Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000
Here, we present the development of an automated AmpliSeq™ (ThermoFischer, MA, USA) workflow for library building using the Biomek® 3000 Laboratory Automation Workstation (Beckman Coulter Inc., CA, USA), in which the total volume of PCR reagents and reagents for library preparation are reduced by on...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-06-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2019-0156 |
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| author | Maryam Sharafi Farzad Brian Møllegaard Pedersen Helle Smidt Mogensen Claus Børsting |
| author_facet | Maryam Sharafi Farzad Brian Møllegaard Pedersen Helle Smidt Mogensen Claus Børsting |
| author_sort | Maryam Sharafi Farzad |
| collection | DOAJ |
| description | Here, we present the development of an automated AmpliSeq™ (ThermoFischer, MA, USA) workflow for library building using the Biomek® 3000 Laboratory Automation Workstation (Beckman Coulter Inc., CA, USA), in which the total volume of PCR reagents and reagents for library preparation are reduced by one-half. The automated AmpliSeq workflow was tested using 43 stain samples (blood, bone, muscle tissue, semen, swab, nail scrape and cigarette butts) collected from crime scenes. The sequencing data were evaluated for locus balance, heterozygous allele balance and noise. The performance of libraries built with the automated AmpliSeq workflow using one-half of the recommended reagent volumes were similar to the performance of libraries built with the recommended (full) volumes of the reagents. |
| format | Article |
| id | doaj-art-822083c3c7cb4b0bbe70c2e059d574b2 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-822083c3c7cb4b0bbe70c2e059d574b22025-08-20T02:26:05ZengTaylor & Francis GroupBioTechniques0736-62051940-98182020-06-0168634234410.2144/btn-2019-0156Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000Maryam Sharafi Farzad0Brian Møllegaard Pedersen1Helle Smidt Mogensen2Claus Børsting31Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health & Medical Sciences, University of Copenhagen, Copenhagen, Denmark1Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health & Medical Sciences, University of Copenhagen, Copenhagen, Denmark1Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health & Medical Sciences, University of Copenhagen, Copenhagen, Denmark1Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health & Medical Sciences, University of Copenhagen, Copenhagen, DenmarkHere, we present the development of an automated AmpliSeq™ (ThermoFischer, MA, USA) workflow for library building using the Biomek® 3000 Laboratory Automation Workstation (Beckman Coulter Inc., CA, USA), in which the total volume of PCR reagents and reagents for library preparation are reduced by one-half. The automated AmpliSeq workflow was tested using 43 stain samples (blood, bone, muscle tissue, semen, swab, nail scrape and cigarette butts) collected from crime scenes. The sequencing data were evaluated for locus balance, heterozygous allele balance and noise. The performance of libraries built with the automated AmpliSeq workflow using one-half of the recommended reagent volumes were similar to the performance of libraries built with the recommended (full) volumes of the reagents.https://www.future-science.com/doi/10.2144/btn-2019-0156AmpliSeq™ workflowBiomek® 3000library buildingmassively parallel sequencing |
| spellingShingle | Maryam Sharafi Farzad Brian Møllegaard Pedersen Helle Smidt Mogensen Claus Børsting Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000 BioTechniques AmpliSeq™ workflow Biomek® 3000 library building massively parallel sequencing |
| title | Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000 |
| title_full | Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000 |
| title_fullStr | Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000 |
| title_full_unstemmed | Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000 |
| title_short | Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000 |
| title_sort | development of an automated ampliseq™ library building workflow for biological stain samples on the biomek r 3000 |
| topic | AmpliSeq™ workflow Biomek® 3000 library building massively parallel sequencing |
| url | https://www.future-science.com/doi/10.2144/btn-2019-0156 |
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