Genotypic and demographic characterization of invasive isolates of Salmonella Typhimurium in HIV co-infected patients in South Africa
BACKGROUND: Non-typhoidal Salmonella are frequently food-borne zoonotic pathogens that may cause invasive disease in HIV-positive individuals. METHODOLOGY: Invasive isolates (n = 652) of Salmonella Typhimurium from human patients in Gauteng Province of South Africa were investigated for the years 20...
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The Journal of Infection in Developing Countries
2009-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/549 |
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| author | Karen H. Keddy Sarika Dwarika Penny Crowther Olga Perovic Jeanette Wadula Anwar Hoosen Arvinda Sooka Heather H. Crewe-Brown Anthony M. Smith |
| author_facet | Karen H. Keddy Sarika Dwarika Penny Crowther Olga Perovic Jeanette Wadula Anwar Hoosen Arvinda Sooka Heather H. Crewe-Brown Anthony M. Smith |
| author_sort | Karen H. Keddy |
| collection | DOAJ |
| description | BACKGROUND: Non-typhoidal Salmonella are frequently food-borne zoonotic pathogens that may cause invasive disease in HIV-positive individuals.
METHODOLOGY: Invasive isolates (n = 652) of Salmonella Typhimurium from human patients in Gauteng Province of South Africa were investigated for the years 2006 and 2007. Bacteria were identified using standard microbiological techniques and serotyping was performed using commercially available antisera. Susceptibility testing to antimicrobial agents was determined by the E-test. Genotypic relatedness of isolates was investigated by pulsed-field gel electrophoresis analysis of digested genomic DNA.
RESULTS: Forty-five clusters of isolates were identified, of which four (clusters 3, 5, 6 and 11) were major clusters. Most isolates originated from hospital H2 and most were isolated from patients in the age range of 15 to 64 years. Ninety-three percent (213/230) of patients with a known HIV status were HIV-positive. Most isolates showed resistance to multiple antibiotics. The most commonly expressed antibiotic resistance profiles were ACSSuNa (14%; 75/555) and ACSSuTNa (13%; 72/555). Some evidence was found for nosocomial acquisition of isolates. Of the isolates from the major clusters 3, 5, 6, and 11, 33% (8/24), 6% (7/117), 4% (1/26) and 6% (3/52) were of nosocomial origin, respectively.
CONCLUSIONS: In South Africa, Salmonella Typhimurium remains a major opportunistic infection of predominantly HIV-positive patients. Clonally diverse strains that are resistant to multiple antibiotics may circulate in patients aged between 15 and 64 years over prolonged periods within the hospital environment, adding to the health care burden. |
| format | Article |
| id | doaj-art-822d74af1585494bb4e4cf3e9c9904f6 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2009-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-822d74af1585494bb4e4cf3e9c9904f62025-08-20T03:48:58ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802009-09-0130810.3855/jidc.549Genotypic and demographic characterization of invasive isolates of Salmonella Typhimurium in HIV co-infected patients in South AfricaKaren H. Keddy0Sarika Dwarika1Penny Crowther2Olga Perovic3Jeanette Wadula4Anwar Hoosen5Arvinda Sooka6Heather H. Crewe-Brown7Anthony M. Smith8Enteric Diseases Reference Unit, National Institute for Communicable Diseases, JohannesburgEnteric Diseases Reference Unit, National Institute for Communicable Diseases, JohannesburgNational Microbiology Surveillance Unit, National Institute for Communicable Diseases, JohannesburgDepartment of Clinical Microbiology and Infectious Diseases, National Health Laboratory Service, University of Witwatersrand, JohannesburgDepartment of Clinical Microbiology and Infectious Diseases, National Health Laboratory Service, University of Witwatersrand, JohannesburgDepartment of Medical Microbiology, University of Pretoria and National Health Laboratory Service Tshwane Academic Division, PretoriaEnteric Diseases Reference Unit, National Institute for Communicable Diseases, JohannesburgDepartment of Clinical Microbiology and Infectious Diseases, National Health Laboratory Service, University of Witwatersrand, JohannesburgDepartment of Clinical Microbiology and Infectious Diseases, National Health Laboratory Service, University of Witwatersrand, JohannesburgBACKGROUND: Non-typhoidal Salmonella are frequently food-borne zoonotic pathogens that may cause invasive disease in HIV-positive individuals. METHODOLOGY: Invasive isolates (n = 652) of Salmonella Typhimurium from human patients in Gauteng Province of South Africa were investigated for the years 2006 and 2007. Bacteria were identified using standard microbiological techniques and serotyping was performed using commercially available antisera. Susceptibility testing to antimicrobial agents was determined by the E-test. Genotypic relatedness of isolates was investigated by pulsed-field gel electrophoresis analysis of digested genomic DNA. RESULTS: Forty-five clusters of isolates were identified, of which four (clusters 3, 5, 6 and 11) were major clusters. Most isolates originated from hospital H2 and most were isolated from patients in the age range of 15 to 64 years. Ninety-three percent (213/230) of patients with a known HIV status were HIV-positive. Most isolates showed resistance to multiple antibiotics. The most commonly expressed antibiotic resistance profiles were ACSSuNa (14%; 75/555) and ACSSuTNa (13%; 72/555). Some evidence was found for nosocomial acquisition of isolates. Of the isolates from the major clusters 3, 5, 6, and 11, 33% (8/24), 6% (7/117), 4% (1/26) and 6% (3/52) were of nosocomial origin, respectively. CONCLUSIONS: In South Africa, Salmonella Typhimurium remains a major opportunistic infection of predominantly HIV-positive patients. Clonally diverse strains that are resistant to multiple antibiotics may circulate in patients aged between 15 and 64 years over prolonged periods within the hospital environment, adding to the health care burden.https://jidc.org/index.php/journal/article/view/549Salmonella TyphimuriumHIVinvasivenosocomialSouth Africa |
| spellingShingle | Karen H. Keddy Sarika Dwarika Penny Crowther Olga Perovic Jeanette Wadula Anwar Hoosen Arvinda Sooka Heather H. Crewe-Brown Anthony M. Smith Genotypic and demographic characterization of invasive isolates of Salmonella Typhimurium in HIV co-infected patients in South Africa Journal of Infection in Developing Countries Salmonella Typhimurium HIV invasive nosocomial South Africa |
| title | Genotypic and demographic characterization of invasive isolates of Salmonella Typhimurium in HIV co-infected patients in South Africa |
| title_full | Genotypic and demographic characterization of invasive isolates of Salmonella Typhimurium in HIV co-infected patients in South Africa |
| title_fullStr | Genotypic and demographic characterization of invasive isolates of Salmonella Typhimurium in HIV co-infected patients in South Africa |
| title_full_unstemmed | Genotypic and demographic characterization of invasive isolates of Salmonella Typhimurium in HIV co-infected patients in South Africa |
| title_short | Genotypic and demographic characterization of invasive isolates of Salmonella Typhimurium in HIV co-infected patients in South Africa |
| title_sort | genotypic and demographic characterization of invasive isolates of salmonella typhimurium in hiv co infected patients in south africa |
| topic | Salmonella Typhimurium HIV invasive nosocomial South Africa |
| url | https://jidc.org/index.php/journal/article/view/549 |
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