High-dose radiation induces dendritic cells maturation by promoting immunogenic cell death in nasopharyngeal carcinoma
Aim and backgroundDue to the radiosensitivity and deep anatomical location of nasopharyngeal carcinoma (NPC), radiotherapy serves as the cornerstone of standardized treatment for this malignancy. Beyond its cytotoxic effects, radiotherapy can serve as an immunological adjuvant by inducing immunogeni...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1554018/full |
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| author | Xianlin Zeng Xianlin Zeng Xianlin Zeng Xianhuai Jin Xianhuai Jin Ji Leng Ji Leng Ji Leng Shuai Zhang Yun Wang Yun Wang Yun Wang Jin Chen Jin Chen Jin Chen Shichao Zhang Shichao Zhang Shichao Zhang Lijing Teng Lijing Teng Lijing Teng Zuquan Hu Zuquan Hu Zuquan Hu Shi Zhou Zhu Zeng Zhu Zeng Zhu Zeng Zhu Zeng Zhu Zeng Jinhua Long Jinhua Long Jinhua Long |
| author_facet | Xianlin Zeng Xianlin Zeng Xianlin Zeng Xianhuai Jin Xianhuai Jin Ji Leng Ji Leng Ji Leng Shuai Zhang Yun Wang Yun Wang Yun Wang Jin Chen Jin Chen Jin Chen Shichao Zhang Shichao Zhang Shichao Zhang Lijing Teng Lijing Teng Lijing Teng Zuquan Hu Zuquan Hu Zuquan Hu Shi Zhou Zhu Zeng Zhu Zeng Zhu Zeng Zhu Zeng Zhu Zeng Jinhua Long Jinhua Long Jinhua Long |
| author_sort | Xianlin Zeng |
| collection | DOAJ |
| description | Aim and backgroundDue to the radiosensitivity and deep anatomical location of nasopharyngeal carcinoma (NPC), radiotherapy serves as the cornerstone of standardized treatment for this malignancy. Beyond its cytotoxic effects, radiotherapy can serve as an immunological adjuvant by inducing immunogenic cell death (ICD). Dendritic cells (DCs), as potent antigen-presenting cells, play a critical role in tumor immunotherapy, but their exact role in the ICD process of NPC remains unclear. The effects of high-dose radiation (≥2 Gy) on DCs and the type of immune response it elicits in NPC have not been fully elucidated.MethodsAn in vitro study was conducted to assess whether ICD of NPC 5-8F cells induced by high-dose radiation could regulate the immune response of DCs. Specifically, the maturation and antigen-presenting capacity of DCs were evaluated following co-culture with NPC cells exposed to high-dose radiation.ResultsHigh-dose radiation was found to induce ICD in NPC 5-8F cells, as evidenced by increased pro-inflammatory factor levels and reduced anti-inflammatory factor levels in the cell culture supernatant. Co-culture with NPC cells exposed to high-dose radiation for 15 minutes significantly enhanced the expression of surface molecules on DCs, promoting their immune sensitization.ConclusionHigh-dose radiation-induced apoptosis of NPC 5-8F cells is a form of ICD, which plays an
important role in regulating DC immune function. These findings provide insight into the immunomodulatory effects of radiotherapy in NPC and its potential to enhance tumor immunotherapy through DC activation. |
| format | Article |
| id | doaj-art-822e6b67da6441f68f98d17a41f62713 |
| institution | DOAJ |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-822e6b67da6441f68f98d17a41f627132025-08-20T02:43:16ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-02-011610.3389/fimmu.2025.15540181554018High-dose radiation induces dendritic cells maturation by promoting immunogenic cell death in nasopharyngeal carcinomaXianlin Zeng0Xianlin Zeng1Xianlin Zeng2Xianhuai Jin3Xianhuai Jin4Ji Leng5Ji Leng6Ji Leng7Shuai Zhang8Yun Wang9Yun Wang10Yun Wang11Jin Chen12Jin Chen13Jin Chen14Shichao Zhang15Shichao Zhang16Shichao Zhang17Lijing Teng18Lijing Teng19Lijing Teng20Zuquan Hu21Zuquan Hu22Zuquan Hu23Shi Zhou24Zhu Zeng25Zhu Zeng26Zhu Zeng27Zhu Zeng28Zhu Zeng29Jinhua Long30Jinhua Long31Jinhua Long32School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, ChinaEngineering Center of Cellular Immunotherapy of Guizhou Province, Guiyang, ChinaKey Laboratory of Infectious Immunity and Antibody Engineering of Guizhou Province, Guiyang, ChinaDepartment of Oncology, Guiyang Public Health Clinical Center, Guiyang, Guizhou, ChinaDepartment of Oncology, School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, ChinaSchool of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, ChinaEngineering Center of Cellular Immunotherapy of Guizhou Province, Guiyang, ChinaKey Laboratory of Infectious Immunity and Antibody Engineering of Guizhou Province, Guiyang, ChinaDepartment of Interventional Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang, ChinaSchool of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, ChinaEngineering Center of Cellular Immunotherapy of Guizhou Province, Guiyang, ChinaKey Laboratory of Infectious Immunity and Antibody Engineering of Guizhou Province, Guiyang, ChinaSchool of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, ChinaEngineering Center of Cellular Immunotherapy of Guizhou Province, Guiyang, ChinaKey Laboratory of Infectious Immunity and Antibody