fa |
| institution | DOAJ |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-8230a1622f654c4484611fd5dfacd7fa2025-08-20T02:53:17ZengMDPI AGJournal of Fungi2309-608X2024-12-01101289210.3390/jof10120892From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function in <i>Monascus</i> SpeciesYunxia Gong0Shengfa Li1Deqing Zhao2Xi Yuan3Yin Zhou4Fusheng Chen5Yanchun Shao6College of Food Science and Technology, Wuhan Business University, Wuhan 430056, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Wuhan Business University, Wuhan 430056, ChinaCollege of Food Science and Technology, Wuhan Business University, Wuhan 430056, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China<i>Monascus</i>, a genus of fungi known for its fermentation capability and production of bioactive compounds, such as <i>Monascus</i> azaphilone pigments and Monacolin K, have received considerable attention because of their potential in biotechnological applications. Understanding the genetic basis of these metabolic pathways is crucial for optimizing the fermentation and enhancing the yield and quality of these products. However, <i>Monascus</i> spp. are not model fungi, and knowledge of their genetics is limited, which is a great challenge in understanding physiological and biochemical phenomena at the genetic level. Since the first application of particle bombardment to explore gene function, it has become feasible to link the phenotypic variation and genomic information on <i>Monascus</i> strains. In recent decades, accurate gene editing assisted by genomic information has provided a solution to analyze the functions of genes involved in the metabolism and development of <i>Monascus</i> spp. at the molecular level. This review summarizes most of the genetic manipulation tools used in <i>Monascus</i> spp. and emphasizes <i>Agrobacterium tumefaciens</i>-mediated transformation and nuclease-guided gene editing, providing comprehensive references for scholars to select suitable genetic manipulation tools to investigate the functions of genes of interest in <i>Monascus</i> spp.https://www.mdpi.com/2309-608X/10/12/892<i>Monascus</i> spp.genetic transformationsite-specific gene editingmultiple-gene perturbations |
| spellingShingle | Yunxia Gong Shengfa Li Deqing Zhao Xi Yuan Yin Zhou Fusheng Chen Yanchun Shao From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function in <i>Monascus</i> Species Journal of Fungi <i>Monascus</i> spp. genetic transformation site-specific gene editing multiple-gene perturbations |
| title | From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function in <i>Monascus</i> Species |
| title_full | From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function in <i>Monascus</i> Species |
| title_fullStr | From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function in <i>Monascus</i> Species |
| title_full_unstemmed | From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function in <i>Monascus</i> Species |
| title_short | From Random Perturbation to Precise Targeting: A Comprehensive Review of Methods for Studying Gene Function in <i>Monascus</i> Species |
| title_sort | from random perturbation to precise targeting a comprehensive review of methods for studying gene function in i monascus i species |
| topic | <i>Monascus</i> spp. genetic transformation site-specific gene editing multiple-gene perturbations |
| url | https://www.mdpi.com/2309-608X/10/12/892 |
| work_keys_str_mv | AT yunxiagong fromrandomperturbationtoprecisetargetingacomprehensivereviewofmethodsforstudyinggenefunctioninimonascusispecies AT shengfali fromrandomperturbationtoprecisetargetingacomprehensivereviewofmethodsforstudyinggenefunctioninimonascusispecies AT deqingzhao fromrandomperturbationtoprecisetargetingacomprehensivereviewofmethodsforstudyinggenefunctioninimonascusispecies AT xiyuan fromrandomperturbationtoprecisetargetingacomprehensivereviewofmethodsforstudyinggenefunctioninimonascusispecies AT yinzhou fromrandomperturbationtoprecisetargetingacomprehensivereviewofmethodsforstudyinggenefunctioninimonascusispecies AT fushengchen fromrandomperturbationtoprecisetargetingacomprehensivereviewofmethodsforstudyinggenefunctioninimonascusispecies AT yanchunshao fromrandomperturbationtoprecisetargetingacomprehensivereviewofmethodsforstudyinggenefunctioninimonascusispecies |