Protozoocidal activity of Stemona collinsiae against Giardia duodenalis
Giardia duodenalis is a major pathogen of the gastrointestinal tract, and infections impact both human health and the economy. The ongoing issues with drug resistance and the side effects of current anti-Giardia treatments highlight the urgent need for new therapeutic options. This study focused on...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024175615 |
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| author | Khuanchai Koompapong Supaluk Popruk Onrapak Reamtong Tipparat Thiangtrongjit Sumate Ampawong Aurapa Sakulpanich Ruenruetai Udonsom Kanthinich Thima Preeyaporn M. Sreepian Kittiyod Poovorawan Hirotake Mori Christen Rune Stensvold Aongart Mahittikorn |
| author_facet | Khuanchai Koompapong Supaluk Popruk Onrapak Reamtong Tipparat Thiangtrongjit Sumate Ampawong Aurapa Sakulpanich Ruenruetai Udonsom Kanthinich Thima Preeyaporn M. Sreepian Kittiyod Poovorawan Hirotake Mori Christen Rune Stensvold Aongart Mahittikorn |
| author_sort | Khuanchai Koompapong |
| collection | DOAJ |
| description | Giardia duodenalis is a major pathogen of the gastrointestinal tract, and infections impact both human health and the economy. The ongoing issues with drug resistance and the side effects of current anti-Giardia treatments highlight the urgent need for new therapeutic options. This study focused on investigating the in vitro efficacy of crude extracts of Stemona collinsiae from Thailand against G. duodenalis. S. collinsiae was initially extracted with hexane, followed sequentially by dichloromethane, ethanol, and water. The extracts were tested for growth inhibition against G. duodenalis. Dichloromethane and hexane extracts were highly active against the parasite after 48 h of exposure, with half-maximal inhibitory concentration (IC50) values of 60.77 μg/mL and 66.66 μg/mL, respectively. Both extracts reduced G. duodenalis trophozoite motility and adherence, altered trophozoite architecture, and induced apoptosis. Our findings demonstrate the anti-Giardia activity of S. collinsiae root extracts, especially those of dichloromethane and hexane. Our results will support the potential development of new and effective medications for the treatment of giardiasis. |
| format | Article |
| id | doaj-art-82251e67c3334323a6404421ec157c8c |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-82251e67c3334323a6404421ec157c8c2025-01-17T04:51:33ZengElsevierHeliyon2405-84402025-01-01111e41530Protozoocidal activity of Stemona collinsiae against Giardia duodenalisKhuanchai Koompapong0Supaluk Popruk1Onrapak Reamtong2Tipparat Thiangtrongjit3Sumate Ampawong4Aurapa Sakulpanich5Ruenruetai Udonsom6Kanthinich Thima7Preeyaporn M. Sreepian8Kittiyod Poovorawan9Hirotake Mori10Christen Rune Stensvold11Aongart Mahittikorn12Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, ThailandDepartment of Protozoology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, ThailandDepartment of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, ThailandDepartment of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, ThailandDepartment of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, ThailandDivision of Pharmaceutical Sciences, Faculty of Pharmacy, Thammasat University, Rangsit Campus, Pathum Thani, 12120, ThailandDepartment of Protozoology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, ThailandDepartment of Protozoology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, ThailandFaculty of Medical Technology, Rangsit University, Pathum Thani, 12000, ThailandDepartment of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, ThailandDepartment of General Medicine, Juntendo University Faculty of Medicine, Tokyo, JapanDepartment of Bacteria, Parasites and Fungi, Laboratory of Parasitology, Statens Serum Institut, Copenhagen, DenmarkDepartment of Protozoology, Faculty of Tropical Medicine, Mahidol University, Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand; Corresponding author.Giardia duodenalis is a major pathogen of the gastrointestinal tract, and infections impact both human health and the economy. The ongoing issues with drug resistance and the side effects of current anti-Giardia treatments highlight the urgent need for new therapeutic options. This study focused on investigating the in vitro efficacy of crude extracts of Stemona collinsiae from Thailand against G. duodenalis. S. collinsiae was initially extracted with hexane, followed sequentially by dichloromethane, ethanol, and water. The extracts were tested for growth inhibition against G. duodenalis. Dichloromethane and hexane extracts were highly active against the parasite after 48 h of exposure, with half-maximal inhibitory concentration (IC50) values of 60.77 μg/mL and 66.66 μg/mL, respectively. Both extracts reduced G. duodenalis trophozoite motility and adherence, altered trophozoite architecture, and induced apoptosis. Our findings demonstrate the anti-Giardia activity of S. collinsiae root extracts, especially those of dichloromethane and hexane. Our results will support the potential development of new and effective medications for the treatment of giardiasis.http://www.sciencedirect.com/science/article/pii/S2405844024175615Giardia duodenalisHuman healthStemona collinsiaeIn vitro activityHerbal medicineDrug development |
| spellingShingle | Khuanchai Koompapong Supaluk Popruk Onrapak Reamtong Tipparat Thiangtrongjit Sumate Ampawong Aurapa Sakulpanich Ruenruetai Udonsom Kanthinich Thima Preeyaporn M. Sreepian Kittiyod Poovorawan Hirotake Mori Christen Rune Stensvold Aongart Mahittikorn Protozoocidal activity of Stemona collinsiae against Giardia duodenalis Heliyon Giardia duodenalis Human health Stemona collinsiae In vitro activity Herbal medicine Drug development |
| title | Protozoocidal activity of Stemona collinsiae against Giardia duodenalis |
| title_full | Protozoocidal activity of Stemona collinsiae against Giardia duodenalis |
| title_fullStr | Protozoocidal activity of Stemona collinsiae against Giardia duodenalis |
| title_full_unstemmed | Protozoocidal activity of Stemona collinsiae against Giardia duodenalis |
| title_short | Protozoocidal activity of Stemona collinsiae against Giardia duodenalis |
| title_sort | protozoocidal activity of stemona collinsiae against giardia duodenalis |
| topic | Giardia duodenalis Human health Stemona collinsiae In vitro activity Herbal medicine Drug development |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024175615 |
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