Engineering of Guizhou Province, Guiyang, ChinaSchool of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, ChinaEngineering Center of Cellular Immunotherapy of Guizhou Province, Guiyang, ChinaKey Laboratory of Infectious Immunity and Antibody Engineering of Guizhou Province, Guiyang, ChinaSchool of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, ChinaEngineering Center of Cellular Immunotherapy of Guizhou Province, Guiyang, ChinaKey Laboratory of Infectious Immunity and Antibody Engineering of Guizhou Province, Guiyang, ChinaSchool of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, ChinaEngineering Center of Cellular Immunotherapy of Guizhou Province, Guiyang, ChinaKey Laboratory of Infectious Immunity and Antibody Engineering of Guizhou Province, Guiyang, ChinaDepartment of Interventional Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang, ChinaSchool of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang, ChinaEngineering Center of Cellular Immunotherapy of Guizhou Province, Guiyang, ChinaKey Laboratory of Infectious Immunity and Antibody Engineering of Guizhou Province, Guiyang, ChinaKey Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang, ChinaState Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, ChinaDepartment of Oncology, School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, ChinaDepartment of Oncology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China0Department of Oncology, Affiliated Cancer Hospital of Guizhou Medical University, Guiyang, Guizhou, ChinaAim and backgroundDue to the radiosensitivity and deep anatomical location of nasopharyngeal carcinoma (NPC), radiotherapy serves as the cornerstone of standardized treatment for this malignancy. Beyond its cytotoxic effects, radiotherapy can serve as an immunological adjuvant by inducing immunogenic cell death (ICD). Dendritic cells (DCs), as potent antigen-presenting cells, play a critical role in tumor immunotherapy, but their exact role in the ICD process of NPC remains unclear. The effects of high-dose radiation (≥2 Gy) on DCs and the type of immune response it elicits in NPC have not been fully elucidated.MethodsAn in vitro study was conducted to assess whether ICD of NPC 5-8F cells induced by high-dose radiation could regulate the immune response of DCs. Specifically, the maturation and antigen-presenting capacity of DCs were evaluated following co-culture with NPC cells exposed to high-dose radiation.ResultsHigh-dose radiation was found to induce ICD in NPC 5-8F cells, as evidenced by increased pro-inflammatory factor levels and reduced anti-inflammatory factor levels in the cell culture supernatant. Co-culture with NPC cells exposed to high-dose radiation for 15 minutes significantly enhanced the expression of surface molecules on DCs, promoting their immune sensitization.ConclusionHigh-dose radiation-induced apoptosis of NPC 5-8F cells is a form of ICD, which plays an important role in regulating DC immune function. These findings provide insight into the immunomodulatory effects of radiotherapy in NPC and its potential to enhance tumor immunotherapy through DC activation.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1554018/fullradiotherapynasopharyngeal carcinomaimmune responseimmunogenic cell deathdendritic cells |
| spellingShingle | Xianlin Zeng Xianlin Zeng Xianlin Zeng Xianhuai Jin Xianhuai Jin Ji Leng Ji Leng Ji Leng Shuai Zhang Yun Wang Yun Wang Yun Wang Jin Chen Jin Chen Jin Chen Shichao Zhang Shichao Zhang Shichao Zhang Lijing Teng Lijing Teng Lijing Teng Zuquan Hu Zuquan Hu Zuquan Hu Shi Zhou Zhu Zeng Zhu Zeng Zhu Zeng Zhu Zeng Zhu Zeng Jinhua Long Jinhua Long Jinhua Long High-dose radiation induces dendritic cells maturation by promoting immunogenic cell death in nasopharyngeal carcinoma Frontiers in Immunology radiotherapy nasopharyngeal carcinoma immune response immunogenic cell death dendritic cells |
| title | High-dose radiation induces dendritic cells maturation by promoting immunogenic cell death in nasopharyngeal carcinoma |
| title_full | High-dose radiation induces dendritic cells maturation by promoting immunogenic cell death in nasopharyngeal carcinoma |
| title_fullStr | High-dose radiation induces dendritic cells maturation by promoting immunogenic cell death in nasopharyngeal carcinoma |
| title_full_unstemmed | High-dose radiation induces dendritic cells maturation by promoting immunogenic cell death in nasopharyngeal carcinoma |
| title_short | High-dose radiation induces dendritic cells maturation by promoting immunogenic cell death in nasopharyngeal carcinoma |
| title_sort | high dose radiation induces dendritic cells maturation by promoting immunogenic cell death in nasopharyngeal carcinoma |
| topic | radiotherapy nasopharyngeal carcinoma immune response immunogenic cell death dendritic cells |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1554018/full |
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